Measurement of IgG subclass productions, activities of interleukin-6 (IL-6) and B cell differentia- tion faotors (BCDFs) in vitro were carried out on 37 children with IgG subclass deficiency. The frequency of defective IgG subclass productions induced by PWM is in order asfollows: IgG (7/9), IgG4 (6/11), IgGl (9/17) and IgG3 (2/13). Decreased CD4 oells from PBL, low leves of lymphocyte proliferative responses to PHA and SAC, and reduced activities of IL-6 and BCDFs were found in IgG subclass deficiency patinets. 12 out of 15 cases who had decreased IgG subclass production associated with a reduced activities of IL-6 or/and BCDFs; only 4 out of 15 cases who had normal amounts of IgG subclass production associated with such lymphokine defects. The results would suggest that IgG subclass deficiency may be related to the aberration of isotype switch in order along Ig heavy chain constant gene from 5' -3', and that a variety of abnormalities in T and B cell functions share the contribution to the pathogenesis of IgG subclass deficiency, in which T cell dysfunction may play a more important role.

Objective To investigate the common pathogens of endophthalmitis and their drug sensitivity .Methods 184 patients with endophthalmitis were selected .The aqueous humor or vitreous body was taken to inoculate in broth ,blood agar and potato dex‐trose agar medium for conducting bacterial and fungal culture .The K‐B method was adopted to conduct the drug sensitivity test .Re‐sults Among 184 cases ,9 cases were the mixed infection and 193 strains of bacteria were co‐cultured .Among them ,gram‐positive bacteria infection was most common ,108 strains were detected ,accounting for 55 .96% ,followed by gram‐negative bacteria ,63 strains were detected ,accounting for 32 .64% ,the detected fungi were less (22 strains) ,accounting for 11 .40% .The cultured gram‐positive bacteria were most sensitive to cefoperazone and cefuroxime both with the sensitivity of 87 .96% ,at the same time ,levo‐floxacin and cefazolin showed good sensitivity ,with the sensitivity of over 75% ,while the sensitivity to lomefloxacin was low ,only 50 .00% .Gram‐negative bacteria had highest sensitivity to lomefloxacin ,reaching 100 .00% ,in addition the sensitivity to ciprofloxa‐cin and ofloxacin also reached 87 .30% and 88 .89% ,while the sensitivity to cefuroxime ,ceftazidime and cefazolin was lower ,which were only 38 .10% ,39 .68% and 44 .44% respectively .Conclusion The infection types of pathogens should be comprehensively considered according to the causes of endophthalmitis occurrence .The combined medication should be selected during the empiric medication process after suffering from endophthalmitis .

Objective To explore the etiology,pathogenesis,diagnosis and treatment of functional evacuation disorder in gastric stump after subtotal gastrectomy. Methods 15 cases with functional evacuation disorder after sub-total gastrectomy were analyzed retrospectively. Results All 15 cases were recovered by conservational therapy. Con-dusion Functional delayed gastric emptying after subtotal gastrectomy is a functional disease and can be healed with comprehensive conservation therapy at all.

Pancreatic cancer (PC) is a common digestive tract tumor with high-degree malignancy,high mortality,and difficulty in diagnosis and treatment.Currently,comprehensive therapy becomes the standard and trend of tumor treatment.The treatment of PC has been developed from operation to the comprehensive therapy,including a variety of therapeutic methods.The progress of comprehensive treatment in PC was reviewed in this article.

Objective To explore the prevalence and risk factors of rheumatoid arthritis in the elderly population in Luohe District of Henan Province.Methods A total of 8 610 residents were recruited for a two-stage cluster sampling survey through a questionnaire of RA according to 2010 ACR/EULAR classification criteria.Physical examinations and blood tests were performed.Results The crude prevalence rate of rheumatoid arthritis was 0.76％ and 0.71％ for standardized prevalence in the middle-aged and elderly population in Luohe city.In the age distribution,of the 65-69 years old and 60-64 years old prevalence were 1.13％ and 0.84 ％,and 1.10 ％ and 0.77 ％ for standardized prevalence respectively.Sexual comparisons showed a significantly higher prevalence in females than in males (1.02 ％ vs.0.39 ％,x2 =10.513,P =0.001).The risk factors of RA were:sex (female),living floor (first floor),smoking and genetic factors,risk were 2.595 (95％CI:1.407-4.784),2.967 (95％CI:1.749-6.307) 1.682 (95％ CI:1.206-4.039) and 1.934 (95 ％ CI:1.286-4.130).Conclusion The prevalence of rheumatoid arthritis was 0.71％ in the middle-aged and elderly population in Luohe city.The risk factors involve women,smoking and hereditary factors

