Objectives To develop and evaluate a real-time fluorescent quantitative RT-PCR method for the detection of a novel CXC chemokine macrophage inflammatory protein-2?(MIP-2?)mRNA expression based on TaqMan technique.Method The expression of MIP-2? mRNA was detected by TaqMan real time fluorscet quantitative RT-PCR. Results The dynamic range of the assay varied from 102 to 107 copies. The intra- and inter-assay coefficient variation (CV) were less than

Objective To investigate the level of self-care behaviors and self-efficacy among adult type 2 diabetes patients in community and discuss current problems.Methods A convenient sample of 85 patients with type 2 diabetes in Haiyuncang community was interviewed,using diabetes self-care scale and diabetes self-efficacy scale.Totally 85 questionnaires were got and underwent analysis.Results The index score of self-care behaviors and self-efficacy among adult type 2 diabetes patients in community were 66.98% and 69.02%.There were no significant difference in self-care behaviors of patients whether they attended diabetes lectures or not.Self-efficacy correlated significantly with self-care behaviors.Conclusions The self-care behaviors and self-efficacy of diabetes patients need to be improved,and diabetes education in community should be emphasized and carried out effectively.

[Objective]To study the effect of End-to-side neurorrhaphy for protection target muscle.[Method]Twenty-six wistar rats were divided into two groups,with 13 in each group,peroneal nerve was transacted on the right side of all the animals,in one group,the proximal end of peroneal nerve was turned back and ligated,the distal end was sutured to the side of tibial nerve with perineurial window,in another group,both ends of the transacted peroneal nerve were turned back and ligated as contrast,no operation was done on the left side.Three months after operation,the peroneal nerve and the tibial muscle were taken for histologic exmination,and the muscle's wet weight was examed.[Result]Significant difference was seen between the two groups in the muscle wet weight,the muscle fiber cross sectional area,the moter endplate area and color(P

Through combing the objects of the medical digital resources of long-term preservation,analyzing the characteristics of medical digital resources,and discussing challenges coming from medical big data,the paper researches the long-term preservation architecture of medical digital resources primarily from life cycle,standard specification,storage architecture,monitoring management and preservation mode to provide effective guidance for comprehensive long-term preservation construction of medical digital resources.

In order to further probe into the therapeutic effect and mechanisms of Shou Wu Huan Jing Capsule for treatment of male infertility, 180 patients were investigated. Results showed that the prescription had reliable therapeutic effect with no side - effect, and the effective rate was 93. 89%. Effects of Shou Wu Huan Jing Capsule. on human sperm moment velosity, index of vitality, capillary penetrativity test and sperm hypotonic expansion test were analysed for the application in improving the seminal quality of male infertility patients in culture of human sperm with extract of Shou Wu Huan Jing Capsule. The results revealed that it could promote motor ability and membrane function of sperm. Thus, it is of potential application value for treatment of male infertility and in auxiliary fertility technique as extrinsic additive.

Objective This research is to assess the heterozygosity of Tbx1 gene on 22q11 2 in the patients with conotruncal heart malformation.Methods By fluorescence in situ hybridization (FISH) with partial segment of Tbx1 gene, we examined 22 patients with conotruncal heart malformation including 5 syndromic cases and 17 isolated cases. Northern blot was performed with RNA of 50 human tissues.Results Two of 5 syndromic patients had chromosome 22q11 2 hemizygote microdeletion of the Tbx1 gene, while 17 isolated patients did not show such deletion. Northern blot showed that there were Tbx1 gene positive expression in skeletal muscle, testis, lung and fetal heart.Conclusions Our study suggests that Tbx1 gene may be one of the pathogenic gene related to CATCH22.Association of the Tbx1 gene and conotruncal heart teratogenic gene is to be further detected in gene mutation of patients without heterozygosity deletion.

Objective To study the results of treatment of open fracture of tibia and fibula by external fixation with unilateral multifunction external fixation apparatus(UMEFA) and plaster in different period. Methods 89 cases with open fracture of tibia and fibula had got reduction and external fixation with UMEFA and then divided into two groups, group A of 53 cases followed by external fixation with UMEFA, 8 cases of them were forced to remove UMEFA because of complications, the other 45 cases followed by external fixation with UMEFA till bone union, group B of 36 cases were removed UMEFA and changed with plaster when the skin was healed, the sweal was eliminated and the bone was steady. Compared and analyzed the results by complication, healing time and quality.Results In group B, there was less complications(P

Objective To study the role of chronic prostatitis(CP)in the pathogenesis and development of IgA nephropathy(IgAN). Methods The incidence rate of CP was compared between the patients with IgAN and the patients with non-IgA mesangial proliferative glomerulonephritis. Furthermore, the clinical and pathological features were compared between the IgAN patients without CP and those with CP. The therapeutic effect of anti-inflammatory treatment in IgAN patients with CP was evaluated. Results Incidence of CP in IgAN patients was 44.7% and that in non-IgA mesangial proliferative glomerulonephritis patients was 21.6%. There was significant difference in the incidence of chronic prostatitis between the two groups. The incidence of glomerular sclerosis and the deposition of immune complexes in vascular wall in IgAN patients with CP were higher than that in patients without CP. The treatment of CP may be beneficial in ameliorating IgAN in some patients. Conclusion Chronic prostatitis is one of the probable pathogenetic factors in IgAN.

Objective To explore the medication rules of Professor Gao Zhongying in treatment of chronic atrophic gastritis on basis of his curative prescriptions.Method Prof.Gao Zhongying clinic medical records were used as data resource.Database was built by using Traditional Chinese Medicine Inheritance Support System (Version 2.5).The core combination and assocical rules among the drugs were confirmed by frequency analysis and assocical rules analysis methods.Results We collected 60 clinical formulas including 83 kinds of Chinese herbs.The bighest frequency used simply drugs were Fritilariae Thunbergh Bulbus,Trichosanthis radix,Galli Gigerii Endothelium Corneum and so on.The most frequency drug combination were Fritilariae Thunbergh Bulbus,Trichosanthis radix,Fritilariae Thunbergh Bulbus,Galli Gigerii Endothelium Corneum,Trichosanthis radix,Galli Gigerii Endothelium Corneum,Fritilariae Thunbergh Bulbus,Curcumae Rhizoma and so on.The drug association rules of drug combination were 447 items.Conclusions The medication rules embodied the thoughts of Professor Gao Zhongying of replenishing qi to invigorate the spleen and regulating the stomach to moisten dryness was the main method in treatment of chronic atrophic gastritis and deficiency of spleen and dryness of stomach,which was the key pathogenesis in occurrence and development of chronic atrophic gastritis.

OBJECTIVE To explore the suitable hospital infection control measures in health centers of poverty-striken villages,in order to improve the management of hospital infection,decrease hospital infection rate and protect the health of medical staff and patients.METHODS The status quo of hospital infection in health centers of poverty-striken villages,was investigated in 20 small towns health centers with were randomly divided into two groups:test group(n=15)and control group(n=5).The suitable hospital infection control measures were explored from 5 points.The effect of infection control by before-after controlled study of experimental group and randomized controlled study of control group was anal yzed.RESULTS The rate of hospital infection in test group was decreased from 7.60% to 1.98% and at in control group didn't change,the difference was significant.CONCLUSIONS The managements of establishment of the suitable hospital infection control measures in health centers of poverty-striken villages have been put into practice and gained good result.