1.Determination of Fructose and Glucose in Multiple Electrolytes and Invert Sugar Injections by HPLC with Refractive Index Detector
Wei DONG ; Jiagang CHEN ; Bin LU
China Pharmacist 2014;(11):1883-1885
Objective:To establish an HPLC method with a refractive index detector for the simultaneous determination of fructose and glucose in multiple electrolytes and invert sugar injections. Methods: A Waters XbridgeTM Amide(150 mm × 4. 6 mm,3. 5 μm) column was used with acetonitrile-water -triethylamine (75∶25∶0. 2) as the mobile phase, the column temperature was 40 ℃, the flow rate was 1. 0 ml·min-1 , the refractive index detector was used with the detector temperature of 40 ℃, and the injection volume was 50 μl. Results:A good linear relationship between the quantity of glucose or fructose and peak area was shown within the range of 99. 8-149. 7 μg(r=0. 999 7)for fructose and 100. 3-150. 4 μg(r=0. 999 2)for glucose, the average recovery of fructose and glucose was 99. 2% and 99. 5% with RSD of 0. 81% and 0. 84%(n=9), respectively. Conclusion: The method is fast and convenient with high specificity, good accuracy and promising separation, which can be used in the content determination of fructose and glucose in multiple electrolytes and invert sugar injections.
2.The experience of diagnosis and treatment for pancreatic cystic neoplasms
Xuebin CHENG ; Jianping GONG ; Jiagang DONG
Journal of Endocrine Surgery 2009;3(2):97-99
Objective To explore the diagnosis and treatment for pancreatic cystic neoplasms.Methods The clinical data of 10 cases with pancrestic cystic neoplasms treated from 2004 to 2008 was reviewed and both domestic and internal pertinent literatures were summarized.Results The pancreatic cystic neoplasms mostly happened in middle-aged women.The clinical manifestations showed no specificity.Ultrasonography, CT and MRI could offer important sign for diagnosis.The rate of surgical resection was high and the prognosis was satis-factory.Conclusions The pancreatic cystic neoplasms is being muh with the development of imagings.It is dif-ficuh to distinguish the feature of the cystic neoplasms before surgery, so surgerical resection should be done for all cystic tumors found.
3.Experimental Study on CompoundWeifu in Gout Treatment
Lizhen HUANG ; Lingling YANG ; Jiagang DENG ; Zhengcai DU ; Dong LU
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(7):1409-1413
This article was aimed to study the action of antigout effects of compoundWeifu. Through the acute gouty arthritis models of rabbits and rats induced by micro-crystal sodium urate (MSU). The histopathological changes of synovial tissues among rabbits were observed. And the contents of TNF-α, IL-1β, IL-6 in rabbit serum were determined. The swelling degree of the rats’ feet and the content of prostaglandin E2 (PGE2) from the inflammatory exudate foot induced by MSU were also determined. The antigout effects of compoundWeifu were observed. The results showed that the grade of the vascular congestion, swelling and inflammatory cell infiltration in synovial tissues in the high-dose and medium-dose CompoundWeifu group had significantly improved (P <0.05). And the levels of TNF-α, IL-1β, IL-6 in serum were significantly decreased (P< 0.05, orP < 0.01). It can obviously reduce the swelling degree induced by MSU, and the level of PGE2 in the inflammatory exudate foot (P< 0.05, orP < 0.01). It was concluded that CompoundWeifu had obvious antigout effects in the study.
4.The effects of a novel standardized training program on satisfaction and training quality of general practitioners
Lang LI ; Liang DONG ; Huachun ZHU ; Jiagang CHEN ; Chundong WU ; Yong YAO
Chinese Journal of Medical Education Research 2016;15(6):561-564
Objective To investigate the effects of a novel standardized training program on the satisfaction and training quality of general practitioners (GPs). Methods 114 GPs trained in Wuxi People's Hospital between 2009 and 2013 were included, 52 GPs and 62 GPs were trained before and after the reform respectively. The measures of teaching reform included improvements in curriculum provision, theory teaching, learning modality and skills training. The evaluation indexes of satisfaction questionnaire and examination were confirmed by literature method and small-group discussion. Self-report satisfaction ques-tionnaire were distributed to graduate GPs and valid questionnaires were collected, while the results of entrance examination and graduation examination of GPs were recorded before and after the teaching reform. The research data were analyzed by chi-square test using SPSS 16.0 software. Results The basic characteristics of GPs and results of entrance examination before and after the teaching reform were compa-rable (P>0.05), however, GPs' satisfaction with curriculum, theoretical education, study form and skill training were increased significantly after teaching reform (P<0.05). Besides that,the results of graduation examination of GPs after the teaching reform, both the theory and skill examination, were improved signifi-cantly (P<0.05). Conclusion The study showed that GPs satisfaction with training modality and the results of graduation examination were improved greatly through our innovative teaching reform.
