Objective To observe the rat tracheal stem cells in situ.Methods Tracheal rings of Wistar rats treated with 5-FU to make an injure model in vitro then observing the process of regeneration in sequence time from 3*!hours to 48*!hours. Use light microscope to detect the development of tracheal epithelium,assay the level of proliferating cell nuclear antigen (PCNA) immunohistochemistry and perform Hoechst33342 staining to search the negative staining cells. Results Twelve hours after 5-FU it could be seen that tracheal mucosa exfoliated with nuke-nucleus like cells nailed just above the basement membrane,6*!hours after removing 5-FU we could see that the tracheal rings were covered with squamous epithelium. It could be seen that PCNA immunohistochemistry negative staining cells located bordering on the positive one. Meanwhile under fluorescent contrast microscope negative cells with no Hoechst dye combinations were among most of the positive cells. 12*!hours after removing 5-FU mucous granular cells and cilia cells could be seen. After 48*!hours the whole tracheal rings were covered with pseudostratified columnar ciliated epithelium.Conclusion 5-FU is a cell-cycle specific antimetabolite cytotoxicant.It can make the cycling cells degeneration and necrosis but has little effect on the G 0 cells. Few G 0 cells which look like nuke nucleus cells randomly on the basement have the ability to efflux the dye Hoechst33342.The tracheal rings are regenerated through these cells differentiation and proliferation.

Objective To evaluate the application of diffusion-weighted MR imaging in hepatic echinococcosis.Methods Diffusion-weighted MR imaging and measurement of apparent diffusion coefficient (ADC)were performed in 23 patients with 30 hepatic echinococcosis(cystic echinococcosis 24, alveolaris echinococcosis 6).Results The mean ADC values of cystic echinococcosis, alveolaris echinococcosis and normal liver were (3.50?0.35)?10 -3 mm2/s,(2.22?0.22)?10 -3 mm2/s and (1.41?0.09)?10 -3 mm2/s respectively.The difference between the mean ADC values was significant(?

Objective:To prepare the recombinant neuron protective protein TAT-Bcl-XL in E.coli expression system and to determine its anti-apoptosis activity.Methods:DNA fragment of TAT-Bcl-XL was obtained by RT-PCR using primers that were specific for Bcl-XL gene. Prokaryotic expression vector(pTBTOPO) was constructed by insertion of TAT-Bcl-XL DNA fragment into pCRT7/CT-TOPO vectors, and the recombinant protein was detected by SDS-PAGE and Western blot analysis using anti-V5 tag epitope antibody as the primary antibody. The recombinant fusion protein was purified by affinity chromatography, and the anti-apoptosis function of the purified protein was determined by flow cytometry.Results:A molecular weight of 30 000 protein was detectable in both SDS-PAGE and Western blot analysis. Immunofluorescent staining showed the fusion protein was distributed in cell plasma after incubation with 200 nmol/L fusion protein. Flow cytometry data indicated that the recombinant protein could enhance cell survival by 40% when 293T cells were treated with 250 ?mol/L zinc chloride as a supplementation in cell culture media.Conclusion:High level expression of the reported neuron protective protein was obtained in E.coli expression system, and the purified recombinant proteins remained its anti-apoptosis activity in our preliminary characterization.

Iron overload is a metabolic disorder characterized by excessive iron deposition in the liver,pancreas,heart,endocrine organs, etc.,resulting in structural damage and dysfunction.The liver is the primary organ for iron storage,and excessive iron deposition induces liver inflammation and fibrosis,which may progress to cirrhosis and even liver cancer,with a poor prognosis.Accurate evaluation and effec-tive treatment can reduce liver injury caused by iron overload and improve patients′survival.

Aged ; Amiodarone ; adverse effects ; Chemical and Drug Induced Liver Injury ; etiology ; Drug-Related Side Effects and Adverse Reactions ; Humans ; Male

To elucidate the processing mechanism of Herba Epimedii from the new view of the intestinal absorption and metabolism.From analyzing the literatures of processing mechanism and togethering with the research of our lab,a new idea of processing mechanism of Herba Epimedii was brought:the pharmacologically active and easier absorbed flavonoids might be present more in the herbs when changing the heating processing parameters and thereby increased or maintained the efficacy.This thesis first pointed the new idea and method that the intestinal absorption and metabolism of herbs should be considered when studying the mechanism of processing.

Objective: To study the difference in tooth profile among Chinese,Caucasian and Japanese with normal occlusion.Methods: The data of the measurments of Chinese,Caucasian and Japanese with normal occlusion were collected from published studies.Crown angulation,crown inclination and crown convex were used in the comparation among the peoples. Results: Tooth profile in Chinese was significantly different from that of Caucasian but was similar to that of Japanese. Conclusion: The orthodontic appliances should be modified when we use the straight wire appliances that is suitable for Caucasian and Japanese.

Objective To discuss the cause,prevention,treatment of airway anastomosis stenosis after lung transplantation.Methods From Jan.2003 to Sep.2005,8 homogeneity variant single lung transplantations were performed successfully at our hospital.All the cases were subjected to a running suture on the membranous wall and single stitches on the anterior cartilaginous part.Among the 11 cases,9 were still alive well. In 4 patients having aspergillus infection after operation,2 patients suffered from bronchial anastomosis stenosis.Results There were bronchial anastomosis stenosises in the 2 patients one month and 7 months after transplantation, respectively.All of them were cured by stenting.Conclusions Airway anastomosis stenosis was associated with bronchial ischemia,aspergillus infection,suture technique and so on.The stenting was positively effective for airway anastomosis stenosis.

Objective To investigate the effect of artorvastatin on macrophage accumulation in tubulointerstitium of unilateral ureteral obstructive (UUO) nephropathy and its possible mechanism.Methods Twenty-one rats were randomly divided into three groups: sham-operatipn, UUO, UUO+ artorvastatin. Immunohistochemistry staining of CD68 and M-CSF was used to define the macrophage accumulation and expression of interstitial M-CSF. Lipid profile in these groups was also determined. Results CD68 + cells and M-CSF expression were significantly increased at day 10 after UUO operation, this kind of CD68 + cell accumulation and M-CSF expression up-regulation were ameliorated by artorvastatin treatment. In UUO and atorvastatin treated groups, the number of macrophage was positively correlated with tubulointerstitial M-CSF expression. There was no significant difference about serum lipid among the three groups. Conclusion Atorvastatin can reduce interstitial macrophage accumulation in UUO nephropathy. This therapeutic effect might relate to down-regulation of tubulointerstitial M-CSF expression.

Objective:To investigate the effect of transcatheter arterial chemoembolization (TACE) on cell proliferation in hepatocellular carcinoma(HCC).Methods:Expression of proliferating cell nuclear antigen (PCNA) was detected immunohistochemically in paraffinmbedded specimen of 65 HCCs.Results:PCNA labeling index was significantly lower in the group of HCCs who underwent TACE preoperatively (35.83?12.23% vs 59.92?23.57%,P