Actinomyces ; growth & development ; Coinfection ; Humans ; Maxillary Sinus ; Maxillary Sinusitis ; diagnosis ; microbiology

With the advances of medical management and surgical techniques during cardiopulmonary bypass,children with congenital heart disease are increasingly surviving to adulthood.Research into their neurodevelopmental outcomes in the past 20 years had revealed sobering realizations about the adverse impact on the quality of life of these surgical survivors.A multitude of factors contribute to the incidence and severity of neurodevelopmental abnormalities and can be separated into preoperative,intraoperative,and postoperative events,in addition to genetic contributions and socioeconomic factors.This article highlight some of current understanding of these protean factors.Understanding should lead to modification of current techniques and management strategies,all with the ultimate goal of improving patients' quality of life.

In general the valuation criterions of teaching quality in class are exactness,lifelikeness and profundity.The author's own understanding and some suggestions about the criterions will be put forward in this article.

Integrins such as αvβ3, α5β1 play a key role in angiogenesis regulation, invasion and metastasis, inflammation, wound healing, etc. The up-regulation of integrin αvβ3 after cerebral ischemic stroke can promote angiogenesis, which in turn improves functional recovery. In addition, the integrin αvβ3 inhibitor can block the blood-brain barrier (BBB) leakage induced by vascular endothelial growth factor (VEGF) and also can reduce inflammatory reaction, decrease the deposition of fibrinogen. Other studies showed that integrin αvβ3 is not essential in revascularization. Therefore, the effect of integrin αvβ3 in the whole process of brain function recovery merits further study.

Objective: To express and purify the inhibitor of differentiation(Id3) in E.coli.Methods: The DNA fragment in the coding region of the human Id3 gene was amplified by PCR and cloned into the pGEM-T Easy vector for sequencing.The Id3 cDNA fragment was then subcloned into the prokaryotic expression vector(pET-32a)(+) and transformed into E.coli BL 21(DE3).The expression of the histidine-tagged(His-Tag) fusion protein was induced with isopropy-?-D-thiogalactoside(IPTG),confirmed by Western blot and purified by the immobilized Ni2+ absorption chromatographic column.Results: The prokaryotic expression vector of Id3 was successfully constructed.Western blot confirmed the expression of the His-Tag fusion protein in E.coli BL 21(DE3) and that of the Id3 fusion protein with the relative molecular mass size of 33 000 after purified by the affinity chromatographic column. Conclusion: Recombinant Id3 can be expressed in E.coli BL 21(DE3) and the obtained fusion protein can be purified by the Ni-affinity chromatography column.

Adult ; Female ; Hearing Loss, Noise-Induced ; diagnosis ; etiology ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Occupational Diseases ; diagnosis ; etiology

Acupuncture Points ; Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Vasculitis ; therapy

Altitude Sickness ; Chronic Disease ; Humans ; Male ; Middle Aged ; Occupational Diseases

Objective To observe the effect of mild hypothermia on expression of vascular endothelial growth factor (VEGF) and hypox-ia-inducible factor-1α(HIF-1α) in brain tissues in rats with cerebral ischemia-reperfusion. Methods Thirty Sprague-Dawley rats were ran-domly divided into sham group, model group and mild hypothermia group with ten rats in each group. The latter two groups were estab-lished middle cerebral artery occlusion (MCAO) for two hours and reperfusion model. The mild hypothermia group received mild hypother-mia treatment for 24 hours. The neurologic function was evaluated with Longa's score. The brain water content was measured by wet/dry method. The infarction area ratio was assessed by TTC staining. The protein levels of VEGF and HIF-1αwere determined by ELISA, while the mRNA level of VEGF was assessed by qPCR. Results Compared with the sham group, the score of neurologic score, the brain water content ratio and the infarction area ratio, the expression of VEGF protein and HIF-1αprotein, and the mRNA level of VEGF increased in the model group (P<0.05). Compared with the model group, the score of neurologic score significantly decreased (χ2=26.657, P<0.001), the brain water content ratio and the infarction area ratio decreased (P<0.05), and the expression of VEGF protein and HIF-1αprotein, and the mRNA level of VEGF further increased (P<0.05) in the mild hypothermia group. Conclusion Mild hypothermia may protect against isch-emic-induced neurologic injury by up-regulating the expression of VEGF and HIF-1α, and promote the angiogenesis in the rats with cerebral ischemia-reperfusion.