5.Optimize operation process and operation technique to reduce intraoperative and postoperative complications of laparoscopic splenectomy
Jiagang DONG ; Zuojin LIU ; Ke YOU ; Jianping GONG
International Journal of Surgery 2018;45(9):616-621
Objective Laparoscopic splenectomy has become an effective method to treat the hypersplenism and splenomegaly caused by various diseases.Although laparoscopic technology is very mature at present,laparoscopic splenectomy is still associated with many complications.This study was based on the comparison of different processes and operation techniques of laparoscopic splenectomy,to summarize a more effective operation process and operation technique which can reduce the related complications of laparoscopic splenectomy.Methods The study reviewed the clinical data of 130 patients'who underwent laparoscopic splenectomy in our department from September 2015 to December 2017.According to different operation mode and operation technique these patients were divided into 6 groups.Operation process groups:group Ⅰ for arterial priority group (open the gastrocolic ligament and expose splenic artery,then dissociate ligament around the spleen,and finally divide from the spleen pedicel) had 73 cases;group 2 with the way of building a tunnel beneath spleen pedicel and dissecting spleen in situ had 57 cases.With regard to the way of breaking spleen pedicel:group 3 using stapler to break spleen's pedicel included 101 cases;group 4 using dissecting the secondary spleen pedicel included 29 cases.Whether blocking the splenic vein:group 5 whose splenic vein was not ligated had 95 cases;group 6 whose splenic vein was ligated coverd 35 cases.Analysis and comparison between groups about intraoperative and postoperative complications related to different groups were made.Measurement date were expressed as (-x ± s),t test was used for comparison between groups.Count data was expressed as rate(%),and chi-square test was used for comparison between groups.Results Group 2 has less blood loss [group 1:(356.0 ± 96.2) ml versus group 2:(220.9 ± 57.3) ml,P =0.016],shorter operation time [group 1:(156.0 ± 36) min versus group 2:(120.9 ± 55.4) min;P =0.038] and fewer complications [group 1:9.6% (7/73) versus group 2:5.3% (3/57);P =0.035].When compared with group 4,group 3 have more advantages in the intraoperative blood loss [group 3:(256.0 ± 85.1) ml versus group 4:(380.9 ± 107.3) ml;P=0.019],the operation time [group 3:(131.0±30.9) min versus group 4:(171.9 ±61.2) min;P=0.024],and the postoperative complications [group 3:6.95 % (7/101) versus group 4:10.3 % (3/29);P =0.048].When compared with group 5,group 6 has more advantages in the intraoperative blood loss [group 5:(250.0 ± 56.2) ml versus group 6:(320.9 ± 87.3) ml;P =0.012],the operation time [group 5:(136.0 ± 36.2) min versus group 6:(180.9 ± 57.3) min;P =0.013],and the postoperative complications [group 5:6.3% (6/95) versus group 6:11.4% (4/35);P=0.013].Conclusion Both the procedure of tunnel procedure in situ cutting spleen and the application of stapler can reduce the amount of bleeding,shorten the operation time,and reduce the occurrence of postoperative complications.The ligation of the splenic vein is not necessary because it increases the operation time,the risk of bleeding,and the occurrence of postoperative complications.
