AIM:To observe the clinical characteristics of palm video keratitis and analyze the pathogeny. And to explore methods to cure and prevent it.
METHODS: A total of 591 patients were randomly selected. Firstly, the frequency of staring at palm video equipment were investigated and break-up time of tear film with slit-lamp were observed. Then the data were calculated and the clinical characteristics were described. Finally, the correlation between incidence rate and the two factors were analyzed by SPSS13. 0 respectively.
RESULTS:The incidence rate of palm video keratitis increased along with the frequency of staring at palm video equipment and decreased along with break- up time. There was significant relationship between incidence rate and the two factors respectively.
CONCLUSION:It is the most important pathogeny that staring at palm video equipment ahead and down for a long time which cause the change of tear film. That can reduce the frequency of using palm equipment and drop artificial tears to prevent and cure the disease.

Objective To investigate the clinical efficacy and safety of medroxyprogesterone acetate in the treatment of endometrial atypical hyperplasia patients.Methods 98 cases of endometrial atypical hyperplasia patients were randomly divided into study group and control group with 49 cases in each group, study group were treated with medroxyprogesterone acetate treatment, and control group were given estradiol valerate tablets treatment.Two groups of patients were treated for 3 ~6 menstrual cycles, and clinical curative effect of two groups was compared.Results After treatment, the PBAC score and endometrial thickness inspection results in study group and control group respectively decreased significantly compared with before treatment (P<0.05), and the above indexes in study group were significantly lower than control group (P<0.05).After treatment, the hemoglobin value in two groups at 1st, 2nd, 3rd month after treatment was significantly higher than before treatment (P<0.05), and hemoglobin values at 1st, 2nd month after treatment was significantly higher than control group (P<0.05).The effective rate of study group (95.92%) was significantly higher than that of control group (81.63%, P<0.05).The recurrence rate during six months was 4.08% in study group, 8.16% in control group, and the difference was not statistically significant.Conclusion The efficacy of medroxyprogesterone acetate is better than estradiol valerate tablets in the treatment of endometrial atypical hyperplasia patients, which is safe and effective.

Aged ; Back ; pathology ; CD57 Antigens ; metabolism ; Chondrosarcoma ; metabolism ; pathology ; Diagnosis, Differential ; Fibroma, Ossifying ; metabolism ; pathology ; Humans ; Male ; Phosphopyruvate Hydratase ; metabolism ; S100 Proteins ; metabolism ; Soft Tissue Neoplasms ; metabolism ; pathology

Angiofibroma ; metabolism ; pathology ; Biomarkers, Tumor ; metabolism ; Diagnosis, Differential ; Epithelioid Cells ; Extremities ; Fibroma ; metabolism ; pathology ; Fingers ; Hemangioblastoma ; metabolism ; pathology ; Hemangioendothelioma ; metabolism ; pathology ; Humans ; Rhabdomyosarcoma ; metabolism ; pathology ; Sclerosis ; Soft Tissue Neoplasms ; classification ; metabolism ; pathology

Antineoplastic Agents ; therapeutic use ; Benzamides ; therapeutic use ; Biopsy, Large-Core Needle ; Drug Delivery Systems ; Humans ; Imatinib Mesylate ; In Situ Hybridization, Fluorescence ; Piperazines ; therapeutic use ; Protein Kinase Inhibitors ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; Pyrimidines ; therapeutic use ; Soft Tissue Neoplasms ; classification ; diagnosis ; genetics ; pathology ; therapy

OBJECTIVETo investigate the effect of 8-aminolevulinic acid (ALA) photodynamic therapy (PDT) on the cell proliferation, apoptosis and collagen secretion in keloid fibroblasts and to provide the theoretical base for ALA-PDT treatment of keloids.

METHODSFibroblasts from keloid patients were cultured to the third generation in vitro and incubated in 0, 1, 3, 6, 9 mmol/L of δ-aminolevulinic acid for 3 h in the darkness. Then they were exposed to 635 nm wavelength red light ( 30 J/cm2 ) and continued incubation 24 h after irradiation. CCK-8 assay was used to detect proliferation inhibition rate of fibroblasts. The content of hydroxyproline was measured by colorimetric method. The expression of p-Akt and programmed cell death 4 ( PDCD4) were detected by Western blot.

RESULTSThe inhibition rate of keloid fibroblasts were respectively 0, (8.30 ± 1.01)%, (29.48 ± 3.27)%, (52.01 ± 5.34)%, (79.99 ± 5.85)% with the presence of difference concentrations (0, 1, 3, 6, 9 mmol/L) of ALA. The content of hydroxyproline were respectively (9.540 0 ± 0.352 42), (6.242 5 ± 0.224 85 ), (5.107 5 ± 0.534 88), (3.490 0 ± 0.623 48), (2.945 0 ± 0.514 10) μg/mg. The relative expression of p-Akt were respectively 1, 0.75 ± 0.12, 0.52 ± 0.14, 0.41 ± 0.18, 0.32 ± 0.09. The relative expression of PDCD4 were respectively 1, 1.18 ± 0.19, 1.51 ± 0.22, 0.15 ± 0.30, 2.44 ± 0.22. The difference was statistically significant when compared the group of 1, 3, 6, 9 mmol/L with 0 mmol/L (P < 0.05).

