Objective: To study the effect of ischemic preconditioning on heat-shock protein 70 (HSP70) expression and the learning,memory functions after forebrain ischemia-reperfusion injury in gerbils. Methods: Gerbils (n=100) were evenly randomized into four groups: Sham group, ischemia-reperfusion (I/R) group, ischemia preconditioning (IP) group, and Cycloheximide + IP group (Cycloheximide was administered 30 min before IP). Transient forebrain ischemia-reperfusion model was established by bilateral common carotid artery occlusion according to the method described previously, and ischemia preconditioning model was established as described by Kitagawa. Changes of neuron morphous in hippocampus CA1 region were observed by H-E staining 1, 2, and 3 days after reperfusion. The expression of HSP70 was examined by immunohistochemistry and Western blotting assay. Neuron apoptosis was detected by TUNEL method. The learning/memory functions of gerbils were examined using 4-PTT dry path maze 3, 4, 5, 6, and 7 days after reperfusion. Results: Compared with Sham group, I/R group had significantly decreased survival neurons(P<0. 05), increased neuron apoptosis(P<0. 05), increased expression of HSP70 (P<0. 05), and decreased learning/memory functions(P<0. 05). Compared with I/R group, IP group had significantly increased survival neurons(P<0. 05), decreased neuron apoptosis (P<0. 05), and increased expression of HSP70 (P<0. 05), and improved learning/memory functions (P < 0. 05). However, cycloheximide almost totally abolished the effect of ischemia preconditioning, with the neuron morphology, density, apoptosis, HSP70 expression, and learning/memory functions similar to those of I/R group. Conclusion: Ischemic preconditioning can protect against cerebral ischemia injury and improve the learning/ memory functions after forebrain ischemia-reperfusion damage, which is possibly through promoting HSP70 expression and starting endogenous neuroprotective mechanism, subsequently reinforcing the protective effect against ischemia.

Aged, 80 and over ; Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Diagnosis, Differential ; Fasciitis ; pathology ; Fibroblasts ; pathology ; Fibrosarcoma ; metabolism ; pathology ; surgery ; Forearm ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Male ; Myxosarcoma ; metabolism ; pathology ; surgery ; Soft Tissue Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism ; alpha 1-Antichymotrypsin ; metabolism

Adult ; Carcinoma, Endometrioid ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Hysterectomy ; Ovarian Neoplasms ; pathology ; surgery ; Sex Cord-Gonadal Stromal Tumors ; pathology ; surgery

Objective To study the effect of baicalein on the skin cell light aging and ERK signaling pathways. Methods Using UVB phototherapy device, of which light intensity was 0.5 mJ/cm2, different exposure time was tried before establishing the best exposure time light aging model. The experiment can be divided into control group, model group and baicalein group (1 × 10-7, 1 × 10-6, 1 × 10-5 mol/L) since the results of preliminary experiments shown that these three concentration of baicalein is the most effective concentration. Using flow cytometry instrument to test cells reactive oxygen species (ROS) content changes of each group. quantitative Real-time RCR (qRT-PCR) and Western blotting were respectively used to detect mRNA and protein expression in each group. Results The best exposure time for 5 min, and cell shrinkage, damage, apoptosis were observed in model group. Compared with model group, cell damage, shrinking phenomenon obviously reduced in 1 × 10-7, 1 × 10-6 mol/L baicalein group, and cell obviously tended to normal form in 1 × 10-5 mol/L baicalein group. Compared with the control group, the ROS content of cells in model group was increased significantly (P < 0.01). Compared with model group, the ROS content was decreased significantly in 1 × 10-7, 1 × 10-6, 1 × 10-5 mol/L baicalein group (P < 0.01); Compared with the control group, the expression of IL-1α and IL-8 mRNA was increased significantly (P < 0.01), and the expression of IL-1-α, IL-8, and p-ERK protein was also increased significantly (P < 0.01) and ERK protein expression quantity remains the same in the model group. In 1 × 10-7, 1 × 10-6, 1 × 10-5 mol/L baicalein group, compared with model group, IL-1α and IL-8 mRNA expression were decreased significantly (P < 0.01), and IL-1-α, IL-8, and p-ERK protein expression were also decreased significantly (P < 0.05, 0.01) and ERK protein expression quantity remains the same. Conclusion Protection of baicalein on UVB induced cell light aging, mainly through reducing ROS content, blocking ERK signaling pathway and inhibiting inflammatory cytokines.

