Pulmonary artery hypertension (PAH)is a common clinical syndrome.The over-proliferation of pulmonary arterial smooth muscle cells (PASMCs)is a hallmark of pulmonary vascular remodeling which is a critical and fundamental pathogenesis in the development of a variety of pulmonary artery hypertension.Here,we review the advances in studies on signaling transduction pathways mediating the proliferation of PASMCs and also introduce the existing approaches in inhibiting their proliferation and relevant research advances.

Objective To study the effects of iodine on the proliferation activity of fibroblasts at different doses. Methods The cultured fibroblasts were treated with different dose iodine(100 ?g/L, 500 ?g/L, 1 000 ?g/L, 3 000 ?g/L, 5 000 ?g/L, 7 000 ?g/L, 9 000 ?g/L, 11 000 ?g/L)for 24 h and observe the morphology of fibroblast. MTT colorimetry was used to detect the fibroblast proliferation activity. Results Iodine could significantly increase the fibroblast proliferation activity at certain range of concentration (7 000 ?g/L). The proliferation activity was highest from 5 000 ?g/L to 7 000 ?g/L, the differences were significant compared with the iodine free control. Conclusion Iodine, the exposure level is 7 000 ?g/L.

Pressure sores result from many risk factors, which influence each other. In this paper, various aspects of the development of pressure sores in recent years were summarized, consisted of the main risk factors, pathogenesis, treatment and care methods. To control pressure sores should focus on prevention, and reflect the integrity and the target population.

Objective To observe the effects of fluoride of different dose on the proliferation of the cultured human umbilical vein endothelial cells(HUVEC). Methods The cells were treated with NaF at different doses. Cell counting, MTT colorimetry, flow cytometry (FCM) and immunohistochemistry were used to detect the endothelial cells activities. Results Compared with the fluoride free control, the positive rate of PCNA and Ki-67 were higher as NaF concentration was at 120-240 ?mol/L and were highest at 240 ?mol/L, the same was seen in the values of A and PI, as NaF concentration was at 600-960 ?mol/L,these indexes decreased. Conclusion In vitro, the low concentration of fluoride can promote the proliferative activity of HUVEC, whereas the high concentration can inhibit it.

BACKGROUND:Measurement of cardiac troponin plays an important role in diagnosis of myocardial infarction.OBJECTIVE:To label the rabbit cardiac troponin C(cTnC) by a fluorescent probe 5-iodoaccetamidofluorescein(5-IAF),and to observe whether the 5-IAF can be used to study the interaction between cTnC and other contractile regulatory proteins.DESIGN,TIME AND SETTING:A randomized control experiment was performed at Department of Human Anatomy,Guangzhou Medical College,from January 2002 to December 2005.MATERIAL:Adult rabbits were provided by Experimental Animal Center of Guangzhou Medical College.METHODS:The rabbit cTnC DNA fragment was prepared with RT-PCR method.This gene fragment was cloned to pET expression vector by gene recombination technology.The site-directed mutagenesis were used to produce a mutant containing single cysteine at position 84 by replacing Cys35 with Ser,cTnC(C35S).The cTnC(C35S) was labeled by 5-IAF and 2-(4'-(iodoacetamido) anilino) naphthalene-6sulfonic acid(IAANS),Respectively.And then,the fluorescence emission(steady-state and time-resolved) was performed.MAIN OUTCOME MEASURE:The fluorescence properties of 5-IAF-labeled cTnC(C35S) and IAANS-labeled cTnC(C35S).RESULTS:The excitation of apo-cTnC(C35S)IAF was performed at 491 nm,and the emission peak was at 520 nm.Saturation of cTnC(C35S)IAF with Mg led to a 35% decrease in fluorescence intensity.Another 35% decrease with a 3 nm-blue shift was seen as the protein was saturated with Ca.The two-phase transitions of fluorescence emission from IAANS-labeled cTnC in response to Mg and Ca did not appear in fluorescence emission of 5-IAF-labeled cTnC.However,the Ca-induced conformational change in cTnC remained unchanged no matter which probe was used.Ca titration experiments showed that binding parameters derived from the fluorescence emission of the two probes were comparable.CONCLUSION:5-IAF is an appropriate probe that can be used to study the interaction between cardiac troponin C and other contractile regulatory proteins.

Objective To evaluate the effects of exercise therapy in children with congenital heart disease. Methods To search databases such as PubMed, Medline, Science Direct, Embase, Cochrane Library, China National Knowledge Infrastructure, WanFang, VIP, China Biology Medicine for all the randomized controlled trials. After evaluating the quality of each article, the software of RevMan 5.3 were used to analyze. Results A total of 10 randomized controlled trials were included. Compared with the routine nursing, meta-analysis showed that exercise therapy can improve the peak oxygen uptake(MD=4.82,95%CI 2.25-7.39, P=0.0002) and the postoperative compliance (RR=2.84, 95%CI 1.75-4.63, P<0.01), shorten the postoperative hospital time(MD=- 4.41,95% CI - 6.15-- 2.68, P <0.01). Conclusions Exercise therapy can improve the pulmonary function and quality of life on children with congenital heart disease, shorten the postoperative hospital time, increasing the postoperative compliance. However, there were few research on present, so we need a large sample randomized controlled trials of long time to confirm the effects of exercise therapy.

