Objective To investigate the correlation between glycosylated hemoglobin (HbAlc) and carotid intima-media thickness (CIMT) in non-diabetic peritoneal dialysis patients.Methods Forty-two non-diabetic peritoneal dialysis adult patients were enrolled in this study [mean age was (48.2± 12.3) years,50％ was male].CIMT was determined by carotid ultrasound.Patients were divided into two groups according to CIMT:CIMT normal group (CIMT ＜ 0.9 mm) and CIMT thickening group (CIMT≥0.9 mm).HbAlc,2-hour postprandial blood glucose (2hPBG) and other factors of the patients were analyzed with Spearman rank correlation and multiple linear regression.Results CIMT was correlated with age,2hPBG,LDL-C,TG,TC,HbA1c in non-diabetic peritoneal dialysis patients (r =0.355,0.373,0.416,0.345,0.351,0.456,all P ＜ 0.05).Multiple linear regression showed that HbA1c was the most powerful influence factor of CIMT(β =0.459).Conclusion HbA1c level is positively correlated with CIMT and may be a predictor of carotid atherosclerosis in non-diabetic peritoneal dialysis patients.

Objective To explore the clinical diagnostic value of plasma homocysteine (Hcy)in the prognosis of patients with acute cerebral infarction (ACI).Methods According to Hcy median value on admission,78 ACI patients with hyperhomocysteinemia were divided into high-Hcy group and low-Hcy group,and meanwhile 34 ACI patients with normal Hcy level were de-signed as control group.All patients were given the secondary prevention for cerebrovascular dis-ease.On the basis of conventional therapy,high-Hcy group and low-Hcy group were treated with vitamin B12.BI and NIHSS scores were respectively evaluated,and Hcy level was detected in 3 groups on first day of admission and 21st day of treatment,and the clinical efficiency was compared between high-Hcy group and low-Hcy group.Results Hcy levels in high-Hcy group and low-Hcy group decreased significantly on 21st day of treatment.Hcy levels in high-Hcy grouy and low-Hcy group were significantly higher than control group on first day of admission and 21st day of treat-ment,and the increasing range in high-Hcy group was even larger.NIHSS scores decreased,while BI scores increased significantly in 3 groups on 21st day of treatment.NIHSS scores in high-Hcy group and low-Hcy group were significantly higher than control group on first day of admission and 21st day of treatment,while BI scores were lower,and the increasing and decreasing ranges in high-Hcy group were even larger.The overall response rate of low-Hcy group was superior to high-Hcy group.Conclusion Detecting Hcy level can predict the prognosis of patients with ACI,and reduc-ing Hcy level can improve the neural functional defects and life capability of patients.

Objective To explore the clinical diagnostic value of plasma homocysteine (Hcy)in the prognosis of patients with acute cerebral infarction (ACI).Methods According to Hcy median value on admission,78 ACI patients with hyperhomocysteinemia were divided into high-Hcy group and low-Hcy group,and meanwhile 34 ACI patients with normal Hcy level were de-signed as control group.All patients were given the secondary prevention for cerebrovascular dis-ease.On the basis of conventional therapy,high-Hcy group and low-Hcy group were treated with vitamin B12.BI and NIHSS scores were respectively evaluated,and Hcy level was detected in 3 groups on first day of admission and 21st day of treatment,and the clinical efficiency was compared between high-Hcy group and low-Hcy group.Results Hcy levels in high-Hcy group and low-Hcy group decreased significantly on 21st day of treatment.Hcy levels in high-Hcy grouy and low-Hcy group were significantly higher than control group on first day of admission and 21st day of treat-ment,and the increasing range in high-Hcy group was even larger.NIHSS scores decreased,while BI scores increased significantly in 3 groups on 21st day of treatment.NIHSS scores in high-Hcy group and low-Hcy group were significantly higher than control group on first day of admission and 21st day of treatment,while BI scores were lower,and the increasing and decreasing ranges in high-Hcy group were even larger.The overall response rate of low-Hcy group was superior to high-Hcy group.Conclusion Detecting Hcy level can predict the prognosis of patients with ACI,and reduc-ing Hcy level can improve the neural functional defects and life capability of patients.

Depsides and depsidones have attracted attention for biosynthetic studies due to their broad biological activities and structural diversity. Previous structure‒activity relationships indicated that triple halogenated depsidones display the best anti-pathogenic activity. However, the gene cluster and the tailoring steps responsible for halogenated depsidone nornidulin ( 3) remain enigmatic. In this study, we disclosed the complete biosynthetic pathway of the halogenated depsidone through in vivo gene disruption, heterologous expression and in vitro biochemical experiments. We demonstrated an unusual depside skeleton biosynthesis process mediated by both highly-reducing polyketide synthase and non-reducing polyketide synthase, which is distinct from the common depside skeleton biosynthesis. This skeleton was subsequently modified by two in-cluster enzymes DepG and DepF for the ether bond formation and decarboxylation, respectively. In addition, the decarboxylase DepF exhibited substrate promiscuity for different scaffold substrates. Finally, and interestingly, we discovered a halogenase encoded remotely from the biosynthetic gene cluster, which catalyzes triple-halogenation to produce the active end product nornidulin ( 3). These discoveries provide new insights for further understanding the biosynthesis of depsidones and their derivatives.