Objective To observe the effect of puerarin injection (PI) on insulin resistance in gestational diabetic mellitus(GDM) rats. Methods Rats with gestation for 5 days received onedose injection of STZ 35 mg/kg into subaortic caudal vein. After injection for 72 hours, rats with blood sugar over 13.5 mmol/L indicated the modeling being successful. Then the model rats were divided into treatment group (TG, using Puerarin), westernmedicine control group(WCG, using rosiglitazone) and model group (MG, using 0.9 % NaCl), 10 rats in each group .Ten normal gestational rats were used as normal control group (NCG, using 0.9 % NaCl).Two weeks later, the changes of blood sugar, Creactive protein (CRP) and tumor necrosis factor- ? (TNF- ? ) were observed. Results Compared with MG, the above indexes were improved in TG and WCG after treatment( P

Objective] To summarize teacher Qin’s clinical experience in treating premature ovarian failure by mainly reinforcing kidney. [Method] Collect the clinical cases, and analyze the etiology and pathogenesis, syndrome differentiation and treatment of premature ovarian failure for teacher Qin ’s. [Result] Teacher Qin thought that this disease was mainly due to kidney deficiency, especially kidney-yin deficiency, and accompanied by liver depression. This disease can be divided into kidney-yin deficiency, kidney-yang deficiency, deficiency of kidney-yin and yang, deficiency of kidney and liver depression. Reinforcing kidney-yin for kidney-yin deficiency; reinforcing kidney-yang for kidney-yang deficiency; reinforcing kidney-yin and yang for deficiency of kidney-yin and yang; tonifying the kidney essence and soothing liver-qi stagnation for deficiency of kidney and liver depression. [Conclusion] Treatment of premature ovarian failure is based on tonifying kidney, supplement on soothing liver, while some change therapies, should combine disease with syndrome.

With the improvement of China's population aging and health care consciousness, female pelvic floor dysfunction gets increasing attention in society. Modern TCM scholars conclude TCM doctors’ theories about etiology and pathogenesis of pelvic floor dysfunction, summarized as debility of Chong Ren meridians and inability to lift, and then they advocated invigorating qi and elevating yang, with a purpose to protect pelvic floor stability. This article conducted relevant discussion on the significance of rehabilitation of pelvic floor dysfunction based on TCM adjusting qi activity.

Objective To observe the effect of Chinese medicine of Gukang(GK) on bone mineral density(BMD) in osteoporosis rats.Methods Seventy-two female SD rats were randomized into 6 groups: pseudo-operation group,model group,nilestriol group(in the dose of 1mg/kg),and high-,middle-and low-dose GK groups(9.6,4.8 and 2.4 g?kg-1?d-1 respectively).Rat models of osteoporosis were induced by removal of bilateral ovaries.Three months after operation,the rats were given the corresponding medicine according the experimental design.After treatment for 3 months,in-vivo BMD as well as the in-vitro BMD in the isolated left femur and tibia was detected with dual energy X-ray bone densitometer.Results The in-vivo general BMD and lumbar BMD of the model group were decreased(P

AIM:To treat the chemotherapy-induced premature ovarian failure ( POF) rats with the methods of tonifying kidney yin and yang by observing the indicator changes in apoptosis of ovarian granulosa cells and related cyto -kines before and after medication .METHODS:SD rats ( n=36) were chronically given intraperitoneal injection of cyclo-phosphamide ( CTX) for 15 d in order to establish the animal model of POF .The rats were randomly divided into control group, CTX group, CTX+kidney yin group and CTX +kidney yang group.The levels of tumor necrosis factor alpha (TNF-α) and interferon gamma ( IFN-γ) in the peripheral blood were detected by ELISA .The apoptosis of ovarian granulosa cells was observed by TUNEL method .The levels of apoptosis related proteins were determined by Western blot .RE-SULTS:After modeling , the levels of TNF-αand IFN-γin the peripheral blood were significantly increased .The protein expression of Bax was up-regulated and Bcl-2 was down-regulated .The apoptosis of ovarian granulosa cells was significantly induced with a deep staining .After treatment with tonifying kidney yin and yang , the levels of TNF-αand IFN-γin the pe-ripheral blood were decreased , the protein expression of Bax was down-regulated and Bcl-2 was up-regulated, and the apop-tosis of ovarian granulosa cells was reduced .CONCLUSION:The reserve function of the ovarian can be recovered by pre-scriptions of tonifying the kidney yin and yang via regulating the expression of TNF -α, IFN-γ, Bax and Bcl-2, and inhibi-ting the apoptosis of ovarian granulosa cells .