Objective Ouabain is a cardiotonic steroid that can induce the apoptosis of many tumorous cells .This study was to investigate the anti-tumor mechanisms of ouabain by observing its effects on the apoptosis of T lymphoblastic leukemia Jurkat cells and the expressions of hTERT and c-myc mRNA and protein . Methods Jurkat cells were treated with ouabain at the concentrations of 50 and 100 nmol/L for 24 and 48 hours, and those treated with 1 ×PBS served as the control .Then the apoptosis rate of the cells was detected by flow cytometry after Annexin V/PI staining, the expressions of hTERT and c-myc mRNA determined by RT-PCR, and those of hTERT and c-myc protein by Western blot . Results The apoptosis rates of the Jurkat cells in the 50 and 100 nmol/L oua-bain groups were (5.67 ±3.71)%and (9.63 ±4.83)%respectively at 24 hours, and (19.67 ±4.55)%and (37.60 ±11.89)%at 48 hours, significantly higher than (4.23 ±1.01)%in the PBS control group at 48 hours (P<0.05).Compared with the control, the expressions of hTERT and c-myc mRNA were decreased by 200%and those of hTERT and c-myc protein by 224%and 400%, re-spectively, at 48 hours (P<0.05).There was a positive correlation between the reduction of the mRNA levels and that of the protein levels of hTERT and c-myc (P<0.05). Conclusion Ouabain can down-regulate the mRNA and protein expressions of hTERT and c-myc, which may be one of the mechanisms of its induction of the apoptosis of Jurkat T lymphocyte leukemia cells .

Objective To analyze tissue-specific expression and polymorphic regulation of the UDP - glucuronosyltransferase (UGT) 1A gene in human tissue. Methods Forty colorectal cancer tissue. 20 normal colorectal tissue and 10 normal liver tissue were studied. UGT 1A transcriptions were investigated by exon-1 specific RT-PCR. UGT 1A proteins were studied using Western blotting analysis. Meanwhile catalytic activity of microsomes was evaluated using N-hydroxy-PhiP. Results (1) UGT 1A mRNA expressions were found to be significantly down-regulated in colorectal cancer tissue as compared with the surrounding normal tissues(P

Objective To compare the clinical effectiveness of incision-thread-drawing procedure and incision and drainage procedure in the treatment of perianal abscess.Methods Clinical data collected from 110 patients with perianal abscess were retrospectively analyzed.Forty-six cases were treated with incision-thread-drawing procedure(therapy group) and 64 with incision and drainage procedure(control group).Results Recurrence rate and incidence of anal fistula were 4%(2/46) and 4%(2/46) in therapy group,respectively,significantly lower than those in control group(19% and 23%,P

Objective To review the clinical characteristics of adult-onset Still's disease (AOSD) patients with special pulmonary complications.Methods Medical records of 506 AOSD inpatients,who were admitted in Peking Union Medical Hospital from October 1997 through March 2015,were retrospectively reviewed.The clinical features,biochemical parameters,radiology,pulmonary function tests,ultrasound,treatments and prognosis of AOSD patients with special pulmonary complications were analyzed.Results The incidence of special pulmonary complications in AOSD inpatients was 2.2％ (11/ 506),including 3 cases of interstitial lung disease and 8 cases of pulmonary artery hypertension.All 11 patients had common manifestations of AOSD,including fever (11/11),arthralgia/arthritis (11/11),rash (9/11),lymphadenopathy and/or splenomegaly (8/11),serositis (10/11),abnormal liver function (9/ 11),neutrophilia (11/11),and hyperferritinemia (11/11).The patients with interstitial lung disease had no or mild symptom,and were diagnosed by HRCT and pulmonary function test.The patients with pulmonary artery hypertension had various symptoms including dyspnea of exertion (8/8),cough (4/8),chest suppression (2/8),and chest pain (2/8).Four patients died from severe infection,including 3 cases of pulmonary artery hypertension and 1 case of interstitial lung disease.Conclusions Interstitial lung disease and pulmonary artery hypertension are life-threatening complications of AOSD patients.Earlier recognition and prompt treatment should be made to improve patient outcomes.

Based on M9 culture medium,the concentration of ingredients of culture medium was optimized for the fermentation of pNSR32/BL21(DE3),the engineering bacterial with spider silk protein,and lactose as an inducer.The condition of optimum culture medium was obtained for the expression of the high molecular weight recombinant spider silk protein by using orthogonal and individual factor experimental design.The result was showed that the optimum culture medium was consisted of 0.3% glycerol,3% yeast,0.75% tryptone,0.05%(NH_4)_2SO_4 and a little inorganic salt_.It was confirmed that the optimum culture medium will benefit the growth of bacterial and expression of recombinant spider silk protein.The production level of propose protein will attain at 20% of the total proteins in the fermentation.

A combination vaccine which consists of ≥2 immunogens is intended to provide protection against two or more diseases or against multiple serotypes of a single disease. The use of combination vaccines decrease the number of vaccine injections in the regular immunization schedules. However,the development of combination vaccines is more complicated than monovalent vaccines,preservatives and adjuvants used with one component may alter the potency of other components. Physical,chemical,and biological interactions between the components of combination vaccines must be considered to avoid detrimental effects on safety or efficacy. Therefore combination vaccines present unique challenges for product evaluation. This paper presents a review of research application status,the evaluation of effectiveness and safety,as well as development prospects on combination vaccines.