1.The Macro-Policy Demand of Medical Institutions about Perfecting Pharmaceutical Service
China Pharmacy 2001;0(10):-
OBJECTIVE:To analysis the micro-policy demand of medical institutions about carrying on qualified phar?maceutical service.METHODS:To compare the difference of pharmaceutical service and the policy between domestic and ov_ ersea medical institutions.RESULTS&CONCLUSION:To develop the pharmaceutical service of our country,the governm_ ent and authority should establish the encouraging and regulative policies about education,medicine,licensed pharmacist,personnel matters management,and so on.
3.Analysis on the practical teaching reform in oral clinical pharmacology
Chinese Journal of Medical Education Research 2012;11(4):375-377
Positive and meaningful exploration was conducted by related teachers to improve the teaching quality of oral clinical pharmacology.Case based teaching method was applied in the teaching of adverse reactions and bilingual teaching was employed in the teaching of local anesthesia.Correlation and comparison of the key knowledge points were emphasized in the teaching process. Students′ interests were aroused and satisfactory results were achieved through implementing these measures.
7.Screening of drug resistent gene by cyclical packaging rescue of hepatocellular carcinoma retroviral cDNA libraries.
Wenyan DAI ; Ruiyu ZHU ; Jian JIN
Chinese Journal of Biotechnology 2016;32(2):204-211
Multidrug resistant genes are highly expressed in hepatocellular carcinoma that seriousty affects the effect of chemotherapy. Screening of resistant genes from HCC cells and studying its mechanism of drug resistance will be helpful to improve the effecacy of chemotherapy for hepatocellular carcinoma. Here we described an alternative method called cyclical packaging rescue (CPR). First we constructed a retrovirus cDNA library of hepatoma cells and used it to infect fibroblasts. Then we added drugs to screen survival cells. The survival cells, stably integrated helper-free retroviral libraries, were recovered rapidly after transfection with plasmids expressing retroviral gag-pol and env genes. Through this method, retroviral RNAs were directly repackaged into new infectious virions. Recovered retroviral supernatant was then used to reinfect fresh target cells. When performed in concert with selection using functional assays, cDNAs regulating functional responses could be identified by enrichment through multiple rounds of retroviral library recovery and retransmission. Using CPR, we obtained several cDNAs. After a preliminary detection, we found Ribosomal protein S11 (RPS11), Ribosomal protein L6 (RPL6), Ribosomal protein L11 (RPL11), Ribosomal protein L24 (RPL24) possibly had drug resistant function.
Carcinoma, Hepatocellular
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genetics
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pathology
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Cell Line, Tumor
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DNA, Complementary
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Drug Resistance, Neoplasm
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genetics
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Gene Library
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Genetic Vectors
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Humans
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Liver Neoplasms
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genetics
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pathology
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Plasmids
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Retroviridae
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Ribosomal Proteins
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genetics
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metabolism
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Transfection
8.Comparison of levobupivacaine and bupivacaine in spinal anesthesia during transurethral resection of prostate for elderly patients
Jian LI ; Guoguang JIN ; Weidong YE
Chinese Journal of General Practitioners 2011;10(7):501-503
Eighty elderly patients undergoing elective resection for the prostate were randomly divided into two groups, the levobupivacaine (observe group) and bupivacaine (control group) were used for spinal anesthesia, respectively. The level of sensory block and maintain time were T7±4 and (224 ±28) min in observed group, those in control group were T6±3 and (227 ±30) min, respectively ( both P> 0. 05). The mean arterial blood pressure of control group was (71 ±8) -(72 ±8) mm Hg (1 mm Hg = 0. 133 kPa) from 5 to 30 min after injection; meanwhile that of observed group was(75 ± 9) mm Hg (P < 0. 05). The incidences of hypotension and nausea were 10% (4/40) and 0% (0/40) respectively in observe group, and those were 30% (12/40) and 15% (6/40) respectively in control group (both P<0.05). The results indicate that levobupivacaine and bupivacaine have similar anesthetic effects, but levobupivacaine has fewer side effects and is more suitable for elderly patients.
10.Preparation and Quality Control of Podophyllotoxin Film Former
Jian CAO ; Huiping ZHONG ; Jin LI
China Pharmacy 2001;0(10):-
OBJECTIVE:To investigate the preparing process and method of quality control of the podophyllotoxin film former METHODS:To sift the formula of podophyllotoxin film former with orthogonal function and to determine the content by second derivative UV-spectrophotometry RESULTS:The peak area of podophyllotoxin against the concentration was linear with a correlation coefficient of 0 9 999 over the range of 80~180?g/ml and detecting at wavelength 301 5nm~315nm CONC_LUSION:The preparing process of podophyllotoxin film former is simple and the method of quality control is feasible