1.Nutritional status of children with chronic disease and clinical nutrition support
International Journal of Pediatrics 2012;(6):627-630
Malnutrition and high nutrition risk exist in children with chronic diseases due to the prolonged course.Malnutrition can not only affect children's growth,but also can increase the risk of nutritionrelated complications,infection,time of hospitalization,hospital costs.Children with chronic diseases have inadequate intake and malabsorption,as well as the increased consumption caused by infection and inflammation.Different diseases need to be given special nutritional support and management,which help improve the clinical outcome and get well for children with chronic diseases.This review summarizes the progresses of nutritional status,nutrition risk assessment and nutritional management of children with chronic diseases.
2.Study on HPLC fingerprint determination Huanglianjiedu Decoction
Chinese Traditional Patent Medicine 1992;0(07):-
Objective:To establish a HPLC fingerprint determination which could separate the main principles of alkaloids, flavonoids and iridoids in Huanglianjiedu Decoction. Methods: The HPLC method was used and chromatography conditions were C 18V column, binary or ternary solvent-delivery gradient elution and diode-array detector. Results: A HPLC fingerprint determination method was established, which could separate main principles of the three effective groups of Huanglianjiedu Decoction. Conclusion: The ingredients of traditional Chinese medicine consist of several effective groups with different chemicals and physical properties, compared with the plant standard extracts, further studies are required for establishing HPLC fingerprint.
3. Quantitative analysis of NPTX2 hypermethylation in pancreatic cancer and its application in diagnosis of pancreatic cancer
Academic Journal of Second Military Medical University 2010;29(11):1279-1283
Objective: To investigate the methylation level of NPTX2 gene in pancreatic cancer and assess its clinical value in diagnosis of pancreatic cancer. Methods: We detected the mRNA expression and methylation levels of NPTX2 gene by qRT-PCR and quantitative methylation with SYBR Green in 10 primary pancreatic carcinoma tissues and the matched adjacent normal tissues. The methylation levels of NPTX2 gene were then detected and compared using the above-mentioned method in the whole blood of patients with pancreatic ductal adenocarcinoma and healthy volunteers. Results: The relative quantification (RQ) of NPTX2 expression in pancreatic carcinoma tissues was significantly lower than that in the adjacent tissues (0.276±0.263 vs 3.526±3.037, P = 0.001). The result of quantitive methylation indicated that the methylation index (MI) of the carcinoma tissues was significantly higher than that of the adjacent tissues ([9.02±7.52]% vs [1.28±0.98]%, P = 0.003). MI of NPTX2 gene was negatively correlated with RQ value (R= -0.552, P = 0.012). We also found that the MI of the whole blood DNA methylation of patients with pancreatic cancer was significantly higher than that of healthy volunteers ([1.80±1.76]% vs [0.84±0.45]%, P < 0.05). Conclusion: Our findings strongly suggest that NPTX2 gene is aberrantly hypermethyated in pancreatic ductal adenocarcinoma. Detection of MI of NPTX2 gene might be of great value for diagnosis of pancreatic cancer.
4. Inhibition of Testudinis Carapax et Plastrum extracts targeting BMP4 pathway on PC12 cell apoptosis
Chinese Traditional and Herbal Drugs 2011;42(1):108-113
Objective: To observe the protection of Testudinis Carapax et Plastrum extracts (TCPE) on serum starvation-induced PC12 cell apoptosis and explore its mechanism. Methods: The PC12 apoptosis model was established by serum starvation for 3 d. The cells were randomly divided into four groups: control group, model group, low-dose and high-dose (3 and 30 μg/mL) TCPE groups. In the three days of the treatment, cell absorbance was determined by MTT, ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), Caspase-3, BMP4, BMPR-IA, and p-Smad1/5/8 signaling molecular expression were detected by Western blotting, and the anti-apoptotic effect of TCPE was observed after blocking BMPs signal pathway. Semi-quantitative analysis of bands was carried out by Bio-Rad Quantity One gel analysis system. Results: MTT and FCM analyses demonstrated that TCPE could increase PC12 cell viability and decrease their apoptotic ratios in a dose dependent manner. Western blotting results showed that TCPE could decrease Caspase-3 expression, promote the expression of BMP4, BMPR-IA, and p-Smad1/5/8. There was statistically significant difference between TCPE (3 and 30 μg/mL) groups and model group (P<0.05, P<0.01) in all above results. While TCPE had no effect on the expression of BMP2, BMP7, and BMPR-II. BMPR-IB hadn't been detected. The anti-apoptotic activity was partially mitigated by neutralizing BMP4 antibody. Conclusion: TCPE has the capacity to inhibit the apoptosis of PC12 induced by serum starvation in a dose dependent manner and its mechanism may be associated with partially activating and up-regulating the expression of BMP4 signaling pathway.
