Objective To evaluate the effects of intravenous anesthesia with propofol and fentanyl on flexible fiberoptic bronchoscopy (FFB).Methods Forty ASA Ⅰ - Ⅱ patients undergoing elective FFB were randomly divided into two groups (20 cases in each group): control group and intravenous anesthesia group.All patients received airway local topical anesthesia with 2% lidocaine before the procedure.The patients in intravenous anesthesia group received propofol 1.5 mg/kg and fentanyl 1 μ g/kg intravenous injection for induction while in control group normal saline was given instead.Supplemental oxygen was administered by the endoscopy face mask.The mean arterial pressure (MAP),heart rate (HR)and pulse oxygen saturation (SpO_2) were continuously monitored and recorded before induction of anesthesia (T_1, baseline), 1 min after intravenous injection (T_2) ,immediately and 3 min after intubation (T_3,T_4),after FFB (T_5).The persistence time of the procedure was noted.When the patients responded to commands exactly, the recovery time was recorded.All patients were interviewed for the global tolerance to the procedure, the acceptance of another fiberoptic bronchoscopy and the degree of amnesia.Results All of the 40 patients finished FFB successfully.VAS postoperation and dependence scores were lower in intravenous anesthesia group than those in contrel group[(1±2),(2±3) scores and (7±1),(7±3) scores](P< 0.05).MAP and HR at T_3, T_4 and T_5 were significantly increased compared with baseline values at T_1 in control group (P <0.05).MAP and HR at T_2 was lower than those at T_1,HR at T_3 was higher than that at T_1 in intravenous anesthesia group (P < 0.05 ).Compared with control group, MAP and HR at T_3, T_4 and Ts were lower, HB at T_2 was lower in intravenous anesthesia group (P < 0.05).There was no awareness during the FFB in intravenous anesthesia group.Conclusion Intravenous anesthesia with propofol and fentanyl is safe and effective for FFB.

Objective To study the relationship between the inherit gene and the pharmic efficacy by observing the apolipoprotein E polymorphism and the nylestroil effect in lowering serum lipids in postmenopausal women. Methods Ninety-four postmenopausal women were divided into three groups according to their types of the apoE gene: E2, E3 and E4. Thirty-six women who received nylestroil therapy were compared with controls, 58 women who had not received the therapy, in lipids, apolipoprotein E and lipoprotein(a)〔LP(a)〕. Results Compared with the control groups, the treatment groups showed an increase in HDL-C (high-density lipoprotein) and decrease in LDL-C(low-density lipoprotein) and LP(a). Though Group E2 showed an increase in the HDL-C and TG and a decrease in the LDC-C and LP(a), no significance was shown compared with the controls. Group E3 was increased in the HDL-C(2.7?0.9)mmol/L and decreased in the LDL-C (3.2?1.0)mmol/L and Lp(a) (0.4?0.2)g/L, and the differences were significant in HDL-C(1.7?0.5)mmol/L and Lp(a) (0.5?0.3)g/L compared with the controls. Group E4 was increased in the HDL-C (2.6?1.0)mmol/L and decreased in the LDL-C(2.5?1.6)mmol/L, TC and LP (a), and the differences were statistically significant(P

OBJECTIVE To analyze the resistance of Pseudomonas aeruginosa (PAE) isolated from clinical specimen and provide the guidance for the clinical treatment. METHODS The P. aeruginosas infection status from Jun 2005 to Dec 2007 was reviewed retrospectively,and the results of susceptibility test in 286 strains of PAE were analyzed. RESULTS The drug-resistance rates to gentamicin,cefotaxime,and ceftriaxone in PAE were all above 60.0%,and that to cefoperazone/sulbactam,piperacillin/tazobactam sodium,amikacin and levofloxacin showing all higher sensitivity. The resistance rates to meropenem and imipenem were 17.1% and 18.5%,respectively. CONCLUSIONS P. aeruginosa is one of the main pathogenic bacteria in nosocomial infection. It's very important to strengthen the monitoring of drug-resistance of PAE and rationally antibiotics usage.

OBJECTIVE To investigate the resistance of meticillin-resistant Staphylococcus aureus(MRSA) and the occurrence of gene erm.METHODS ATB Staph and microdilute tests were performed to detect the susceptibility to 15 kinds of antibiotics in 50 strains of the S.aureus(SAU).Gene erm of these strains was detected by polymerase chain reaction(PCR).RESULTS There were no strains resistant to vancomycin,teicoplanin,fusidic acid and quinupristin-dalfopristin in 42 strains of MRSA detected.There were no strains sensitive to penicillin,oxacillin,gentamicin,tetracycline,ciprofloxacin and levofloxacin.Thirty-five strains habored gene erm in 42 strains of MRSA.The positive rate of gene erm was 83.3%.CONCLUSIONS The multiple-resistance of the MRSA is a serious issue.The resistance to erythromycin in MRSA is mediated by gene erm which encodes the methylase and changes the target site of drug action.

