OBJECTIVE:To analysis the micro-policy demand of medical institutions about carrying on qualified phar?maceutical service.METHODS:To compare the difference of pharmaceutical service and the policy between domestic and ov_ ersea medical institutions.RESULTS&CONCLUSION:To develop the pharmaceutical service of our country,the governm_ ent and authority should establish the encouraging and regulative policies about education,medicine,licensed pharmacist,personnel matters management,and so on.

Positive and meaningful exploration was conducted by related teachers to improve the teaching quality of oral clinical pharmacology.Case based teaching method was applied in the teaching of adverse reactions and bilingual teaching was employed in the teaching of local anesthesia.Correlation and comparison of the key knowledge points were emphasized in the teaching process. Students′ interests were aroused and satisfactory results were achieved through implementing these measures.

Bone and Bones ; drug effects ; metabolism ; Humans ; Lead ; adverse effects ; Osteogenesis ; drug effects

Information Services ; Occupational Health Services ; organization & administration

Drug Resistance, Viral ; genetics ; Hepatitis B virus ; drug effects ; genetics ; Mutation

Congenital Abnormalities ; Encephalocele ; Humans ; Nose Diseases ; congenital

Multidrug resistant genes are highly expressed in hepatocellular carcinoma that seriousty affects the effect of chemotherapy. Screening of resistant genes from HCC cells and studying its mechanism of drug resistance will be helpful to improve the effecacy of chemotherapy for hepatocellular carcinoma. Here we described an alternative method called cyclical packaging rescue (CPR). First we constructed a retrovirus cDNA library of hepatoma cells and used it to infect fibroblasts. Then we added drugs to screen survival cells. The survival cells, stably integrated helper-free retroviral libraries, were recovered rapidly after transfection with plasmids expressing retroviral gag-pol and env genes. Through this method, retroviral RNAs were directly repackaged into new infectious virions. Recovered retroviral supernatant was then used to reinfect fresh target cells. When performed in concert with selection using functional assays, cDNAs regulating functional responses could be identified by enrichment through multiple rounds of retroviral library recovery and retransmission. Using CPR, we obtained several cDNAs. After a preliminary detection, we found Ribosomal protein S11 (RPS11), Ribosomal protein L6 (RPL6), Ribosomal protein L11 (RPL11), Ribosomal protein L24 (RPL24) possibly had drug resistant function.
Carcinoma, Hepatocellular ; genetics ; pathology ; Cell Line, Tumor ; DNA, Complementary ; Drug Resistance, Neoplasm ; genetics ; Gene Library ; Genetic Vectors ; Humans ; Liver Neoplasms ; genetics ; pathology ; Plasmids ; Retroviridae ; Ribosomal Proteins ; genetics ; metabolism ; Transfection

OBJECTIVE:To establish a method for analyzing the volatile oil of high-yield and high-oil Curcuma kwangsiensis, and to provide reference for its breeding. METHODS:GC-MS was performed on the column of HP-5MS quartz elastic capillary column,carrier gas was high purity helium(99.999%),flow rate was 1.0 ml/min,inlet temperature was 250 ℃,the initial tem-perature of column was 50 ℃(temperature programmed),split injection with split ratio of 10:1. Mass spectrometry conditions:ionization mode was the electron impact ion source,ionization energy was 70 eV,the ion source temperature was 230 ℃,the quadrupole temperature was 150 ℃, transmission line temperature was 280 ℃,the electron multiplier voltage was 1588 V,and mass scanning range was m/z 45 to 500. High-yield and high-oil germplasm of were screened from 100 different germplasms,the volatile oil composition of single plant and relative percentage contents of each composition were compared,SPSS 22.0 software was used for cluster analysis. RESULTS:Totally 10 high-yield and high-oil germplasm were screened,54 kinds of compounds were identified,the common compositions of 10 different germplasms were camphor,1-caryophyllene,γ-elemene,curcumene, gemma ketone and new curdione,most germplasms contained borneol,isoborneol,δ-elemene,germacrene and calamine. The C78,C104,purpose 2,volatile oil content and relative percentage contents of active compositions in volatile oil were high. The 10 high-yield and high-oil germplasms can be divided into 3 groups. CONCLUSIONS:The study basically clears the main chemicalcomposition of volatile oil of high-yield and high-oil C. kwangsiensis,C78,C104 and purpose 2 are the more excellent strains in high-yield and high-oil C. Kwangsiensis germplasm.

0.05), the ratio of Cm/Cps rose gradually with lowering of rectal temperature and it reached the top value at 28℃; during this period AST, ALT, Cr and BUN decreased with dropping of rectal temperature (P

Objective To investigate the possibility of laparoscopic cholecystectomy(LC) on porcelain gallbladder.Methods Twenty-four cases of porcelain gallbladder,who were operated in China Medical University,including 13 LC cases,from 2006 to 2008 were retrospectively reviewed.Results Of 24 porcelain gallbladder cases(0.48%) in 4964 cholecystectomy patients,calcification of gallbladder in 87.50%(21/24) patients was diagnosed by ultrasonography.Of 13 patients who were cured by LC,one suffered from postoperative leak bile,no metastasis were found by following up 12 or 14 months in two gallbladder carcinoma cases.Conclusion There's specificity of ultrasonographic image in porcelain gallbladder,in which LC is safe to be performed and routine frozen pathology during operation is necessary.