Esophageal collision tumor is an extremely rare tumor which defined as the concrescence of two distinct primary neoplasms.The pathobiological mechanism of collision tumors is yet to be understood.Clinical symptoms,endoscopic examination and imaging are all lack of specificity.Diagnosing a collision tumor prior to surgery is difficult.Careful pathological examination is crucial for accurately diagnosing the neoplasms in a collision tumor and ensuring appropriate management and a favorable prognosis.Esophageal collision tumors have been increasingly reported in recent years.With the aim of improving the knowledge level of esophageal collision tumor,the clinical and pathological features of this tumor is needed to be summarized.

Objective To examine the effect of labor analgesia with ropivacaine on maternal serum prolactin, time of first eolostrum production and the rate of abundant lactation. Methods A total of 124 women of vaginal delivery were randomly divided into labor analgesia group (n = 75 ) and control group (n =49). Labor analgesia group received ropivacaine by patient-controlled epidural analgesia. Three ml ropivacaine (0.125% ) was injected through an epidural catheter and another 12 ml ropivacaine was injected 5 min later if there were no total spinal anesthesia. The block level of analgesia was controlled to be below T10 level. Then 5 ml (0.104 mg/min) ropivacaine per hour was continuously pumped till full dilation of ostium of the uterus. The control group consisted of women of normal spontaneous delivery with no pain relieving measure. The prolactin levels of antepartum, postpartum 0 h, 2 h, 6 h, 12 h and 24 h were determined by microparticle chemoluminescence. Starting time of lactation, the feeding times in 24 hours,the rate of abundant lactation, and neonatal weight 24 hours after delivery were recorded. Results ( 1 ) The serum prolactin of both groups increased instantly after delivery, reached a peak 2 hours after delivery and kept high levels 24 hours after delivery. (2)The prolactin levels of labor analgesia group [(19. 5±8.4)nmol/L and ( 14. 5 ± 5.6 ) nmol/L] were lower than those of control group [( 22.6±7. 2 ) nmol/L and ( 16. 9 ± 5.7 ) nmol/L] 2 and 24 hours after delivery ( P < 0. 05 ). ( 3 ) In labor analgesia group the starting time of lactation was within 24 hours after delivery in 73 cases (97%), lactation amount was abundant within 48 hours in 55 cases (73%) and newborn weight reduction in the first day after delivery was(57 ±42)g. In control group the starting time of lactation was within 24 hours after delivery in 45 cases (92%),lactation amount was abundant within 48 hours in 28 cases (57%) and newborn weight reduction in the first day after delivery was( 62±40)g. There were no differences between the two groups in the starting time of lactation, the rate of abundant lactation, and newborn weight reduction ( P > 0. 05 ). Conclusions ( 1 )Epidural anesthesia with ropivacaine might affect the secretion of prolactin, while the starting time and amount of lactation may be affected by other factors. (2) Prolactin increases after delivery, reaches a peak 2 hours after delivery and maintains high levels 2.4 hours after delivery, which contented necessary for lactation.

Objective To investigate the preventive effects of aspirin on liver metastases of colorectal cancer in mice and study the mechanisms.Methods Twenty BALB/c mice were divided into the control group and the experimental group according to the random number table with 10 mice in each group.Mice in the control group were fed with saline each day at a concentration of 0.2 mL/d for 60 days,while mice in the aspirin group were fed with aspirin each day at a concentration of 30 μg/(g · d) for 60 days.Then C26 colon cancer cells were injected into the spleen and then the spleen was cut to establish mice model of colon cancer liver metastasis.The C26 colon cancer cells were divided into 2 groups.C26 colon cancer cells in the control group remained untreated,and C26 colon cancer cells in the experimental group were treated with aspirin at a concentration of 10 mmol/L for 24 hours.The scratches and transwell assays were conducted to observe the effects of aspirin on the invasion and metastasis of C26 colon cancer cells.The expressions of epithelial-mesenchymal transition (EMT)-related genes were detected using RT-PCR and Western blot.All data were analyzed using the Student t test.The survival curve was drawn by Kaplan-Meier method,and the survival analysis was done by Log-rank test.Results The numbers and weights of hepatic metastatic tumors were 4.8 ± 1.9 and (504 ± 107) mg in the control group and 2.6 ± 1.6 and (362 ± 67) mg in the experimental group,with significant difference between the 2 groups (t =2.840,3.584,P ＜ 0.05).The 1-month survival rate was 80％ in the experimental group,which was significantly higher than 40％ of the control group (x2=4.418,P ＜ 0.05).The results of pathological examination showed that tumor cell heteromorphism was reduced by aspirin.The results of scratches experiment showed an obvious migration of C26 colon cancer cells in the control group at 24 hours later,while no C26 colon cancer cells migrated in the experimental group.The numbers of C26 colon cancer cells penetrated the Watrige were 253 ± 21 in the control group and 148 ± 13 in the experimental group,with significant difference between the 2 groups (t =5.101,P ＜0.05).The relative mRNA expression of the E-cadherin and the Vimentin were 0.002 ±0.001 and 1.005 ±0.286 in the control group and 0.005 ± 0.001 and 0.270 ± 0.168 in the experimental group,with significant difference between the 2 groups (t =-4.606,4.942,P ＜ 0.05).The relative protein expressions of the E-cadherin and the Vimentin were 0.473 ±0.179 and 0.787 ± 0.118 in the control group and 1.585 ± 0.410 and 0.280 ± 0.133 in the experimental group,with significant difference between the 2 groups (t =-5.542,6.355,P ＜ 0.05).Conclusion Aspirin inhibits liver metastasis of colon cancer and promote the survival ratio of mice.Aspirin can up-regulate the expression of E-cadherin and down-regulate the expression of Vimentin,which inhibits EMT and reduces the invasion and metastasis of tumor cells.