Objective To study the clinical value of the microbe-positive blood culture substance directly detected with Automated Microbiology System,as well as the coincidence rate between the direct method and the regular one(The puremicrobe were detected by WalkAway 40 after reiuoculated,and applied to antibiotic susccptibility tests).Methods To mix the positive blood culture liquid with the special inoculum liquid,then inoculate with the special identify panel to take bacterium identifying and antibiotic susceptibility testing on the system directly.At last,to contrast the result to the regular one.Results The coincidence rate between 74 samples of the positive blood culture substance on the system directly and the regular method was more than 94.4%.And the coincidence rate of antibiotic susceptibility test between the direct method and the regular one was more than 95%.And the results of the direct method could be acquired 24h earlier than those of the regular one.Conclusion The direct antibiotic susceptibility test method reduces the testing time,and get more time for bacteria treating.Meanwhile,the coincidence rate between the regular method and the direct one is high.

Aged ; Alkaline Phosphatase ; blood ; Calcium Gluconate ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Estradiol ; blood ; Female ; Humans ; Middle Aged ; Osteoporosis, Postmenopausal ; drug therapy ; Phytotherapy

Objective To evaluate the chemoprevention and therapy of tea polyphenol of colorectal cancer in colon cancer rats and cells.Methods Such database as CBM,CNKI,VIP,Wanfang,PubMed, Medline,EMBase,Cochrane Library were searched for collecting the papers including using tea polyphenol as a therapy and chemoprevention of colorectal cancer in colorectal cancer rats and colorectal cells,from their estab-lishment to June 1 ,201 4,and the references of those related papers were also searched by hand.After studying selection,assessment and data extraction were conducted by two reviewers.RevMan5.2 software was used for Meta-analyze.Results Seventeen studies were included.Three hundred and five rats and 5 colorectal cell lines were used to the research,they were divided into the study group (received the tea treatment)and the control group (not received the tea treatment).The results of meta-analyses showed that:① Aberrant crypt foci (ACF):standardized mean difference SMD =-3.69 (95%CI 为 -5.85 ~-1 .54,Z =3.40,P =0.000 8),which indicated that tea polyphenol could reduce the number of ACF.② The average number of tumors per rat:SMD =-0.83 (95%CI:-1 .1 4 ~-0.51 ,Z =5.1 8,P =0.000 01 ),which indicated that tea polyphenol could reduce the average number of tumors per rat.③ Cell cycle G1 phase:SMD =1 .85(95%CI:0.03 ~3.66,Z =1 .99,P =0.05 ),which indicated that tea polyphenol could increase the cell in G1 phase.④ Cell cycle S phase:SMD =-2.64(95%CI:-4.38 ~-0.90,Z =2.98,P =0.003),which in-dicated that tea polyphenol could decrease colon cancer cell in S phase.Conclusion Tea polyphenol has a positive effect on colon cancer cell and colon cancer rats.

BACKGROUND:Basic fibroblast growth factor can promote angiogenesis and connective tissue regeneration, but it can be degraded rapidly in vivo. Fibrin glue as a carrier can play a slow-release effect against the rapid degradation of basic fibroblast growth factor, so as to better play the biological role of basic fibroblast growth factor. But at present, the concrete application of them is stil in the research stage. OBJECTIVE:To review the research progress of the application of basic fibroblast growth factor and fibrin glue in orthopedics. METHODS:The recent literature concerning the application of basic fibroblast growth factor and fibrin glue in orthopedics was extensively reviewed by the first author through a computer-based retrieval of CNKI and Medline databases (2000-2014). RESULTS AND CONCLUSION:Finaly 64 articles were included in result analysis. Basic fibroblast growth factor can promote wound healing and tissue repair with the fibrin glue used as a carrier, but most studies are stil in the experimental stage and its clinical application needs further study.

