Quality education is the profound innovation of China education.Humanities quality education is the breakthrough and cut-in point that boost roundly the quality education in higher learning.It is an effective way to put up humanities quality education systematically and selectively in medical universities by means of lectures on humanities knowledge,for lectures on humanities knowledge can not only enhance the medical students' humanities quality thus forming their humanities quality,but also effectively help students to display their autonomy and the teachers to play the leading role.It is the prefect way for student to experience humanities spirit.

Objective　To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods　The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results　①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion　There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.

For stage Ⅱ colon cancer patients after surgery,the benefit of adjuvant chemotherapy remains controversial.Several studies indicate that the patients with high-risk stage Ⅱ colon cancer can benefit from adjuvant chemotherapy after surgery.According to the clinical and pathological features,the stage Ⅱcolon cancer patients including T4 lesion,perforation,peritumoral lymphovascular invasion can benefit from the adjuvant chemotherapy of oxaliplatin.In the horizontal of molecular and genetic levels,the stage Ⅱ colon cancer patients can express high microsatellite instability (MSI-H) and cannot benefit from the chemotherapy of Fluorouracil.The guiding functions of 18q loss of heterozygosity (18q LOH),Cx43 and gene expression profiling in adjuvant chemotherapy are still unclear,and need further study.

OBJECTIVE: To establish a RP-HPLC method by pre - column derivatization to determine the contents of mi-cronomicin sulfate.METHODS:Kromasil C18 column( 150mm X 4.6mm,5u),mobile phasewater-ice acetic acidmethanol(30 5 : 65) in 0.02mol/L sodium -heptanesulfonate. The detection wavelength was set at 330nm.RESULTS:The linear range was 0.0 976mg/ml-0.9 760mg/ml,(r = 0.9 999),the RSD of the replicate test was 1.0%(n = 6) .CONCLUSION:The method is specific,accurate and sensitive.

Our observation was done with conjunctival microcirculation, pulmona-ry rheography and renal rheography in 20 cases with acute glomerulonephri-tis. High resistance of pulmonary rheography was observed and ischemicchanges were found in renal rheography when spasm of the conjunctivalarterioles appeared in 20 cases. 58. 7% of 29 patients were abnormal duringradiorenographic examination. Most cases showed damage to both kidneys,indicating the kidney disfunctioning caus ed by renal circulatory disorderswhen patients were suffering from nephritis. Changes seen in conjunctival microcirculation, rheography and radio-renography were consistently abnormal at the acute stage. At the convalesc-ent stage, however, improvement to different degrees was gained. Therefore,these laboratory studies can be used as index for the selection of vasodila-tor drugs and for the justification of the prognosis of the disease.

OBJECTIVETo observe the expression of peripheral blood CD4+ T lymphocyte apoptosis gene in rheumatoid arthritis (RA) patients with cold dampness type (CDT), and to explore its correlation with clinical indicators of RA.

METHODSSixteen RA patients with CDT (as the RA group) and 16 healthy subjects (as the normal control group) were recruited. CD4 T cell apoptosis rate was detected in the RA group and the normal control group using FCM. mRNA expressions of fas, fasL, caspase-3, caspase-8, bcl-2, and bax were detected using RT-PCR. Correlations between the expression of apoptosis gene and clinical activity indicators of RA (ESR, CRP, RF, CCP, integrals for Chinese medial symptoms, morning stiffness time, joint tenderness number, joint swelling number, DAS28-3) were analyzed.

RESULTSThe apoptosis rate of CD4+ T was significantly lower in the RA group than in the control group [(2. 6 +/- 0.9) % vs. (7.7 +/- 1.3) %, P < 0.01]. mRNA expression levels of fas, fasL, caspase-8, caspase-3, and bax mRNA of CD4+ T significantly decreased, but bcl-2 mRNA expression increased in the RA group (P < 0.01). The apoptosis rate of CD4+ T was negatively correlated with ESR (P < 0.05). The mRNA expression of caspase-8 was negatively correlated with joint swelling number (P < 0.05). The mRNA expression of bcl-2 was negatively correlated with integrals for Chinese medial-symptoms and joint function classification (P < 0.01, P < 0.05).

CONCLUSIONApoptosis obstacle exists in peripheral blood CD4 +T lymphocyte of RA patients, and is closely related to disease activity.

Apoptosis ; Arthritis, Rheumatoid ; diagnosis ; metabolism ; pathology ; CD4-Positive T-Lymphocytes ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Fas Ligand Protein ; Humans ; RNA, Messenger

Aged ; Aged, 80 and over ; Blood Platelets ; cytology ; Coronary Disease ; blood ; Female ; Humans ; Hypertension ; blood ; Male ; Platelet Count

BACKGROUND: The exact pathogenesis of osteoarthritis is unclear. Studies have shown that macrophages in synovial tissue are involved in sliding-inflammation response and the production of cartilage matrix degradation enzyme. OBJECTIVE: Based on the important role of synovial macrophages in osteoarthritis, to review and summarize the major research achievements and important research methods in this field, laying a foundation for future research on synovial macrophages. METHODS: The first author used computer to search relevant literatures included in PubMed and VIP from 1999 to 2019. The search terms were “osteoarthritis, synovitis, synovial macrophages” in English and Chineserespectively. Manual screening was conducted according to the inclusion criteria, and irrelevant, repetitive and obsolete articles were excluded. Finally, 46 eligible articles were included.RESULTS AND CONCLUSION: There is an inflammatory response in the synovial tissue, and the severity of synovitis is closely related to osteoarthritis symptoms and progression. A large number of macrophages with different activation states are infiltrated in the synovial tissues of osteoarthritis patients at different stages. M1 macrophages are the main subgroup of macrophages involved in the production, cartilage degradation and osteophyte formation of osteoarthritis inflammatory factors, while M2 macrophages have a protective effect on osteoarthritis by secreting anti-inflammatory factors. Inhibiting the differentiation of macrophages into M1 type and promoting their M2 type differentiation can alleviate the pathological changes of osteoarthritis and delay the progression of osteoarthritis. It may be an effective method to treat osteoarthritis by regulating the polarization state of synovial macrophages.

Objective Toinvestigate the relationship between red blood cell volume distribution width (RDW) and acute coronary syndrome (ACS),and the correlation between RDW and degree of coronary artery stenosis. Methods A total of 221patients accepting coronary angiography were involved as ACS group and another 50 patients also accepting coronary angiography as normal control group. ACS group wasfurther divided into unstable angina (UA) group of 77 cases and acute myocardial infarction (AMI) group of 144 cases ,then their level of RDW was detected. According to Gensini score ,we divided the 221 positive cases of coronary angiography into ≤ 30 point group ,31-60 point group and > 60 point group and their level of RDW was detected. Results The level of RDW in ACS group(13.02 ± 0.68)was significantly higher than that innormal control group(12.62 ± 0.38),and the difference was statisticallysignificant(P < 0.05). And the difference between UA group(12.84 ± 0.68)and AMI group(13.12 ± 0.66)also showed statistical significance(P < 0.05). With the increase of Gensini score ,the level of RDW was also increased ,and the difference between each two groups was statistically significant (P < 0.05) except that between control group and ≤ 30 point group. Conclusions The level of RDW in ACS group and AMI group is significantly higher ,suggesting that RDW may be involved in the pathogenesis of acute coronary syndrome ,and the RDW level could be a potential indicator of ACS. RDW level is closely related to the severity of coronary stenosis and could be one of its predictors.