Combined acupuncture-medication anesthesia refers to an anesthesia method by combining acupuncture and small dose of anesthetics, i.e. acupuncture plus local anesthesia, acupuncture plus epidural anesthesia, acupuncture plus general anesthesia, and so on, used in cardiac, chest, skull, throat, abdominal, and urinary operations. Clinical studies show that the combined acupuncture-medication anesthesia has integrated the advantage of acupuncture in regulating the function of internal organs and the advantage of drugs insedation, thus producing a better protective effect on important organs than dry drug anesthesia during and after operation. This article summarized the latest research progress of the combined acupuncture-medication anesthesia in protecting organs duringthe perioperative period of pneumonectomy, pointed out the research frontiers of the application of this anesthesia method in pneumonectomy, and was of valuable reference for the clinical study in the future.

Objective To observe the clinical efficacy of fire-needle plus round-sharp-needle acupuncture in treating primary trigeminal neuralgia.Method Sixty-two patients with primary trigeminal neuralgia were randomized into a treatment group and a control group, 31 cases in each group. The treatment group was intervened by fire-needle therapy at the trigger points followed by deep needling with round-sharp needle at Xiaguan (ST7), and the distal points Hegu (LI4) and Neiting (ST44) were also treated. The same acupoints were selected in the control group but treated with ordinary needling method. The clinical efficacies were evaluated after 2 treatment courses.Result The total effective rate was 93.5%(29/31) in the treatment group versus 77.4% (24/31) in the control group, and the difference was statistically significant (P<0.05).Conclusion Fire-needle plus round-sharp-needle acupuncture is an effective approach in treating primary trigeminal neuralgia.

The 210-layer consecutive CT Dicom format image of a healthy human body thoracolumbar was directly reading into the Mimics,and defining bone organization threshold,withdrawing each outline,partitioning each edge of layer picture,selectively editing and repairing by hole processing.After the redundancy data was cleaned,the thoracolumbar 3-D finite element geometry model was acquired.Then area mesh could be introduced into Ansys software directly to compartmentalize mesh body,and then transferred the body mesh to mimics to assignment on the basis of CT values,then add the ligaments and joint-bindings to perfect the 3D finite element model in Ansys.Then bound the lower of L_1 and impose displacement-load in the upper edge of T_(12).The 3D finite element model was reconstructed using CT images from a patient of T_(12) and L_1 vertebral compression fractures.After that,a realistic appearance and calculation accuracy 3-D bone finite element model of thoracolumbar was established.The results showed that reconstructing a three-dimensional finite element model of thoracolumbar spine compression fractures based on fracture mechanism by information from normal human is scientific and effective.

Objective To evaluate the causes of postoperative complications in severe liver rupture and explore the prevention experienees.Methods The clinical data of 66 liver rupture patients who underwent surgical treatment in our hospital from January 2005 to July 2012 were retrospectively analyzed.The commou complications after surgery and the treatment measures of the complications were sludied.Results There were 9 deaths of severe liver rupture after surgery and related complications were found in 22 cases,including bile leakage (5 cases),intrahepatic abscess (3 eases),subphrenic infection (3 cases),pleural effusion (8 cases),intra-abdominal hemorrhage (2 cases),stress ulcer bleeding (2 cases).Conclusions Using damage control theory could improve the cure rate.Precise liver transection and postoperative excellent management was able to reduce the incidence of postoperative complications in severe liver rupture.