6.Assessment report on infection control of schistosomiasis in China, 2008
Yang HAO ; Donghua YI ; Xianfeng ZHANG ; Jijie XIONG ; Wenzong YUAN ; Shoujing HU ; Xiaohua WU ; Rong ZHU ; Jiagang GUO ; Xibao HUANG ; Yuesheng LI ; Honggen CHEN ; Tianping WANG ; Xingqi DONG ; Huazhong LI ; Canjun ZHENG ; Zhao CHEN ; Liying WANG ; Xiaonong ZHOU
Chinese Journal of Schistosomiasis Control 2009;21(6):457-463
According to the requirement of the national assessment for achieving the infection control criteria, 42 villages (among them,25 villages belonged to the first stratum, and 17 villages belonged to the second stratum) in 14 counties from 5 provinces, including Hunnan, Hubei, Jiangxi, Anhui and Yunnan, were selected as sampling villages for the assessment.The results from the field assessment showed that 154 out of 9 067 people were found infected with Sckistosoma japonicum, with an average infection rate of 1.7% ranged from 0.31 % to 4.10% , and only Yongping Village from Weishan County and Tenglong Village from Eryuan County were not found any case. A total of 46 out of 3 323 head of cattle were infected with S. japonicum, with an average infection rate of 1.38% ranged from 0.26% to 3.79% , and no any infected individual detected in Nanling County. No outbreak occurred in those sampling villages. Therefore, it is indicated that the five sampling provinces have reached the national criteria on infection control of schistosomiasis.
7.Rapid identification of Oncomelania hupensis snail habitat in the Poyang Lake region by Geographic Information System (GIS) and Remote Sensing (RS).
Jiagang GUO ; Dandan LIN ; Guanghan HU ; An NING ; Hongyun LIU ; Shangbiao LU ; Dong LI ; Xiaohua WU ; Rongrong WANG ; Minggang CHEN ; Jiang ZHENG ; Marcel TANNER
Chinese Journal of Epidemiology 2002;23(2):99-101
OBJECTIVETo identify Oncomelania hupensis snail habitats and areas with high transmission potential by GIS/RS.
METHODSMarshland areas near high endemic villages of schistosomiasis in the Poyang Lake region were selected. Corresponding map was digitized and (Landsat 5 TM) image was corrected according to the digital map. The image in dry seasons was calculated by both normalized difference vegetation index (NDVI) and tasseled cap model.
RESULTSResult showed that snails spots were distributed in class 6, 7 and 8. Farther analysis of both NDVI and tasseled cap model showed that the snail habitats were mainly distributed in the areas where NDVI value was more than 110, and in tasseled cap wetness value between -10 to 3 with correction rate 94.93%.
CONCLUSIONFirst step was to use unsupervised classification to define the class 6, 7 and 8 snail habitat environment. Second step was to extract the value by NDVI model, and to define a healthy vegetation as snail suspicious habitat when NDVI value was more than 110. Then the third step was to use tasseled cap wetness model to define the areas as snail habitats which value was between -10 to 3.
Animals ; Demography ; Disease Vectors ; Schistosomiasis ; transmission ; Snails
8.Investigation on compliance of forbidden depasturing livestock on marshland with Oncomelania snails in schistosomiasis endemic areas
Chunli CAO ; Jing WANG ; Ziping BAO ; Hongqing ZHU ; Shunxiang CAI ; Yiyi LI ; Dong LI ; Jiachang HE ; Leping SUN ; Xianhong MENG ; Bo ZHONG ; Xiguang FENG ; Zhengming SU ; Jun LI ; Xiaonan GU ; Hao WANG ; Weiwei RU ; Weisheng JIANG ; Shizhu LI ; Qiang WANG ; Xiaonong ZHOU ; Jiagang GUO ; Gengming ZHAO
Chinese Journal of Schistosomiasis Control 2009;21(6):482-485
Objective To study the compliance of forbidden depasturing livestock on the marshland with Oncomelania snails in schistosomiasis endemic areas. Methods According to 3 levels of human infection rates as > 10% ,5%-10% and <5% , 2 204 residents selected randomly from the schistosomiasis endemic villages were sampled with the stratified cluster sampling method in Hunan, Hubei, Jiangxi, Anhui, Jiangsu, Sichuan and Yunnan provinces, and investigated by questionnaire. The contents of the questionnaire included the recognition and implementation of forbidden depasturing livestock on marshland with Oncomelania snails and breeding livestock in bam. Results A total of 78.4% residents agreed forbidden depasturing livestock on marshland with snails, but 3. 7% residents disagreed it. A total of 83. 9% residents considered the relationship between breeding livestock in bam and schistosomiasis control, but 3. 1% residents thought that it was no relationship. The main reasons of depasturing livestock on marshland with Oncomelania snails were the high cost of breeding livestock in bam (36. 2% ) , unaccustomed (26.4% ) and no room for breeding livestock in bam (25.4% ). Conclusion Forbidden depasturing livestock on the marshland with Oncomelania snails should be strengthened according to the local economic, nature environment, agriculture, residents'culture degree and agriculture habit.