Objective To analyze the curative effect of acupoint catgut-embedding therapy for PCOS patients of obesity.Methods 60 PCOS patients are randomly divided into an experimental group (acupoint catgut embedding group)and a control group (acupuncture group),with 30 cases in each group.Observe such indexes of each patient as weight,lumbar,BMI and menses before and after treatment.Results The two methods are significantly effective to reduce weight and improve the menstrual disorder,with no statistically significant difference(P＞0.05).Conclusion Acupoint catgut-embedding therapy is an effective method to treat PCOS of obesity.

The blood brain barrier(BBB) is a barrier system between the blood and brain tissue,which can maintain relatively stable of internal environment in the central nervous system (CNS).Cerebral hypoxiaischemia and other conditions can lead to the change of the permeability of BBB.This paper reviews the latest research for the structural basis and function of BBB,and for the scope,time and mechanism of the change of BBB structure and function during hypoxic-ischemic brain injury.This theory in the regulation of BBB may play an important role in the treatment of hypoxic-ischemic brain injury and underlying diseases of CNS.

Paeoniflorin in antiaging Preparation was qualitatively identified with chemical and TLC methods and quantitatively determined with HPLC. The results showed both tne chemical and TLC method gave fast and accurate results and HPLC was an excellent for quantitative determination with an average recovery of 100.72% and coefficient of variation 1.22%, which is suitable for the quality control of the preparation.

Objective To investigate the relationship between genetic polymorphism in glutathione S-transferases T1, M1 and P1 and susceptibility to reflux esophagitis. Methods Genomic DNA was isolated from peripheral blood from the patients with reflux esophagitis (n=109), cases with negative endoscopy reflux disease (NERD) (n=97) and controls (n=97). Genes GSTM1 and GSTT1 were detected by multiplex PCR. A A→G polymorphism of codon 104 of the gene GSTP1 was detected using PCR-based restriction fragment length polymorphisms ( PCR-RFLP ) with Alw26I. Results The frequency of variant GSTP1 genotypes (*A/*B and*B/*B) was found to be 40.4% among the patients with reflux esophagitis compared with 24.7% among cases with gastroesophageal reflux without esophagitis and 21.6% among controls (P

Objective In order to research the operative method for preservation of healthy lung in the middle lobe carcinoma.Methods From June,1994 to August,1999,16 cases lobectomy of middle lobe cancer were performed.12 of 16 cases were received middle lobectomy with bronchial wedge-shaped resection,6 of which were followed by partial pulmonary artery resection,2 of which,with pulmonary artery sleeve resection,3 of 16 were cured by the middle lobe sleeve lobectomy,one of which was done with combined partial pulmonary artery resection.One of 16 received both middle and lower lobectomy,simultaneously,completed upper pulmonary vein and lower pulmonary vein reconstruction.Results 16 cases operation were all successful.The survival rate in 1 and 3 years were 88% and 50%,respectively.Conclusion Bronchovascular resection and reconstruction applied to treat the middle lobe cancer not only could maximal remove the focal lesion,but also reserve the healthy lung fully,and expanded the surgical indication.

Objective To investigate the clinical effects of reconstruction by using the free iliac flap with nerve anastomosis to repair the composite tissue defects of heel.Methods From March 2009 to August 2012,five cases with the composite tissue defects of heel,including 2 cases with the bottom defects and 3 cases with the tibial defects of heel,were repaired by the free iliac flap,and the feeling of the heel were reconstructed by nerve anastomosis of the flap.The ventral wound were sutured simply.Results All iliac flaps survived and the wound healed in one stage.Patients were followed up from 6 months to 12 months.Appearance and function recovered and the feeling recovered from S2-S3.The foots walked freely.The skin of iliac abdomen were scarred and lead to hyperpigmentation.Conclusion Using the free iliac flap with nerve anastomosis could repair the bone and soft tissue defects of heel and reconstruct the sensation of warmth and sense of pain of the heel.With the characteristics of hidden wound and little damage,the clinical effects was satisfied.

