Objective To explore the effect and safety of the regimen containing Moxifloxacin in intensive phase for pulmonary tuberculosis patients. Methods 88 cases with initial tuberculosis(each group of 44 cases) were studied in comparison in the same period. The treatment group contained isoniazid, rifampicin and moxifloxacin of antituberculosis regime and the control group contained isoniazid,rifampicin and pyrazineamide. Results After 2 months intensive phase anti-tubercular treatment ,the sputum negative conversion rates was 95.0% and control's was 94.4%(P ＞ 0.05); the absorb rate of lesions: the treatment group 77.3 % and the control group 79.5%; the liver damage rate: the treatment group and control group was 68.2% ,34.1% respectively(P ＜ 0.05). Conclusion Anti-tuberculosis intensive phase regimes containing moxifloxacin for tuberculosis treatment was safe and effective in initial treatment of pulmonary tuberculose.

Objective:To obtain human S100A2-GST fusion protein for further research on human S100A2(hS100A2)protein function and its interactions with other proteins and systems.Methods:hS100A2 gene from pHAHA-hS100A2 was subcloned into an expression vector pGST-moluc to construct pGST-moluc-hS100A2.The new plasmid was identified by digestion with XhoⅠand EcoRⅠ.The recombinant BL21 was induced with IPTG to express recombinant fusion protein hS100A2-GST,which was purified by Glutathion-Sepharose 4B ball beads,identified by SDS-PAGE and Western Blot,and quantified by Bradford method.Results:After the digestion,the recombinant plasmid was cutted into two fragments,300 bp and 5kb.After treated with IPTG,the recombinant BL21 strain expressed a protein,which was about 36 kD and was recognized specifically by an-ti-hS100A2.Its yield was 5 mg/L bacterial culture.After isolated by Glutathion-Sepharose 4B ball beads,its purity was 92%.Conclusion:hS100A2-GST expression plasmid pGST-moluc-hS100A2 was constructed successfully.hS100A2-GST fusion pro-tein could be expressed in Escherichia coli with higher yield and isolated with higher purity,which lays the foundation for the follow-up of the hS100A2 research.

0.05). The patients whose Pre-S2 antigen and antibody are positive in the serum are mainly with chronic hepatitis B (P

OBJECTIVE:To compare the contents of Rhodioloside and polysaccharide extracted from different part in cultivated Rhodiola sachalinensis.METHODS:The Rhodioloside was extracted with 70% alcohol and determined by HPLC;The polysaccharide was extracted with water and determined by sulphuric acid-phenol method.RESULTS:The content of the Rhodioside ranged between 0.026 5% and 0.443 2%,and the content of polysaccharide between 0.965% and 4.612% in the medicinal herbs,with the main root showing the highest contents.CONCLUSION:The contents of Rhodioside and polysaccharide are obviously different in different part of cultivated Rhodiola sachalinensis.The results provided reasonable theoretical evidence for the exploitation of cultivated Rhodiola sachalinensis.

As a member of GTPase Rab family,Rab10 protein is not only involved in vesicle formation,transport,anchoring and fusion process,but also affects the occurrence and development of tumors.Research about the mechanisms of Rab10 in intracellular vesicle transport and tumor may provide a potential target and new idea for the anti-cancer therapy.

Biomedical Research ; Humans ; Multicenter Studies as Topic ; Vestibular Diseases ; diagnosis ; therapy

Ear Diseases ; congenital ; diagnosis ; surgery ; Ear, External ; abnormalities ; Ear, Middle ; abnormalities ; Humans

The effects of the temperature,materials and quantities of blood on results of PGM_1 phenotyping inbloodstains in a latin square were studied using polyacrylamide gel isoelectric focusing.The detectabletime of the bloodstains kept at 0℃,4℃,18℃ and 30℃ were 6 months,2 months,1 months and3 weeks respectively.The quantity of blood staining also had an effect on the detectable time of PGM_1subtyping in bloodstains.However,there was not any significant difference of detectable time of PGM_1subtyping in bloodstains on different materials was observed.In addition,a method for permanent storageof zymogram was developed using polyester sheet.

With the diversified development in training clinical students, an impeccable system in clinical on - job postgraduates training has been established. lt has been being an important problem for colleges and tutors to improve the quality in the postgraduates training program in the specialty of ophthalmology. This paper analyzed the problems in the training of clinical postgraduates and explored the ways to resolve the problems.

Objective:To analyze the causes and countermeasures for the inefficiency of implementing the contract community health service policy.Methods:Based on the authority and regulation of organization,it innovatively used the French organization theory,build an analysis framework of organizational actors adapting to the general organization.On the above basis combined with the characteristics of contract health service,it constructed a new organizational structure of contract health service and analyzed the low efficiency of policy implementation from the three relations among the actors,the power and the rule.Results and Conclusion:It needed to focus on authority and rule to rationally division the authorities of actors,optimize the organizational structure,adjust the benefit relationship of actors;play the regulation role,implement the incentive and constraint mechanism to improve the execution efficiency of contract health service policy.