AIM: To analyse the main chemical constituents of Shuanghuanglian Injection Power(Flos lonicerae japanicae,Radix scutellariae and Fructus forsythiae) by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS: The sample solution was analysed by a Zorbax C_18 column with a gradient mobile phase comprised of acetonitrile and water.Electrospray ionization mass spectrometry detector was used,full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS: To analyse the mass spectrum of every liquid chromatography peaks,we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder,9 compounds were identified,including quinic acid,caffeic acid,three monocaffeoylquinic acids,dicaffeoylquinic acid,forsythin,forsythoside and baicalin. CONCLUSION: Quinic acid is identified from Shuanghuanglian Injection Power for the first time.High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.

OBJECTIVE:To study the lung targeted characteristic of tetrandrine polylactic acid microsphere(TPM).MET_HODS:TPM and tetrandrine injection were injected intravenously into mouse,an RP-HPLC was established to measure the content of TPM in biological samples,and the concentrations of tetrandrine in different tissues of mice were determined and compared.RESULTS:The determinable concentration of tetrandrine in plasma was in the linear range of 0.519～17.000?g/ml(r=0.9 996),the recovery was 97.32%,the mean value of RSD was 4.46%.After the use of TPM,the concentrations of tetrandrine in mouse tissues were significantly higher than tetrandrine injection,and the highest concentration was detected in lungs of mouse.CONCLUSIONS:TPM is distinguishingly lung targeted.

OBJECTIVE:To provide scientific evidence for the microsphere's m etabolism in vivo of lung targeted tetran?drine polylactic acid(TET-PLA)microspheres.METHODS:Blood was sampled from carotid artery after microspheres were injected into rabbit through auricular vein,the concentration of TET in plasma was determined by RP-HPLC method;The pharmacokinetic parameters were obtained by using3p87program.RESULTS:Concentration-time curves of TET micro?spheres were fitted to a2-compartment model with t 1/2? of(286.49?237.55)min and AUC of(810.33?287.49)(?g?min)/ml.CONCLUSION:TET-PLA microspheres show sustained release effects in rabbits.

AIM: To investigate the chemical constituents of Shuanghuanglian Injection(Flos lonicerae japonicae, Radix scutellariae, Fructus forsythiae). METHODS: Chromatography and spectral analysis were used respectively to isolate and identify the constituents. RESULTS: Six compounds were isolated from Shuanghuanglian Injection which included baicalin(Ⅰ)、quercitrin(Ⅱ)、hyperoside(Ⅲ)、rutin(Ⅳ)、quercetin(Ⅴ) and luteolin(Ⅵ). CONCLUSION: The other five compounds were first isolated and identified in Shuanghuanglian Injection except baicalin(Ⅰ).

OBJECTIVE:To establish phenol-sulfuric acid method for the determination of the content of total polysaccharides in Weile granules.METHODS:The samples were deproteinized by Sevag method,colored by phenol-sulfuric acid method(water bath at 60 ℃ for 30 min),and the content of total polysaccharides in Weile granules was determined with Glucose as control.RESULTS:The linear range of Glucose was 20.47～102.36 ?g?mL-1(r=0.999 4),with an average recovery of 99.09%(RSD=0.92%,n=6).The content of total polysaccharides in Weile granules was 41.63%.CONCLUSION:This method was simple,accurate and reproducible,and suitable for quality control of Weile granules.

Objective To develop a GC-MS method for the determination of ephedrine in human plasma and study the pharmacokinetic parameters of the ephedrine in different compatibility of the minister, adjuvant, and guide herbs to the monarch herb in Mahuang Decoction. Methods Orthogonal design was used to determine eight compatible groups from Mahuang Decoction (MHD). The healthy male volunteers were divided into groups in random, each group concluding eight men. After taking the medicine orally, venous blood would be taken out at different times. Determining ephedrine in plasma and plotting the concentration-time curve, the pharmacokinetic parameters of each compatible group were calculated by WinNonlin 4.0.1. The statistical analysis of the pharmacokinetic parameters were proceeded by SPSS 10.0. Results All concentration-time curves were adequately modeled by one compartment, first order absorption model, and no lag time. Some parameters of ephedrine showed significant variance (P

Objective To study the influence of Mahuang Decoction(MD)in different combinations on diaphoretic function in rats.Methods Vacuole incidence of axillary sweat gland was determined by observing the histolomorphological changes of vacuole to evaluate the diaphoretic function.The diaphoretic function was observed in 30 minutes after administration.Results The combination of Mahuang(Herba Ephedrae)and Guizhi(Ramulus Cinnamomi)had the strongest diaphoretic function in all of the different combinations.The diaphoretic function of the combinations including Mahuang(Herba Ephedrae)was stronger than those excluding Mahuang(Herba Ephedrae).The diaphoretic function of Mahuang(Herba Ephedrae)became stronger when used together with Guizhi(Ramulus Cinnamomi),remained the same when used together with Xingren(Semen Armeniacea Amarum),and became weaker when used together with Gancao(Radix Glycyrrhizac).Conclusion Mahuang(Herba Ephedrae),Guizhi(Ramulus Cinnamomi),Xingren(Semen Armeniacea Amarum),and Gancao(Radix Glycyrrhizac)play the roles of principle drug,assistant drug,adjuvant drug and conductantr drug respectively in Mahuang Decoction,which embodying the method of composing a prescription.

Objective To establish a method to determinate polysaccharides in Hedyotic diffusa, then compare the polysaccharides content in Hedyotic diffusa form Jiangxi, Guangdong, Guangxi. Methods Sulfuric acid -phenol coloration were applied to analysis polysaccharides content. The detective wave length was 486 nm. Results Polysaccharides in Hedyotic diffusa from Jiangxi province was a little higher than the others. Conclusion Polysaccharides content in Hedyotic diffusa form different habitat are almost alike.

AIM:To determine icatiin,psoralen and isopsoralen in Yishen Buxue Capsule(Herba Epimedii;Fructus Psoraleae;Sanguis Draconis;Cortex Eucommiae,etc)by PR-HPLC.METHODS:The determination of icariin was carried out by HPLC with a Agilent HC-18(150 mm?4.6 mm,5 ?m)column,acetonitrile-water(30∶70)was as a mobile phase,detection wavelength was at 270 nm;With the same Agilent HC-18 column for psoralen and isopsoralen,the mobile phase was methanol-water(52∶48),detection wavelength was at 246 nm.RESULTS:Icariin showed a good linear relationship at the range of 0.110 4-0.883 2 ?g(r2= 0.999 8),the avearge recovery of icariin was 97.49%(RSD=1.47%,n=6).Psoralen also showed a good linear relationship at the range of 0.041 04-0.451 44 ?g(r2= 0.999 0),the average recovery for it was 104.51%(RSD= 1.58%,n=6);Isopsoralen showed a good linear relationship at the range of 0.037 52 ?g-0.412 72 ?g(r2= 0.999 9),the average recovery of isopsoralen was 103.01%(RSD=1.01%,n=6).CONCLUSION:The methods are sensitive,accurate,reproducible,specific and can be used for quality control of Yishen Buxue Capsule.