In general the valuation criterions of teaching quality in class are exactness,lifelikeness and profundity.The author's own understanding and some suggestions about the criterions will be put forward in this article.

With the advances of medical management and surgical techniques during cardiopulmonary bypass,children with congenital heart disease are increasingly surviving to adulthood.Research into their neurodevelopmental outcomes in the past 20 years had revealed sobering realizations about the adverse impact on the quality of life of these surgical survivors.A multitude of factors contribute to the incidence and severity of neurodevelopmental abnormalities and can be separated into preoperative,intraoperative,and postoperative events,in addition to genetic contributions and socioeconomic factors.This article highlight some of current understanding of these protean factors.Understanding should lead to modification of current techniques and management strategies,all with the ultimate goal of improving patients' quality of life.

Actinomyces ; growth & development ; Coinfection ; Humans ; Maxillary Sinus ; Maxillary Sinusitis ; diagnosis ; microbiology

Objective: To express and purify the inhibitor of differentiation(Id3) in E.coli.Methods: The DNA fragment in the coding region of the human Id3 gene was amplified by PCR and cloned into the pGEM-T Easy vector for sequencing.The Id3 cDNA fragment was then subcloned into the prokaryotic expression vector(pET-32a)(+) and transformed into E.coli BL 21(DE3).The expression of the histidine-tagged(His-Tag) fusion protein was induced with isopropy-?-D-thiogalactoside(IPTG),confirmed by Western blot and purified by the immobilized Ni2+ absorption chromatographic column.Results: The prokaryotic expression vector of Id3 was successfully constructed.Western blot confirmed the expression of the His-Tag fusion protein in E.coli BL 21(DE3) and that of the Id3 fusion protein with the relative molecular mass size of 33 000 after purified by the affinity chromatographic column. Conclusion: Recombinant Id3 can be expressed in E.coli BL 21(DE3) and the obtained fusion protein can be purified by the Ni-affinity chromatography column.

Adult ; Female ; Hearing Loss, Noise-Induced ; diagnosis ; etiology ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Occupational Diseases ; diagnosis ; etiology

Altitude Sickness ; Chronic Disease ; Humans ; Male ; Middle Aged ; Occupational Diseases

Acupuncture Points ; Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Vasculitis ; therapy

Integrins such as αvβ3, α5β1 play a key role in angiogenesis regulation, invasion and metastasis, inflammation, wound healing, etc. The up-regulation of integrin αvβ3 after cerebral ischemic stroke can promote angiogenesis, which in turn improves functional recovery. In addition, the integrin αvβ3 inhibitor can block the blood-brain barrier (BBB) leakage induced by vascular endothelial growth factor (VEGF) and also can reduce inflammatory reaction, decrease the deposition of fibrinogen. Other studies showed that integrin αvβ3 is not essential in revascularization. Therefore, the effect of integrin αvβ3 in the whole process of brain function recovery merits further study.

BACKGROUND:Studies demonstrated that matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinases-1(TIMP-1) exhibit important role in corneal defect of rats with alkali burns.OBJECTIVE:To observe the promoting effect of recombinant human epidermal growth factor(rhEGF) on rats' corneas following alkali burns,in addition,to explore the effect and significance of rhEGF on the expression of MMP-1 and TIMP-1 in alkali burned rats.DESIGN,TIME AND SETTING:A randomized controlled animal experiment was performed at the Laboratory Animal Center,Liaoning Medical College from March 2008 to May 2009.MATERIALS:Sixty healthy SD rats,weighting 180-210 g,with 6-8 weeks old,half male and half female.All rats were confirmed that no anterior segment disease before the experiment with slit lamp microscope examination.METHODS:Sixty rats were randomly divided into experimental and control groups,with 30 animals in each group.After the preparation of corneal alkali burn models,cornea of rats in the experimental and control groups were given rhEGF eye drops,and physiological saline eye drops respectively,fourth a day,consecutive for 21 days.Six rats of each group were killed at days 1,4,7,14 and 21 after operation.MAIN OUTCOME MEASURES:①Changes of cornea after alkali burned was observed by slit lamp microscope.②The expressions of MMP-1 and TIMP-1 in cornea were detected with immunohistochemistry and computer image analysis system at different time points.RESULTS:The slit lamp microscope results showed that the area of corneal ulcer in the experimental group was obvious smaller than the control group at the corresponding time points.The expression of MMP-1 gradually increased and reached a peak level at 14 days after surgery,then began to decrease.TIMP-1 expressed in the early phrase,but later,its activity gradually decreased,reached the lowest level at 14 days,then began to increase,and peaked at 21 days.The level of MMP-1 in the experimental group was greatly lower than that in the control group at each time points.However,the level of TIMP-1 of the experimental group was greatly higher compared with the control group.CONCLUSION:The rhEGF eye drops can improve effectively the healing of corneal epithelial defect by adjusting the proportion of matrix metalloproteinase and its tissue inhibitor.

Objective To observe the effect of mild hypothermia on expression of vascular endothelial growth factor (VEGF) and hypox-ia-inducible factor-1α(HIF-1α) in brain tissues in rats with cerebral ischemia-reperfusion. Methods Thirty Sprague-Dawley rats were ran-domly divided into sham group, model group and mild hypothermia group with ten rats in each group. The latter two groups were estab-lished middle cerebral artery occlusion (MCAO) for two hours and reperfusion model. The mild hypothermia group received mild hypother-mia treatment for 24 hours. The neurologic function was evaluated with Longa's score. The brain water content was measured by wet/dry method. The infarction area ratio was assessed by TTC staining. The protein levels of VEGF and HIF-1αwere determined by ELISA, while the mRNA level of VEGF was assessed by qPCR. Results Compared with the sham group, the score of neurologic score, the brain water content ratio and the infarction area ratio, the expression of VEGF protein and HIF-1αprotein, and the mRNA level of VEGF increased in the model group (P<0.05). Compared with the model group, the score of neurologic score significantly decreased (χ2=26.657, P<0.001), the brain water content ratio and the infarction area ratio decreased (P<0.05), and the expression of VEGF protein and HIF-1αprotein, and the mRNA level of VEGF further increased (P<0.05) in the mild hypothermia group. Conclusion Mild hypothermia may protect against isch-emic-induced neurologic injury by up-regulating the expression of VEGF and HIF-1α, and promote the angiogenesis in the rats with cerebral ischemia-reperfusion.