Objective: To synthesize novel tetrahydroisoquinolines with both anti-fungal and contraceptive activities, so as to provide precursor structures for contraceptives with anit-fungal activities. Methods: 3,4-dimethoxyphenylethylamine was taken as the template and the title compounds were synthesized through Pictet-Spengler reaction, neutralization reaction, substitution, hydrolysis, and acylation. The anti-fungal activity and sperm-killing activity of the target compounds were tested in vitro. Results: Fourteen title compounds were obtained and they were: 2-octyl-6, 7-dimethyl-1, 2, 3, 4-tetrahydro-isoquinoline hydrochloride(1), 2-nonyl-6, 7-dimethyl-1,2, 3,4-tetrahydro-isoquinoline hydrochloride(2), 2-decyl-6, 7-dimethy-1, 2, 3, 4-tetrahydro-isoquinoline hydrochloride(3), 2-dodecyl-6, 7-dimethyl-1,2,3,4-tetrahydro-isoquinoline hydrochloride(4), 2-dodecyl 6, 7-diacetoxy-1, 2,3,4-tetrahydro-isoquinoline hydrochloride(5), 2-pentyl-6,7-diacetoxy-1,2,3,4-tetrahydro-isoquinoline hydrobromide(6), 2-hexyl-6, 7-diacetoxy-1,2,3,4-tetrahydro-isoquinoline hydrobromide(7), 2-heptyl-6, 7-diacetoxy-1,2,3,4-tetrahydro isoquinoline hydrobromide (8), 2-octyl-6, 7-dimethyl-1, 2, 3, 4-tetrahydro-isoquinoline hydrobromide(9), 2-nonyl-6, 7-dimethyl-1, 2, 3, 4-tetrahydro-isoquinoline hydrobromide(10), 2-decyl-6, 7-dimethy-1, 2, 3, 4-tetrahydro-isoquinoline hydrobromide(11), 2-dodecyl-6, 7-dihydroxyl 1, 2, 3, 4-tetrahydro-isoquinoline hydrobromide(12), 2-tetradecyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydro-isoquinoline hydrobromide(13), and 2-cetyl-6, 7-dihydroxyl-1,2,3,4-tetrahydro-isoquinoline hydrobromide(14). Compounds 5-14 were firstly reported. It was found that all the 14 compounds had anti-fungal activity and 6 compounds also showed sperm-killing activities, with compounds 11, 12 having the strongest activities. Conclusion: A group of novel compounds with both anti-fungal and contraceptive activities have been synthesized, which provide a precursor structure for developing new contraceptives with anti-fungal activities.

BACKGROUND:Delayed therapy widely occurs in patients with dementia praecox;therefore,it brings a series of difficulties for clinical treatment and rehabilitation.OBJECTIVE:To analyze the influential factors of delayed therapy in patients with dementia praecox.DESIGN:Cross-sectional study based on patients with dementia praecox.SETTING:The Second Affiliated Hospital of Xinxiang Medical College.PARTICIPANTS:A total of 96 patients with dementia praecox,including 52 males and 44 females,were selected from the Second Affiliated Hospital of Xinxiang Medical College from January to June 2005.Their ages ranged from 16 to 55 years and the mean age was (32.7±12.3) years.Among them,54 patients had middle-school education and 42 patients had high-school education.METHODS:A domestic inventory was used to carefully record basically clinical data of each patient.and the inventory included sex,marriage status,educational level,attack styles,home address,home environment,economic status,family history,etc.All data were classified and analyzed in details.In addition,condition of delayed therapy,which determined as the duration over 1 year from onset of psychiatric symptoms to accepted treatment,in each classification was surveyed gradually.MAIN OUTCOME MEASURES:Delayed therapeutic rate and occurent rate of related factors.RESULTS:Among 96 patients,60 patients had delayed therapy,and the delayed therapeutic rate was 62.5%.Influential factors of delayed therapy:Logistic regression analysis demonstrated that regression equation was involved in educational level,family history,attack style and economic status.Meanwhile,the standard regression coefficient was 0.332 1,0.210 1,0.190 3 and 0.101 2. CONCLUSION:Educationallevel,family history,attack style and economic status of patients with dementia praecox are risk factors of delayed therapy. It is of importance for strengthening these factors to interfere and reduce delayed therapeutic rate at an early phase.

A total of 296 patients were randomly divided into short message group (n =153) and non short message group (n =143).The former group received short message service (SMS) for follow-ups while the latter group had routine oral follow-ups.The return visit rate,recall rate of glycosylated hemoglobin (HbA1c),HbAlc level and glucose control rate for diabetics were assessed after one-year follow-up.The return visit rate (96％) and recall rate of HbAlc (78％) in the short message group were significantly higher than those in the non-short message group (59％,25％,P ＜0.01 for both).The HbAlc level after one-year follow-up (6.51 ± 0.74) ％ was lower than that one year before (6.85 ± 1.26) ％ in the short message group.The glucose control rate after one year (82％) were significantly higher than that one year before in the short message group (65％) and that after one year in the non-short message group(59％,P ＜ 0.01).Use of SMS platform system for follow-ups and health education can effectively improve the return visit rate and glucose control rate,lower the HbA1c level.in diabetics.