Objective To study the roles of dengue (DEN) virus specific T cells in the pathogenesis of DEN virus infection. Methods 2D42 cells, DEN virus specific CD8 + cell clones, were employed to investigate their in vivo function in DEN virus infection using an animal model. HepG2 was implanted into mice with severe combined immunodeficiency disease (HepG2-SCID) for the establishment of HepG2-SCID model. The animals were divided into 3 groups: Group A: HepG2-SCID mice were inoculated with 2D42 cells and then infected with DEN virus type 2 (DEN2) intraperitoneally; Group B: HepG2-SCID mice were inoculated with normal mouse thymuscytes (NMT) and then intraperitoneally infected with DEN2; Group C: HepG2-SCID mice were intraperitoneally infected with DEN2 alone. The mortality, viremia, and frequency of histopathological changes in the major organs of mice in the three groups were observed after infection. Results After inoculation of 2D42 cells, 80% infected mice showed severe clinical signs and died at the average 12.8 d after infection. The others only had transient manifestations, and then recovered from the disease and survived for more than 3 months. In contrast, after inoculation of NMT and /or DEN2 alone, 100% mortality rate was noted in these two groups. High viremia and frequency of histopathological changes in the major organs were observed in the mice in groups A and B. Conclusion Our data support both protective and pathogenic roles for DEN-specific CD8 + T cells in DEN virus infection.

Objective To investigate the effectiveness and the surgical experience of bronchoplasty and pulmonary artery reconstruction in the treatment of lung cancer of the left pulmonary artery affected. Methods From January, 1990 to December, 2004, 16 patients with lung cancer of the left pulmonary artery affected underwent bronchoplasty and pulmonary artery reconstruction. According to TNM classification, 16 patients were in stage ⅢA. The surgical procedures included sleeve resection of bronchus in 16 cases, sleeve resection of pulmonary artery in 11 and wedge resection of pulmonary artery in 5. Results The overall 1, 3 and 5-year survival rates were 75.0 %, 45.5 %, and 33.3 % respectively. Conclusion The results suggest that brenchoplasty and pulmonary artery reconstruction for the patients with lung cancer of pulmonary artery affected is an effective surgical technique. This method extends the surgery indications to patients with poor lung function and senility by reducing the ratio of pneumonectomy and improving postoperative quality of life. Therefore, it is clinically valuable.

Objective To describe our follow-up results of interventional management in 20 patients with subclavian artery stenosis. Methods This study involved in 20 symptomatic patients with an average age of 63.4 years,a mean stenose severity of 86.2% and a mean systolic arterial pressure difference of 116 mm?Hg between both upper limbs. Percutaneous transluminal angioplasty (PTA) in the first 8 patients and stenting in the last 12 patients were performed via a right femoral or radial artery. Results Both PTA and stenting were successfully archived in all patients with normalization of radial pulses and a mean systolic arterial pressure difference of 24 mm?Hg between both upper limbs. No major complications occurred. In the follow-up for an average of 18.5 months,19 patients were free of adverse events except one with symptom recurrence. Conclusion As effective,simple and safe procedures,PTA and stenting can be used as a first-line treatment modality for the symptomatic patients with subclavian artery stenosis.

Objective To explore the application and management of deep hypothermic extracorporeal circulation (DHECC) in surgical procedures. Methods From Dec. 1997 to Dec. 2007, DHECC was applied in 54 patients suffered from great vessel diseases (34/54), complex congenital heart disease (10/54), giant basilar artery aneurysm (5/54), and abdominal tumor with tumor embolus intruding into right atrium or inferior vena cava (5/54) in the General Hospital of PLA. Extracorporeal circulation was performed with arterial perfusion by ascending aorta (n=25), axillary artery (n=18) and femoral artery (n=11), and venous drainage by superior vena cava and inferior vena cava (n=21), right atrium (n=25), and femoral vein (n=8). Clinical data were analyzed retrospectively including extracorporeal circulation (ECC) time, aortic clamping time, deep hypothermic circulation time, the lowest temperature, and the use of ultrafiltration. Results The ECC time was 63-414 (178.55?74.42)min. Aortic clamping time was 16-259 (123.39?52.21) min in 46 patients. Deep hypothermic circulatory arrest (DHCA) was performed in 43 patients, and the duration was 2-109 (30.00?22.37)min. The lowest nasopharyngeal temperature was 13.6-25.7 (19.61?3.40)℃, and the lowest rectal temperature was 15.2-30.5 (21.58?3.63)℃. Ultrafiltration technique was used in 49 cases. 36 of 54 cases (66.7%) self-awakened. Four cases (7.41%) died in the early post-operation period. Conclusion The DHECC can offer a clear and exsanguinated operating field, and is helpful to execute complicated or major operations which are hard to complete with common techniques.