BACKGROUND:Kidney-nourishing and blood activating Chinese herbs in the No. Ⅰ recipe for ostooarthritis have the effects of treating osteoarthritis and preventing the side effect of glucocorticoid.OBJECTIVE: To build up rabbit chondrocyte culture system in vitro to investigate the effect of preventive effect of No. Ⅰ recipe for osteoarthritis on chondrocyte injury by hydrocortisone.DESIGN:A complete randomized design and controlled trial.SETTING: Department of Rheumatology and Immunology, Second Hospital of Fujian ProvinceMATERIALS:The experiment was conducted from November 2003 to March 2004 at the Molecular Laboratory of Department of Orthopedics &Grammatology, Fujian College of Traditional Chinese Medicine. No. Ⅰrecipe for osteoarthritis consisted of Herba Epimedii, Radix Morindae Officinalis, Rhizoma Ligustic Chuanxiong, Radix Notoginseng and Daphne geraldii Nitsche. Articular cartilage was digested with collagenase Ⅱ ,chondrocyte culture system in vitro was built up, then in the culture system the different concentrations of the mixture of No. Ⅰ recipe for osteoarthritis and hydrocortisone were added, according to the concentrations they were at random divided into two groups. One group was added 1.25 g/L hydrocortisone and/or 150 g/L No. Ⅰ recipe for osteoarthritis, the other group was added 2.50 g/L hydrocortisone and/or 300 g/L No. Ⅰ recipe for osteoarthritis. Each group was again subdivided as control, hydrocortisone, hydrocortisone + No. Ⅰ recipe for osteoarthritis and No. Ⅰ recipe for osteoarthritis groups, with 5 wells in each subgroup.teoarthritis group: In each well 0.1 mL mixture of 1.25 g/L hydrocortihydrocortisone group: In each well 0.1 mL mixture of phosphate buffer teoarthritis group: In each well No. 1 recipe for osteoarthritis 0.1mL was In each well 0.1 mL mixture of 2.50 g/L hydrocortisone and 300 g/L No.each well 0.1mL mixture of phosphate buffer and 2.50 g/L hydrocortisone after medicines were added, the morphological change of chondrocyte in all (A) value in each well was assayed with tetrazoline colorimetry as reflection of proliferation of the chondrocyte. MAIN OUTCOME MEASURES:Influence of No. Ⅰ recipe for osteoarthritis combined with hydrocortisone on chondrocyte proliferation in rabbits.croscope that the chondrocytes in the control group were mostly in multiangles or shuttle-like, and adhesive growth; those cultured for 24 hours with 1.25 and 2.50 g/L hydrocortisone were in pyknosis or flotation state.;those in the various concentrations of hydrocortisone +No. Ⅰ recipe for osteoarthritis, and No. Ⅰ recipe for osteoarthritis groups were mostly in multiangles or shuttle-like, and adhesive growth, without difference with those in 1.25 g/L hydrocortisone group than in the control group(0.007 6±0.0018,0.015 2±0.002 6,t=5.374 0,P＜0.01);it was obviously higher in the 1.25 g/L hydrocortisone +150 g/L .No. Ⅰ recipe for osteoarthritis and 150 g/L No. Ⅰ recipe for osteoarthritis groups than in the 1.25 g/L hydrocortisone group (0.065 6±0.016 9, 0.086 6±0.019 0, t=7.630 9, 9.255 9,P＜0.01); it was obviously lower in the 2.50g/L hydrocortisone group than in the control group 2(0.005 3±0.001 4,0.015 3±0.002 3 ,t=8.304 5,P＜0.01) ;it was obviously higher in the 2.50 g/L hydrocortisone +300 g/L No. Ⅰ recipe for osteoarthritis and 300 g/L No. Ⅰ recipe for osteoarthritis groups than in the 2.50 g/L hydrocortisone group (0.085 8±0.015 0,0.092 6 ±0.016 8, t=11.948 3,11.579 4, P ＜ 0.01).CONCLUSION :Hydrocortisone in higher concentration could cause serious injury or death of chondrocytes, however the chondrocyte grew well cultured in the mixture of hydrocortisone and traditional Chinese drug, No.Ⅰ recipe for osteoarthritis had the effect of preventing injury of chondrocyte from hydrocortisone.