Objective To explore the measures to prevent recurrent laryngeal nerve (RLN)injury during thyroid surgery.Methods The clinical data of 223 patients undergone thyroid surgery were retrospectively analyzed.Among the 223 surgeries,69 sides were undergone regional protection act of RLN and 191 sides were performed RLN exposure.Results There were 2 cases of RLN injury from the regional protection operation of RLN,including 1 case of temporary nerve injury which could be resulted from surgery clamp and 1 case of permanent nerve injury which might be caused by mistaking ligation during surgery.There was only 1 case of temporary nerve injury in RLN exposure procedure which was probably caused by the postoperative nerve edema and was recovered 2 months after the operation.The total RLN injury rate was 1.35％.Conclusion For benign thyroid lesions and non-dorsal lesions or during partial excision of the gland,the regional protection of RLN is helpful to prevent RLN injury.In cases with dorsal lesions of thyroid or contralateral RLN injury,or during lobe subtotal resection,lobe resection and reoperation,exposing RLN to prevent injury is necessary.Taking different approaches based on the profiles of lesions and surgical procedures to prevent RLN injury can significantly reduce the risk of RLN injury.

BACKGROUND: Nano-hydroxyapatite (Nano-HA) has been shown to be a good choice of bone repair material owing to its salts composition consistent with natural bone and its scaffold structure homothetic to natural bone structure. OBJECTIVE: To investigate the feasibility of nano-HA in repair of jaw bone defect in rabbits. DESIGN, TIME AND SETTING: Materials-based animal experimental observation was performed at Laboratory Animal Center of Jiamusi University and Beijing Ji Shui Tan Hospital between January 2003 and June 2005. MATERIALS: Neutralization reaction of calcium biphosphate and calcium hydroxide was used to construct the system. The reactants were managed to be cotloidal by reaction control and using appropriate nucleating agents. Acerata HA crystal obtained under different conditions was sintered to obtain the nano-HA granule with a diameter of 1-56 nm. METHODS: Twenty-four healthy Chinese Harbin rabbits were randomly and evenly divided into an experimental group and a control group. After anesthesia, a penetrating 1.5 cm x1.5 cm defect was made with the GX micro-drill at the mandibular edge in each rabbit. Nano-HA was implanted in defects of the experimental group, and common HA was used in the control group. Antibiotics were used for 5 days afterwards. MAIN OUTCOME MEASURES: Change in bone mineral density after implantation of nano-HA. RESULTS: After repair of bone defect, the bone mineral density in the expedmental group increased gradually to a normal level and tended to be stable; whereas it was gradually decreased in the control group. There was significant difference between the experimental and control groups (P < 0.01). CONCLUSION: Nano-HA can promote new bone maturation, and this material produces favorable results in repair of bone defects.

Objective To explore the effect of recombinant pEGFP-N3-APC vectors carrying various APC functional domains on the expression of β-catenin in human colorectal cancer cells HT-29.Methods The recombinant plasmids were transfected into HT-29 cells mediated by lipofectamine~(TM) 2000, and detected by green fluorescence and RT-PCR. Western blot was applied to detect β-catenin expression level in HT-29 cells after transfection, and gray scales of electrophoresis strips were analyzed by SPSS 13.0.Results Green fluorescence and RT-PCR made clear that all 5 recombinant plasmids were successfully expressed in HT-29 cells. Western blot showed that β-catenin expression level in HT-29 cells was not affected after being transfected with pEGFP-N3-APC1, pEGFP-N3-APC2 and pEGFP-N3-APC3, and was distinctly affected after being transfected with pEGFP-N3-APC4 and pEGFP-N3-APC5, especially the later one. Conclusion The selected APC5 gene fragment with 15-amino acid repeats and SAMP repeats, which is relatively short, can degrade β-catenin level in HT-29 cells and may be applied in the gene therapy.