1.Comparison of medical imaging technique professionals' education and working system between Chinese mainland and Taiwan region
Liangcheng ZENG ; Xuehua YAO ; Lijian GUO ; Yurong HONG ; Jialong CAO ; Lanzhu HUANG ; Shenghe WENG
Chinese Journal of Medical Education Research 2012;11(5):448-453
There are great differences in the education and working system of certain professionals between Chinese mainland and Taiwan region due to the differeuces in the political system.In this essay,we try to compare the differences in the education level,objective and course offering of the medical imaging technique major and the differences in the professional qualification examination system and the working system between Chinese Mainland and Taiwan Region.We also probe into the possible fields for both sides to learn from each other and to provide references for cooperative school - running,professionals' interchange and working system of medical imaging technique majors of both sides.
2.Rapid discovery of a novel"green"and natural GST inhibitor for sensitizing hepatocellular carcinoma to Cisplatin by visual screening strategy
Mao LINXI ; Qin YAN ; Fan JIALONG ; Yang WEI ; Li BIN ; Cao LIANG ; Yuan LIQIN ; Wang MENGYUN ; Liu BIN ; Wang WEI
Journal of Pharmaceutical Analysis 2024;14(5):693-706
Over-expression of glutathione S-transferase(GST)can promote Cisplatin resistance in hepatocellular carcinoma(HCC)treatment.Hence,inhibiting GST is an attractive strategy to improve Cisplatin sensi-tivity in HCC therapy.Although several synthesized GST inhibitors have been developed,the side effects and narrow spectrum for anticancer seriously limit their clinical application.Considering the abundance of natural compounds with anticancer activity,this study developed a rapid fluorescence technique to screen"green"natural GST inhibitors with high specificity.The fluorescence assay demonstrated that schisanlactone B(hereafter abbreviated as C1)isolated from Xue tong significantly down-regulated GST levels in Cisplatin-resistant HCC cells in vitro and in vivo.Importantly,C1 can selectively kill HCC cells from normal liver cells,effectively improving the therapeutic effect of Cisplatin on HCC mice by down-regulating GST expression.Considering the high GST levels in HCC patients,this compound demon-strated the high potential for sensitizing HCC therapy in clinical practice by down-regulating GST levels.
3.Effect of dopamine on the activity of matrix metalloproteinases and degradation of dentin collagen
Qiangjian XU ; Quanli LI ; Jialong CHEN ; Weibo ZHANG ; Xiaoting WU ; Ying CAO
Chinese Journal of Stomatology 2015;50(3):186-189
Objective To investigate the inhibition effect of dopamine on the activity of matrix metalloproteinases(MMP) and the effect of dopamine on degradation of dentin collagen for its potential use in caries treatment and dentin adhesive.Methods In the experiment of MMP activity test,2.0 g/L dopamine+ 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the experimental group,and deionized water+ 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the negative control group,and 2% chlorhexidine + 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the positive control group,and the mixture volume ratio of the two ingredients in every group was 1∶9.After 15 minutes,the enzyme activity of each sample was tested by MMP activity colerimetric quantitative detection kits,and the test was repeated 5 times in each group.In the experiment of collagen degradation,the dentin slices were demineralized with 37% phosphoric acid for 1 min.In sequence,2 dentin slices were used to observe the morphology,and the remaining 30 dentine slices were randomly divided into three groups(n=10) according to random number table:the negative control ones were stored in 100 μl deionized water and 900 μl collagenase(7 days,37 ℃),the positive control ones were stored in 100 μl chlorhexidine and 900 μl collagenase(7 days,37 ℃) and the experimental specimens were stored in 100 μl dopamine and 900 μl collagenase(7 days,37 ℃).The degraded collagen was investigated by assaying hydroxyproline.The framework of collagen was evaluated with field emission scanning electron microscope(FE-SEM).Results The statistical results of completely random design ANOVA showed that the MMP activity and the amount of degraded collagen of the negative control group [(0.089±0.011) μmol · min-1· mg-1 and (2 837±201) μg/cm2] were significantly higher than those of the positive control group[(0.038± 0.006) μmol · min-1 · mg-1 and (1 288± 172) μg/cm2] and the experimental group[(0.030±0.009) μmol· min-1· mg-1 and (1 389±255) μg/cm2](P<0.05).SEM observation indicated that the structural integrity of the collagen network on dentin still existed in experiment samples and positive control groups,however,collagen fibrils were destructed and the structural integrity disappeared in the negative control groups.Conclusions Dopamine may inhibit MMP activity and reduce the amount of degraded collagen.