Objective: To survey and analyze risk factors for coronary artery stenosis in young and middle-aged people to provide thinking for prevention and treatment of atherosclerosis. Methods: The risk factors of 167 patients with coronary artery stenosis proved by coronary angiography from Mar 2011 to Jun 2012 were retrospectively analyzed. Results: Logistic multiple regression analysis indicated that risk factors of coronary artery stenosis in turn were hypertension（1.784），hyperlipidemia（1.708），smoking（1.095）, waist-hip ratio（0.877）, family history of diabetes mellitus（0.781）, high homocysteine（0.608）, serum matrix metalloproteinase-9（0.579）,obesity（0.458）, lack of exercise（0.427）, P<0.01 all according to β value,. Conclusion: Early prevention focusing on relative risk factors may play a vital role for prevention and treatment of atherosclerosis in young and middle-aged people.