AIM: To analyse the main chemical constituents of Shuanghuanglian Injection Power(Flos lonicerae japanicae,Radix scutellariae and Fructus forsythiae) by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS: The sample solution was analysed by a Zorbax C_18 column with a gradient mobile phase comprised of acetonitrile and water.Electrospray ionization mass spectrometry detector was used,full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS: To analyse the mass spectrum of every liquid chromatography peaks,we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder,9 compounds were identified,including quinic acid,caffeic acid,three monocaffeoylquinic acids,dicaffeoylquinic acid,forsythin,forsythoside and baicalin. CONCLUSION: Quinic acid is identified from Shuanghuanglian Injection Power for the first time.High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.

OBJECTIVE:To study the lung targeted characteristic of tetrandrine polylactic acid microsphere(TPM).MET_HODS:TPM and tetrandrine injection were injected intravenously into mouse,an RP-HPLC was established to measure the content of TPM in biological samples,and the concentrations of tetrandrine in different tissues of mice were determined and compared.RESULTS:The determinable concentration of tetrandrine in plasma was in the linear range of 0.519～17.000?g/ml(r=0.9 996),the recovery was 97.32%,the mean value of RSD was 4.46%.After the use of TPM,the concentrations of tetrandrine in mouse tissues were significantly higher than tetrandrine injection,and the highest concentration was detected in lungs of mouse.CONCLUSIONS:TPM is distinguishingly lung targeted.

OBJECTIVE:To provide scientific evidence for the microsphere's m etabolism in vivo of lung targeted tetran?drine polylactic acid(TET-PLA)microspheres.METHODS:Blood was sampled from carotid artery after microspheres were injected into rabbit through auricular vein,the concentration of TET in plasma was determined by RP-HPLC method;The pharmacokinetic parameters were obtained by using3p87program.RESULTS:Concentration-time curves of TET micro?spheres were fitted to a2-compartment model with t 1/2? of(286.49?237.55)min and AUC of(810.33?287.49)(?g?min)/ml.CONCLUSION:TET-PLA microspheres show sustained release effects in rabbits.

OBJECTIVE:To establish phenol-sulfuric acid method for the determination of the content of total polysaccharides in Weile granules.METHODS:The samples were deproteinized by Sevag method,colored by phenol-sulfuric acid method(water bath at 60 ℃ for 30 min),and the content of total polysaccharides in Weile granules was determined with Glucose as control.RESULTS:The linear range of Glucose was 20.47～102.36 ?g?mL-1(r=0.999 4),with an average recovery of 99.09%(RSD=0.92%,n=6).The content of total polysaccharides in Weile granules was 41.63%.CONCLUSION:This method was simple,accurate and reproducible,and suitable for quality control of Weile granules.

Objective To develop a GC-MS method for the determination of ephedrine in human plasma and study the pharmacokinetic parameters of the ephedrine in different compatibility of the minister, adjuvant, and guide herbs to the monarch herb in Mahuang Decoction. Methods Orthogonal design was used to determine eight compatible groups from Mahuang Decoction (MHD). The healthy male volunteers were divided into groups in random, each group concluding eight men. After taking the medicine orally, venous blood would be taken out at different times. Determining ephedrine in plasma and plotting the concentration-time curve, the pharmacokinetic parameters of each compatible group were calculated by WinNonlin 4.0.1. The statistical analysis of the pharmacokinetic parameters were proceeded by SPSS 10.0. Results All concentration-time curves were adequately modeled by one compartment, first order absorption model, and no lag time. Some parameters of ephedrine showed significant variance (P

Objective To study the influence of Mahuang Decoction(MD)in different combinations on diaphoretic function in rats.Methods Vacuole incidence of axillary sweat gland was determined by observing the histolomorphological changes of vacuole to evaluate the diaphoretic function.The diaphoretic function was observed in 30 minutes after administration.Results The combination of Mahuang(Herba Ephedrae)and Guizhi(Ramulus Cinnamomi)had the strongest diaphoretic function in all of the different combinations.The diaphoretic function of the combinations including Mahuang(Herba Ephedrae)was stronger than those excluding Mahuang(Herba Ephedrae).The diaphoretic function of Mahuang(Herba Ephedrae)became stronger when used together with Guizhi(Ramulus Cinnamomi),remained the same when used together with Xingren(Semen Armeniacea Amarum),and became weaker when used together with Gancao(Radix Glycyrrhizac).Conclusion Mahuang(Herba Ephedrae),Guizhi(Ramulus Cinnamomi),Xingren(Semen Armeniacea Amarum),and Gancao(Radix Glycyrrhizac)play the roles of principle drug,assistant drug,adjuvant drug and conductantr drug respectively in Mahuang Decoction,which embodying the method of composing a prescription.

AIM: To investigate the chemical constituents of Shuanghuanglian Injection(Flos lonicerae japonicae, Radix scutellariae, Fructus forsythiae). METHODS: Chromatography and spectral analysis were used respectively to isolate and identify the constituents. RESULTS: Six compounds were isolated from Shuanghuanglian Injection which included baicalin(Ⅰ)、quercitrin(Ⅱ)、hyperoside(Ⅲ)、rutin(Ⅳ)、quercetin(Ⅴ) and luteolin(Ⅵ). CONCLUSION: The other five compounds were first isolated and identified in Shuanghuanglian Injection except baicalin(Ⅰ).

Objective To discuss the influence of drug compatibility on the components of the volatile oil in Flos Lonicerae and Fructus Forsythiae. Methods GC-MS system was used to analyze the volatile oil in Flos Lonicerae, Fructas Forsythiae and their combination. The effect of compatibility on the components and content of volatile oil was evaluated. Results The results of GC-MS showed that gerani]o, tetradecanoic acid methyl ester, hexadecanoic acid ethyl ester, fluoranthene, 9, 12, 15-Octadeeatrienoic acid ethyl ester and linoleic acid was detectable in Flos Lonicerae but was undetectable in the drug pair; camphene, alpha-terpinene, perillaalcohol, 2-beta-pinene, beta-hexadecanal, phellandrene was de-tectable in Fructus Forsythiae but was undetectable in the drug pair; limonene, isolongifolene, nonadecane was detectable in the drug pair but was undetectable in the two single drugs. Conclusion The compatibility of the drug pair has an effect on components and contents in the volatile oil of Flos Lonicerae and Fructus Forsythiae.

Objective To establish the HPLC fingerprints of Radix Glycyrrhizae. Methods The RP-HPLC method was used, Zorbax SB C_ 18 column (250 mm?4.6 mm, 5 ?m) was employed; the acetonitrile-1.2% acetic acid (gradient elution) was used as mobile phase, analytic time was 70 min, and detective wavelength was at 254 nm. Results The HPLC fingerprints of Radix Glycyrrhizae were set up. The result showed that 38 peaks were common in different sources. The results of method validation met technical standard of fingerprints. Conclusion The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Radix Glycyrrhizae.