1.Clinical application of TSRH hook screw fixation after direct repair of lumbar spondylolysis in young patients
Jian FAN ; Jian ZHAO ; Fan LIU
Orthopedic Journal of China 2006;0(15):-
[Objective]To assess the clinical outcome of the clinical application of TSRH hook screw fixation after direct repair of lumbar spondylolysis in young patients.[Method]From 2002.7～2003.10,12 young male patients suffered from lumbarspondylolysis were selected to be treated with TSRH hook screw fixation after direct repair of the defect.MacNab criteria was used to assess their pre-and postoperative status.Postoperatively,radiographic finding and clinical results were observed.[Result]All patients were followed-up after surgery,ranging from 6 months to 19 months.Radiographs showed all patients having bilateral union;a11 patients with low back pain or radicular pain experienced significantly relieved.Neither complications nor instrumentation failure were observed.[Conclusion]This technique proves to be well-designed and easy to handle,and is considered useful and recommended for direct repair of the defects found in lumbar spondylolysis in young patients.
2.Effect of photodynamic therapy on the cell proliferation and collagen secretion of keloid fibroblasts.
Chinese Journal of Plastic Surgery 2015;31(1):49-53
OBJECTIVETo investigate the effect of 8-aminolevulinic acid (ALA) photodynamic therapy (PDT) on the cell proliferation, apoptosis and collagen secretion in keloid fibroblasts and to provide the theoretical base for ALA-PDT treatment of keloids.
METHODSFibroblasts from keloid patients were cultured to the third generation in vitro and incubated in 0, 1, 3, 6, 9 mmol/L of δ-aminolevulinic acid for 3 h in the darkness. Then they were exposed to 635 nm wavelength red light ( 30 J/cm2 ) and continued incubation 24 h after irradiation. CCK-8 assay was used to detect proliferation inhibition rate of fibroblasts. The content of hydroxyproline was measured by colorimetric method. The expression of p-Akt and programmed cell death 4 ( PDCD4) were detected by Western blot.
RESULTSThe inhibition rate of keloid fibroblasts were respectively 0, (8.30 ± 1.01)%, (29.48 ± 3.27)%, (52.01 ± 5.34)%, (79.99 ± 5.85)% with the presence of difference concentrations (0, 1, 3, 6, 9 mmol/L) of ALA. The content of hydroxyproline were respectively (9.540 0 ± 0.352 42), (6.242 5 ± 0.224 85 ), (5.107 5 ± 0.534 88), (3.490 0 ± 0.623 48), (2.945 0 ± 0.514 10) μg/mg. The relative expression of p-Akt were respectively 1, 0.75 ± 0.12, 0.52 ± 0.14, 0.41 ± 0.18, 0.32 ± 0.09. The relative expression of PDCD4 were respectively 1, 1.18 ± 0.19, 1.51 ± 0.22, 0.15 ± 0.30, 2.44 ± 0.22. The difference was statistically significant when compared the group of 1, 3, 6, 9 mmol/L with 0 mmol/L (P < 0.05).
CONCLUSIONSIn concentration within the range of 1-9 mmol/L, ALA could inhibit the proliferation of fibroblasts significantly, promote fibroblasts apoptosis and reduce the content of hydroxyproline in a dose-dependent manner, indicating that 8-aminolevulinic acid photodynamic therapy may be a potential treatment for keloid.
Aminolevulinic Acid ; pharmacology ; Apoptosis ; drug effects ; Cell Culture Techniques ; Cell Proliferation ; drug effects ; Collagen ; secretion ; Fibroblasts ; cytology ; drug effects ; secretion ; Humans ; Keloid ; drug therapy ; pathology ; Light ; Photochemotherapy ; methods ; Photosensitizing Agents ; pharmacology
6.The correlation between micrometastasis and SBEN expression in the peripheral blood of patients with breast cancer
Chinese Journal of General Surgery 2000;0(11):-
Objective To study the expression of small breast epithelial mucin(SBEM) in the peripheral blood of the patients with breast cancer,and explore the correlation between micrometastasis and SBEM.Methods Peripheral blood samples were obtained from 92 patients with breast carcinoma,20 patients with benign breast diseases,10 patients with malignant tumor not originating from breast,and 10 normal healthy volunteers.SBEM-mRNA expression was detected by nested reverse transcription-polymerase chain reaction(Nested-RT PCR).Results Among 92 breast carcinoma patients,43(46.7%) were Nested-RT-PCR positive for SBEM-mRNA,but it was negative in all the other groups.The expression of SBEM-mRNA in peripheral blood did not correlate with ER or PR status,menstruation state or primary tumour size.but correlated with patient′s age,the state of Her-2,CEA,CA15-3 and axillary lymph node metastasis of breast cancer.Conclusions The expression of SBEM-mRNA in peripheral blood of breast cancer can be of potential use to monitor the micrometastasis of breast cancer.
8.Diagnosis and treatment of traumatic abdominal wall hernia
Chinese Journal of General Surgery 2008;23(9):695-698
Objective To analyze the causes of misdiagnesis and mistreatment of traumatic abdominal hernia. Methods In this study,11 cases of traumatic abdominal hernia admitted during 1971 to 2004 were retrospectively analyzed.Hernia located subcutaneously in 8 cases including incarcerated hernia in 2 csses.Hernia located intermuscularly in 2 cases with one case being incarcerated.and the other strangulated.One located retroperitoneally.Among the 11 cases,3 were simple hernia,8 were complicated with injuries of other organs. Results Eight cases were misdiagnosed,5 were operated on with wrong incision,and in one case the herniated intestinal loop,mistaken as hematoma,was incised.All these 5 cases underwent a second surgery due to failure in repairing the hernia. Condusion For traumatic abdominal wall hernia,correct diagnosis,the correct timing of operation and an appropriate incision chosen insure good therapeutic results.
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