Objective To investigate the clinicopathological features,diagnosis and treatment experience,in order to improve the recognition of male breast cancer and prepare for the study of standardized treatment of breast cancer.Methods Data of epidemiological characteristics,clinical and pathological parameters,treatment and outcome from 43 male breast cancer patients were collected and analyzed in Peking University Cancer Hospital and Beijing Hospital from January 2009 to December 2014.Results Of these patients,42 (97.6％) cases presented with located tumors,11 (25.5％) cases were on pathological stage Ⅲ or stage Ⅳ,and 40 (93.0％) cases were with hormone receptor positive breast cancer and 3 (6.9％) cases were with human epidermal growth factor receptors (HER)-2 positive breast cancer.Patients were stratified according to more or less than 70 years of age,and there were no significant differences in clinicopathological features between the two year groups.But 3 cases with HER-2 over-expressed were all less than 70 years old.38 (88.4％) patients underwent surgical treatment,among which 32 HR-positive patients (74.4％) received tamoxifen as adjuvant endocrine therapy.The median follow-up periods was 31 months (6.1-55.4 months),7 (16.3％) patients developed local recurrence or distant metastasis.Conclusions Male breast cancer is often diagnosed at a later stage and has inferior outcome.Majority of male breast cancer are found to be HR positive,hence hormonal therapy should be strongly considered.

OBJECTIVE: To establish RP-HPLC method for the fast determination of floxuridinein in serum and homogenate of some of the organs of mice. METHODS: The determination was performed on C18 chromatographic column with its mobile phase consisted of phosphate buffer(pH= 7.4) - methanol(95∶5) and with its detection wavelength at 268nm. RESULTS: Linear detection concentration ranges of floxuridinein were all at 15～240?g/ml. The minimum detection concentration was 3?g/mL(S/N≥3). The average extraction recovery was above 95%. The average recovery ranged from 91%to 112%. Both the intra-day and inter-day precisions were less than 10%. CONCLUSION: The method is simple, rapid, reliable and economical, and it can be used for the determination of serum floxuridinein concentration and the study of its body distribution in animals.

OBJECTIVE:To optimize the preparation techniques for floxuridinyl diacetate solid lipid nanoparticles(FUDRA-SLN).METHODS:The FUDRA-SLN was prepared by film-ultrasound dispersion method with soybean phos?pholipids as carrier,and the preparation techniques were optimized by means of an orthogonal test design with entrapment ef?ficiency and appearance as indexes of investigation.RESULTS:The FUDRA-SLN prepared in the optimized techniques was even and regular in appearances,with particle diameter at(215?83)nm,entrapment efficiency at98.27%and drug loading dosage at8.20%.CONCLUSIONS:FUDRA-SLN prepared by the optimized film-ultrasound dispersion method has a high entrapment efficiency and high drug loading dosage,and this method is suitable for the laboratory preparation.

OBJECTIVE: To develop RP-HPLC method for the rapid determination of resveratrol in serum and tissue homogenates (in some organs) of mice.METHODS:HPLC determination was performed using Kromasil C18 column,the mobile phase consisted of methanol - water (48∶ 52) with a flow rate of 1ml/min,the column was under room temperature with its pressure at 12kPa,the detection wavelength was 303nm and the sample size was 20?l. RESULTS: The linear range for resveratrol was 0.25～25?g/ml, with minimal detectable drug concentration at 0.1?g/ml (S/N=3). The average absolute rec_overy of the samples was above 88% and the methodological recovery rates of which ranged from 96% to 100%,RSD were below 10%. CONCLUSIONS: The established determination method is economical, reliable, simple and rapid, and suitable for the blood concentration determination of resveratrol and the study of its distribution in animal body.

OBJECTIVETo determine 5 phenolic acids in the soils around rhizosphere of Rehmannia glutinosa in the field under normal rotation and successive cropping.

METHODPhenolic acids related to allelopathy effect in the soils around rhizosphere of R. glutinosa were determined by HPLC.

RESULTThe growth of R. glutinosa under normal rotation was strong. During harvest, the dry weight of the root tube and the volume of the R. glutinosa under normal rotation were 6.02 and 7.71 times of the ones under successive cropping.

CONCLUSIONThe seeding stage and elongating stage are the crucial periods for the autotoxic effect of the R. glutinosa under continuous cropping. During these periods, the content of coumaric acid, 4-hydroxybenzoic acid, syringic acid, and ferulic acid of R. glutinosa under successive cropping are notably negative correlation with the growth of the leaf and root tuber of R. glutinosa under successive cropping. Among them, ferulic acid plays a major role in the restriction effect on R. glutinosa under successive cropping.

Chromatography, High Pressure Liquid ; Coumaric Acids ; analysis ; metabolism ; toxicity ; Gallic Acid ; analogs & derivatives ; analysis ; metabolism ; toxicity ; Hydroxybenzoates ; analysis ; metabolism ; toxicity ; Plant Leaves ; drug effects ; growth & development ; Plant Roots ; drug effects ; growth & development ; Rehmannia ; drug effects ; growth & development ; metabolism ; Soil ; analysis

OBJECTIVETo study the effects of continuous cropping obstacles on growth of Rehmannia glutinosa.

METHODThe growth indexes, activity of root ATPase, root activity and mineral nutritional absorption were determined.

RESULTContinuous copping decreased growth rate and declined the size of leaves. Activity of root ATPase and root activity were also inhibited.

CONCLUSIONThe deficiency of source capacity is an important factor to restrain the root development of R. glutinosa with continuous cropping, the decrease of root activity and ATPase activity as well as nutritional stress of potassium and nitrogen are the reasons for the effects of continuous cropping on the growth and development of R. glutinosa.

Absorption ; Adenosine Triphosphatases ; metabolism ; Plant Roots ; enzymology ; growth & development ; metabolism ; Rehmannia ; enzymology ; growth & development ; metabolism ; Stress, Physiological