Objective To assess the in-hospital prognostic value of NT-proBNP in patients with acute coronary syndrome (ACS) and its relation to the severity and prognosis of coronary artery disease .Methods Totally 132 consecutive patients with ACS in Affiliated Hospital of Nantong University from January 2013 to December 2014 were collected in this study ,in which 64 pa-tients with unstable angina(UA) ,46 patients with non-ST segment elevation myocardial infarction(NSTEMI) ,and 22 patients with ST segment elevation myocardial infarction(STEMI) .Patients were divided into two high NT-proBNP group(NT-proBNP ≥ 474 pg/mL) and low NT-proBNP group(NT-proBNP < 474 pg/mL ) .Ejection fraction were detected and compared between the two groups ,coronary artery disease were detected by coronary angiography .cTnI ,CK-MB ,CRP and NT-proBNP were detected by sero-logical ,and the average length of hospital stay and heart failure ,cardiac shock incidence were calculated .Results There was a sig-nificant negative correlation between NT-proBNP and ejection fraction .Incidence of heart failure and duration of hospital stay in high NT-proBNP group were significantly higher than in low NT-proBNP group ;The number of coronary vessels affected ,severity of stenosis and proximal left anterior descending artery(LAD) disease in high NT-proBNP group were higher than in low NT-proB-NP group ;TIMI flow grade was significantly lower in high NT-proBNP group than in low NT-proBNP group ,the difference was statistically significant(P< 0 .05) .Conclusion NT-proBNP is a valuable marker for predicting prognosis and severity of coronary artery disease in patients with acute coronary syndrome .

OBJECTIVE To investigate the distribution and drug resistance of Pseudomonas aeruginosa.METHODS All specimens isolated and cultured from patients in our hospital were identified by using the automatic microorganism analyzer WalkAway-40,Dade Behring made in America,and bacteria′s drug susceptibility test and identifications were performed on strains using NC21 Microscan Panel.RESULTS From sixty-two strains of P.aeruginosa 36 strains were isolated of sputum.The resistance rate to the third generation of cephalosporins cefotaxime and ceftriaxone was 55.6% and 47.5%,respectively.The resistance rate to the other ?-lactamases antibiotics such as ceftazidime was 7.5%,piperacillin/tazobactam 11.0%,cefepime 14.8% and penicillin was 30.0%,the ratio of resistance for imipenem was 20.0%,the lowest one was amikacin(4.6%).CONCLUSIONS P.aeruginosa is one of the main bacteria in the lower respiratory tract infection.The drug-resistant mechanism of P.aeruginosa is very complex,including multidrug resistance characteristics,and it is originally resistant to several antibiotics.To avoid being produced ?-lactamases and result in resisting drug widely,the antibiotics should be selected according to low drug-resistant rate and taking into account sufficiently its drug resistance mechanism in the treatment of P.aeruginosa infection.

OBJECTIVE:To promote the application of Personal data assistant(PDA)in the management and service of clinical medical information.METHODS:The application of PDA in medical fields and the chief function,the connotation of in_ formation service and whom the service aimed at were introduced.RESULTS:PDA was widely used in overseas medical infor-mation management and service for its distinct virtues of portability,speedy and accurate information service.CONCLUSION:PDA is an important vehicle in helping medicinal professionals’access to information.

Humans ; Lumbar Vertebrae ; surgery ; Spinal Diseases ; surgery ; Spinal Fusion

Objective To investigate the clinical features and prognosis of replantation of fingertips distal to the middle nail.Methods Between August 2001 and August 2005,16 fingertips amputated in the Ishikawa zoneⅠwere replanted.We anastomosed only the digital arteries,and adopted postoperative bloodletting.Results Fourteen fingertips survived,and two failed.The survival rate was 87.5%.Of the 14 successfully replanted fin- gertips,12 were followed up from 3 to 12 months.The length and appearance of the fingertips recovered and good joint motion were maintained.Their two-point discriminations ranged from 3 to 6 mm.Conclusion Fingertip replantation is an optimal treatment for fingertip amputation distal to the middle nail to regain satisfactory appearance and function of the digits with a high survival rate,but severe crush injury to the amputated part and high cost should be taken into consideration.

Basic research program is essential to the investigation of the pathogenesis of ocular diseases and the development of novel strategies for the prevention and treatment for these diseases. With increasing support of research grants at various levels, basic research in ophthalmology has gained great achievement in China in recent years. A number of studies have recently been published in well known peer-review international journals and won the State Scientific and Technological Progress Awards. However, we have to keep it in mind that basic research in ophthalmology should be improved qualitatively meanwhile, the imbalance in basic study among different areas needs to be resolved in the near future.