Objective To explore the atypical ultrasonic appearances of breast fibroadenoma and analyze the cause of mis diagnosis.Methods A total of 493 lesions in 485 patients of breast fibroadenoma detected by ultrasound examination andconfirmed by pathology were retrospectively analyzed.Atypical appearances were analyzed and misdiagnosis rate were cal-culated.Statistical methods were taken to compare the misdiagnosis rates in lesions with various atypical appearances andthose with none or single atypical appearance.Misdiagnosis rates in lesions with different sizes and pathological types wereanalyzed statically.Then misdiagnosing causes were analyzed.Results A total of 404 lesions were diagnosed correctly,in-cluding 99 lesions with atypical appearances,and the other 89 lesions were misdiagnosed,which showed atypical appear-ances.The misdiagnosis rate of lesions with ≥2 atypical features was higher than that with none or single atypical feature(x2 =256.40,P＜ 0.05).Large lesions (maximum diameter＞ 3 am) showed higher misdiagnosis rates than small ones(maximum diameter ≤3 cm,x2=6.73,P＜0.05),and complex fibroadenoma lesions also showed higher misdiagnosisrate than simple ones (x2 =188.01,P＜0.05).Conclusion The lesions with various atypical appearances,large size andcomplex fibroadenoma in pathology are easy to be misdiagnosed.

BACKGROUND: 5-azacytidine (5-Aza) has been frequently used to induce bone marrow mesenchymal stem cells (BMSCs)differentiation into cardiomyocyte.OBJECTIVE: To observe expression of cardiomyocyte-related receptors in cardiomyogenic differentiation of rat BMSCs.METHODS: BMSCs of passage three were assigned to four groups: group Ⅰ: L-DMEM solution alone was replaced; Ⅱ:L-DMEM solution was replaced after induction of 100 mg/L AST+5 μmol/L 5-Aza for 24 hours; group Ⅲ: L-DMEM solution wasreplaced after induction of 10 μmol/L 5-Aza for 24 hours; and group Ⅳ: L-DMEM solution was replaced after induction of 5 μmol/L5-Aza for 24 hours. Culture medium was replaced every 3 days in each group. Differentiated cells were identified after 30 days ofinduction.RESULTS AND CONCLUSION: Expression of cardiomyocyte specific proteins Nkx2.5, cTnT and Desmin was detected in groupsⅢ, Ⅳ and Ⅱ after induction compared with group Ⅰ , with significant differences (P < 0.01). The amount of cTnT and Desminexpression expression was significantly higher in groups Ⅱ and Ⅲ compared with group Ⅳ (P < 0.01). The level of Nkx2.5expression was significantly higher in groups Ⅱ (P < 0.01) and Ⅲ (P < 0.05) compared with group Ⅳ. No Nkx2.5, cTnT andDesmin espression was detected in group Ⅰ. After induction for 2 weeks, cells with spontaneous contractility were observed ingroups Ⅱ and Ⅲ, indicating differentiation towards cardiomyocyte after induction. Results demonstrated that induction effectswere similar between 100 mg/L AST+5 μmol/L 5-Aza and 10 μmol/L 5-Aza. This may contribute to cytoprotective effects of AST,which can promote vascular endothelial cell proliferation, enhance celss tolerance to 5-Aza-induced cytotoxicity and upregulatecardiac-specific protein expression.

Experimental pathology is an important part of life science research associated with animal experiment. Acquisition and fixation of optimum specimen and subsequent section of paraffin embedded tissue and dyeing are key factors playing important role in reliability, authenticity of pathological diagnosis.This paper summarizes the problems encountered in pathological section making of animal experiment and it correspond solutions.

This paper introduces the structure and the equipment of special artificial environmental and experimental chamber and its basic operating requirements. In compliance with the national standard and safety management, the safety, effectiveness and controllability of the chamber are described.

OBJECTIVE:To provide reference for understanding the significance of drug proportion control deeply and future direction of hospital reform. METHODS:The data were collected from the relevant statistical reports of our hospital in August 2016,June and August 2017. The data were analyzed statistically and compared in respects of drug proportion,income,medical cost,ratio of essential medicine types,prescription amount(outpatient and emergency department),utilization rate and utilization density(AUD)of antibiotics in whole hospital,outpatient and emergency department,inpatient department. RESULTS:The drug proportion of our hospital was decreased significantly in Aug. 2017(after drug price reform),which fell by 13.35% from the previous month and 17.58% year-on-year. Hospital revenues were on the rise,which increased by 6.92% from the previous month and 11.45% year-on-year. Outpatient and emergency income was decreased slightly from the previous month(by 2.73%). Inpatient income was increased significantly from the previous month(by 9.76%). Per capita medical cost in outpatient and emergency department were increased,which increased by 4.01% from the previous month and 9.62% year-on-year;per capita medical cost of inpatients were declined,which fell by 0.91% from the previous month and 3.11% year-on-year. The ratio of essential medicine types in the whole hospital was decreased slightly from the previous month but increased by 17.79% year-on-year. Drug amount of outpatient and emergency prescriptions was decreased by 11.34% from the previous month and prescription amount decreased by 9.16% year-on-year. The utilization rate of antibiotics in the whole hospital was decreased by 8.10% from the previous month;AUD was decreased by 3.88% from the previous month and 13.88%year-on-year. CONCLUSIONS:After drug price reform,drug proportion of our hospital is effectively controlled,which has a positive influence on the operation of medical service system and patient medical cost. At the same time,some problems that need to be solved are also exposed,such as increase in per capita medical costs and the decrease in the number of outpatient and emergency patients. So,drug proportion control is only the first step in deepening hospital reform,and the follow-up motivation still needs to be provided continuously through strengthening rational drug use.