Objective To study the clinical effects of sequential therapy and standard triple therapy for Helicobacter pylori.Methods 120 cases with positive Helicobacter pylori were divided into A group and B group according to the number table method,60 cases in each group.A group received sequential therapy ( omeprazole+amoxicillin treatment after 5 days continue to omeprazole clarithromycin and metronidazole treatment for 5 days),B group received standard triple therapy ( omeprazole +amoxicillin +clarithromycin ) , eradication rate of Helicobacter pylori,the total efficiency of ulcer healing and adverse reaction of the two groups were compared.Results The eradication rate of Helicobacter pylori of group A was 91.7%(55/60),which was significantly higher than 71.7%(43/60) of group B (χ2 =11.915,P=0.023);the ulcer healing and the total effective rate of group A was 96.7%(58/60),which was significantly higher than 88.3%(53/60) of group B,the difference was statistically significant (χ2 =9.915,P=0.038);there was no statistically significant difference between the two groups in adverse reaction (χ2 =4.735,P=0.072).Conclusion Sequential therapy has a better effect in treatment Helicobacter pylori than the standard triple therapy, which can significantly improve the eradication of Helicobacter pylori, with no serious adverse reactions.

The influenza A is an acute respiratory infection persistently threatening human health and social stability, and has caused high morbidity and mortality. The development of novel anti-influenza drugs based on new targets is very significant because of high mutation and drug resistance of influenza virus. The nucleoprotein of influenza A virus identified high conservation, provides cross immune protection as a potential target of anti-influenza drugs and reports on relevant studies have been published at home and a- board. Herbal drug as a traditional Chinese medicine shows the distinct advantages in the aspect of prevention and treatment of influenza A. This paper analyzes the structure and function of influenza a virus, and reviews the advances in the research on anti-influenza targets based on the nucleoprotein of the influenza A virus.
Animals ; Drug Discovery ; methods ; Humans ; Influenza A virus ; drug effects ; metabolism ; physiology ; Influenza, Human ; drug therapy ; Molecular Targeted Therapy ; methods ; Nucleoproteins ; chemistry ; metabolism ; Virus Replication ; drug effects

[Objective]To study the possibility of differentiation of rabbit marrow mesenchymal stem cells(MSCs)into endothelial cells in vitro.[Method]BMSCs of rabbits were obtained by using gradient centrifuge method.The adhesive cells were preserved to passage culture to get pure MSCs.MSCs in the second generation were used.After induction with vascular endothelial growth factor(VEGF),microscopic exarm,cytoimmunofluorescent staining confirmd the trial group cells becomes to endothelial cells.W-P corpuscles which was the symbol of endothelial cells were observed by transmission electron microscope.[Result]The differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy.The cytoimmunofluore-scent staining showed that the trial group cells were induced to endothelial cells.W-P corpuscles could be seen under transmission electron microscope.[Conclusion]The adult rabbit BMSCs can present features of endothelial cells in certain induction medium,they are ideal donor cells of tissue engineering vascularization.

[Objective] To evaluate the value of Fulcrum-bending radiograph and new treatment strategy to the scoliosis with Chiari malformation and/or syringomyelia,and to analyze the effect of operation.[Methods]Eighteen cases suffering from scoliosis with Chiari malformation and/or syringomyelia were divided into 2 groups for surgical treatment.(1)Group 1:six cases had scoliosis with Chiari malformation and/or syringomyelia without obvious neurological impairment.Their scoliosis was corrected with anteroposterior instrumentation afer one week of Halo's traction.(2)Group 2:another 12 patients having similar scoliosis underwent posterior orthopedic operation according to the result of preoperative suboccipital Fulcrum-bending radiograph.[Results]In 6 patients who underwent opration after traction,the average frontal correction rate was 61%.In another 6 cases having soft scoliosis with only posterior operation,the average frontal correction rate was 60%,and the result of opration came close to the Fulcrum-bending radiograph's.But in 6 cases having rigid scoliosis with only posterior operation,the average frontal correction rate was 52%.One patient had nervous lesion and gradually recovered.There existed excessive rectification.[Conclusion]Patients with soft scoliosis with Chiari malformation and/or syringomyelia without obvious neurological impairment can be operated on according to Fulcrum-bending radiograph without preoperative traction,and the therapeutic effect is similar to classic operative result.

The under-water exercise is a specific sports.Because of the different of pressure,buoyancy,resistance and heat conductivity between water and land,The effect of under-water exercise on body's physiological function was different from the land.It has been evaluate in this paper that the characteristic of physiological function and regularity of energy metabolism in the under-water exercise