Multiple myeloma (MM) is a genetically complex disease that is becoming more common in today' s aging population.In the 18th European Hematology Association (EHA) annual meeting,there were a number of key topics focusing on the development of MM from intraclonal heterogeneity,novel therapeutic agents,and relapse and refractory MM therapy,etc.

Multiple myeloma is the second most common hematological malignancy.In the 19th European Hematology Association (EHA) conference,there are a number of hot and key topics focused on developing of MM diagnosis criteria,response evaluation,treatment strategy for elderly and relapse/refractory myeloma patients,and maintenance therapy etc.

Multiple myeloma (MM) is the second most common hematological malignancy.In the 56th American Society of Hematology (ASH) annual meeting,there are mainly five hot and key topics focusing on development of MM treatment,including when is the opportunity of transplant-eligible patients in the era of novel therapy? Should continuous therapy be the standard therapeutic approach in MM? What is a practical approach to relapsed MM? How should the recurrence/refractory MM patients be managed and treated? And what are the approved agents and novel agents?

Objective To study the effect of simvastatin combined with trimetazidine on cardiac function and high-sensitivity C-reactive protein(hs-CRP) in patients with chronic heart failure.Methods 160 patients with chronic heart failure were divided into control group and the observation group according to the random number table.The control group was treated with diuretics,inotropic,vasodilators and other conventional treatment,on the basis of this,the observation group received simvastatin combined with trimetazidine treatment.The cardiac function and hs-CRP of the patients were observed,and the treatment effects were evaluated and analyzed.Results The total effective rate of the observation group was 82.50％,which was significandy higher than 52.50％ of the control group (x2 =16.4103,P ＜ 0.001).Compared with before treatment,IL-6,TNF-alpha and hs-CRP levels of two groups after treatment were significantly decreased (t =12.234,13.342,5.463,8.533,7.896,3.536,all P ＜ 0.05),and those in the observation group were significantly lower than the control group (t =4.653,3.976,3.674,all P ＜ 0.05).Conclusion Simvastatin combined with trimetazidine has good effect on the cardiac function of patients with chronic heart failure,and can effectively reduce the level of hs-CRP,it is worthy of clinical application.

GoPubMed is an intelligent search engine based on PubMed aiming at realizing visualization and post - processing the re-salts. The paper analyzes its performance from three aspects, including working principle, key technique and extended functions. As the result of the research, it reveals that GoPubMed classifies the retrieval results from PubMed by semantic classification tools based on se-mantic web, namely GO and MESH, therefore, it helps users find high related literature very quickly. The combination between semantic web and biomedical information retrieval is realized perfectly, and visualization statistical analysis is gradually unveiled from multiple an-gles.

The platelet membrance glycoprotein plays a key role in the adhesion,aggregation and release of platelet.It is the specific molecular marker for platelet activation.Many studies have confirmed that platelet membrane glycoprotein CD62P may be more sensitive and specific to reflect the occurrence of coronary artery development,CD62P level is positively correlated with the severity.A variety of traditional Chinese medicine can lower the CD62P level,inhibit platelet activation and the treatment play an important role in the treatment.

0.05). The serum levels of E_2 in patient group were significantly lower than those of the regular menses group (P0.05). The serum levels of E_2 were significantly higher in patient group after treatment (P0.05). The menstrual blood levels of NO, E_2 were significantly higher, and ET were significantly higher, respectively, in patient group after treatment (P

Based on the translational research measurement and backtracking model put forward in this paper,it takes the new drug approved to enter into the market by American FDA from 2006-2015 as the fundamental data to measure the knowledge relations among new drug products-core patent-scientific paper-fund assistance,and puts forward the policies and suggestions for promoting innovation of pharmaceutical products in China.

The incidence of pancreatic neuroendocrine neoplasms is progressively growing.The clinical manifestation and treatment of this disease are complicated.Multi disciplinary combination therapy is needed to improve prognosis and life quality of patients.