5.Research progress of cyclic peptides derived from phage display technology
Jia-wen CAO ; Dan-yan CAO ; Bing XIONG
Acta Pharmaceutica Sinica 2021;56(5):1253-1264
Cyclic peptide drugs have gradually become an emerging research direction due to their some favorable properties such as high-efficiency binding affinity, high selectivity, lower toxicity, and stable metabolism. In recent years, the number of cyclic peptide drugs under clinical research has continued to increase. Unlike the previous cyclic peptide drugs, which were mostly derived from natural products and their derivatives, these cyclic peptide drugs are designed by genetically encoded display technologies which are based on rational design and
6.Effects of soluble CD14 on the activity of human gingival fibroblasts induced by lipopolysaccharide
Benxiang HOU ; Jun YIN ; Jia CAO
Journal of Practical Stomatology 1995;0(04):-
Objective:To observe the effect of soluble CD14 (sCD14) on the inducing activity of lipopolysaccharide(LPS) on human gingival fibroblasts(HGFs). Methods:In vitro cultured HGFs were stimulated by FITC labeled LPS at the concentration of 0 (control), 100 ng/ml, 100 ng/ml LPS+100 ?g/ml sCD14 and 100 ng/ml LPS+100 ?g/ml ENP respectively for 24 h. The binding activity of LPS to HGFs was measured in the level of mean fluorescence intensity of FITC-LPS.TNF-? and IL-6 in the culture supernatant were measured by ELISA. Results:The binding activity of FITC-LPS to HGFs and the level of TNF-? and IL-6 in culture supernatant were significantly decreased in sCD14 and ENP group(P
7.Bile acid induced gut hormones and hepatic glucose metabolism
Chinese Journal of Diabetes 2015;(12):1134-1137
[Summary] Bile acid is a main component of bile ,which plays a key role in keeping cholesterol metabolism balance in vivo and promoting lipids digestion in intestine. Recently ,more and more researches focus on bile acid for its regulating effect on glucose ,lipid and energy metabolism as a signal molecule. The reabsorbed bile acid stimulates the secretion of fibroblast growth factor 19 (FGF19) and glucagon-like peptide-1(GLP-1) in the intestine by activating a nuclear receptor farnesoid X-activated receptor (FXR) and a membrane receptor TGR5. FGF19 and GLP-1 regulate hepatic glucose metabolism through different pathways. Here ,we briefly summarize the research progress and relationship between bile acid induced gut hormones and hepatic glucose metabolism.
8.Influence of cyclooxygenase inhibitor on expression of nitric oxide synthase in rats with portal hypertension
jia, XU ; hui, CAO ; zhi-yong, WU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
Objective To determine the mechanism of nitric oxide synthase(NOS) and prostacyclin(PGI2) acting on splanchnic hyperdynamic circulation of portal hypertention(PHT). Methods Ninety-six Sprague-Dawley rats were divided into three groups, namely, intrahepatic portal hypertension(IHPH, n=31), prehepatic portal hypertension(PHPH, n=33) and sham-operated controls(SO, n=32). Animals of each group were received indomethacin(INDO) either on a short term or long term with saline as control. Portal venous pressure, together with the concentration of nitric oxide (NO) and PGI2 in serum was measured. The constitutive nitric oxide synthase(cNOS)and inducible nitric oxide synthase(iNOS)activity in the abdominal aorta and small intestine of these rats were detrmined by spectrophotometry method. RT-PCR was performed to measure the levels of iNOS and cNOS mRNA in the arteries and guts mentioned above. Results Although INDO decreased the concentration of PGI2 in serum, the long-term INDO-treated group restored splanchnic hyperdynamic circulation in both IHPH and PHPH rats, concomitant with enhanced expression of iNOS and concentration of NO(P0.05). Conclusion Overproduction of NO inducing hemodynamic abnormalities of PHT is synthesized principally by increase of iNOS. There may be a possible interaction between PGI2 and NO in hyperhemodynamics of PHT, while PGI2 may not be a mediator in the formation and development of hyperdynamic circulatory state.
9.Present status and future prospect of accreditation of proficiency testing provider in the area of laboratory medicine in China
Rujing JIA ; Bingnan ZHAO ; Shi CAO
Chinese Journal of Laboratory Medicine 2016;39(4):326-328
The accreditation of Proficiency Testing Provider ( PTP) has gone through more than a decade in China.Over the past ten years, from nonexistence to existence, the domestic PTPs in the area of laboratory medicine have gradually standardized and developed.By reviewing the international and domestic practice, this paper gives an outline of the present status of the accreditation of PTP in the area of laboratory medicine, explores the difference with other countries, the main problems and some suggestions for improvement, and makes a prospect of the development of the accreditation of PTP in the area of laboratory medicine in China.
10.Determination of Related Substances in Donepezil Hydrochloride Tablets by HPLC
China Pharmacy 2005;0(13):-
OBJECTIVE:To establish an HPLC method for the determination of related substances in Donepezil hydrochloride tablets.METHODS:The separation was performed on AlltimaTM C18 column with mobile phase consisted of 0.01 mol?L-1 dipotassium hydrogen phosphate(3% triethylamine,pH=4.3)-methanol(60 :40) and flow rate of 1.0 mL?min-1.The detection wavelength was set at 271 nm and the column temperature was maintained at 35 ℃.The content of related substance was calculated by self-control with main component.RESULTS:Related substances were completely separated from the main constituent and the impurities were separated well from each other.The determination limit was 0.05 ng and the contents of related substances were 0.42%.CONCLUSION:The method is specific,sensitive and reproducible for the determination of related substances in Donepezil hydrochloride tablets.