CONCLUSIONSIn concentration within the range of 1-9 mmol/L, ALA could inhibit the proliferation of fibroblasts significantly, promote fibroblasts apoptosis and reduce the content of hydroxyproline in a dose-dependent manner, indicating that 8-aminolevulinic acid photodynamic therapy may be a potential treatment for keloid.

Aminolevulinic Acid ; pharmacology ; Apoptosis ; drug effects ; Cell Culture Techniques ; Cell Proliferation ; drug effects ; Collagen ; secretion ; Fibroblasts ; cytology ; drug effects ; secretion ; Humans ; Keloid ; drug therapy ; pathology ; Light ; Photochemotherapy ; methods ; Photosensitizing Agents ; pharmacology

Objective To explore the neuroprotective effect of dexmedetomidine ( Dex) in rats ex-posed to focal cerebral ischemia/reperfusion ( I/R) induced by middle cerebral artery occlusion ( MCAO) and the possible mechansims.Methods Adult male Sprague-Dawley rats were subjected to MCAO for 90 min followed by reperfusion for 24 h and Dex ( 15μg? kg-1 ) was infused through the left femoral vein im-mediately after the onset of MCAO.The PI3K inhibitor LY294002 ( LY,10 mM,10 μl) or eNOS inhibitor L-NIO ( 1 mg? kg-1 ,10μl) was intracerebroventricular administered before ischemia using a microinjecton. The neurological deficit score,brain edema,cerebral infarct volume,and neuron survivals were evaluated after 24 h of reperfusion.The expression of p-Akt ( Ser473) and p-eNOS ( Ser1177) in the ischemic hemicere-brum was detected by Western Blot.Results Compared with I/R group,the neurological deficit score,cere-bral infarct volume,and the degree of brain edema were significantly reduced in the rats treated with Dex ((2.3±0.4) vs (3.9±0.6),(19.3±3.5)%vs (40.5±5.4)%,(61.8±8.1)%vs (76.3±8.5)%,all P<0.05)re-spectively,and the number of survival neuron in the hippocampal CA1 and cortex was significantly increased ((136.5±15.8)/mm vs (53.5±7.9)/mm,(253.8±26.4)/mm3vs (112.5±14.6)/mm3,all P<0.01) respec-tively.The neuroprotection in DEX group was significantly inhibited by LY and L-NIO ( P<0.05) .In addi-tion,the expression of p-Akt and p-eNOS in the ischemic hemicerebrum was markly reduced in LY group compared with that in DEX group ((3.94±0.45) vs (0.85±0.21),(2.14±0.25) vs (0.79±0.29),all P<0.01) ,while there was no significant difference in the expression of p-Akt in the ischemic hemicerebrum be-tween L-NIO group and DEX group ((3.76±0.33) vs (3.94±0.45), P>0.05).Conclusion Dex can reduce cerebral injury in rats exposed to focal I/R,which is mediated by the activation of PI3K/Akt-eNOS pathway.

Human genes typically contain multiple intron s, and in many cases the exons can be joined more than one way to generate multi ple mRNAs, encoding distinct protein isoforms. This process is called alternativ e splicing. The article summarized the human cytochrome P450 pre mRNA alternati v e splicing and their regulatory mechanism and impacts on biological functions.

On the model of myocardial damage induced by subcutaneous injection of isoproterenol (40mg/kg, twice) into rats, it was found that the myocardial calcium content and the level of myocardial angiotensin Ⅱ (AGT Ⅱ,ir) were significantly elevated, and that severe myocardial ischemic lesion occurred. The treatment with taurine (200mg/kg, twice) or captopril(1mg/kg, twice)antigonized the above effects of isoprotercnol dramatically and ameliorated the myocardial damage. The results suggest that taurine has inhibitary action on synthesis of heart AGT Ⅱ, and that this is one of the important mechanisms by which taurine protects heart from isoproterenol-induced myocardial injury.

Objective Ethanol, acid and alkali were used in the traditional Bererine extracting which required quite a few extracting time and polluted the environment. Water instead of ethanol was used. Microwave irradiation was chosen as the extracting method. Methods The best parameters, microwave power, extracting time, solid-liquid ratio were got in the experiment. According to the orthogonal experimental design, the optimum extracting condition was determined. Results Microwave irradiation, compared with the traditional extracting techniques, was with short extracting time. Conclusion The method was practicable and the product possessed the virtue of high purity, safe quality and free pollution.