Objective To observe the relationship of liver ultrasound class and urinary albumin excretion ratio (UAER) in type 2 diabetes mellitus (T2DM) combined with nonalcoholic fatty liver disease (NAFLD). Methods One hundred and ninety-seven T2DM patients were divided into 3 groups according to the degree of hepar adiposum: group A (66 subjects without NAFLD), group B (63 subjects with mild NAFLD) and group C (68 subjects with moderate or severe NAFLD). Their clinical indexes,UAER and biochemical parameters were measured and compared, the relative analysis of blood fat, HOMA-IR and UAER was done. Results Compared with those in group A, the levels of UAER were significantly increased [(86.49 ± 65.19) mg/24 h vs. (115.16 ± 101.99) mg/24 h vs. (159.45 ± 149.08) mg/24 h,P ＜ 0.05], and the levels of high density lipoprotein cholesterol decreased in group B and group C[(1.21 ± 0.37) mmol/L vs.(1.05 ± 0.38) mmol/L vs. (0.99 ± 0.21) mmol/L,P ＜ 0.05]. Multiple stepwise regression analysis showed that triglyeride was the most important risk factor affecting UAER(P ＜ 0.05). Conclusions There is a close relationship between NAFLD and UAER in T2DM. In the subjects with moderate or severe NAFLD, the UAER increases which indicates that these patients already have capillary vessel injury apparently.

Forty-two patients with type 2 diabetes mellitus (DM) were refered to 3 groups:type 2 DM without diabetic nephropathy (DN),type 2 DM with DN in the initial stage and type 2 DM with DN in the clinical stage.Ten healthy subjects were served as control group.Plasma adrenomedullin (ADM),urinary?_1- microglobulin (MG) and?_2-MG were detected.The results showed that the level of plasma ADM rose gradually with the development of DN and was positively correlated with markers of tubulointerstiial injury such as urinary?_1- MG and?_2-MG (both P

Objective To observe the effects of Atorvastatin on matrix matalloproteiases-2 (MMP-2) level in patients with stable an ginapectoris(SAP) and acute coronary syndrome(ACS).Methods Sixty SAP and sixty ACS patients were randomly divided into either the Atorvastatin group or the control group.The levels of MMP-2 before and after treatment were detected.Results After treatment with Atorvastatin,the levels of MMP-2 in both two groups significantly de- creased(P

1000 IU/L,TBil 40～250 mg/L,and mild liver dysfunction at beginning of the disease,but acute severe hepatic lesion occurred due to the use of antibiotics,immunodepressant or NSAIDs.Three patients developed liver function failure and died,7 patients' liver function recovered to nor- mal by increasing dosage of glucocorticoid.Conclusion AOSD with mild liver dysfunction is frequently en- countered.During the treatment,acute severe hepatic lesion may occur due to certain drugs.Therefore,any drug used in AOSD with mild liver dysfunction should be cautious.Once acute severe hepatic lesion happens, increases the dosage of glucocorticoid promptly can improve prognosis.

Objective: To explore the impact of echocardiographic parameter of diastolic dysfunction (E/A) on the recurrence of atrial ifbrillation (AF) in patients after catheter ablation.
Methods: We retrospectively studied 277 consecutive AF patients with circumferential pulmonary vein ablation (CPVA) in our hospital. According to E/A ratio, the patients were divided into 3 groups: Normal group, the patients with 0.75< E/A<2, n=203, Mild abnormal group, E/A≤0.75, n=53 and Severe abnormal group, E/A>2, n=21. The late AF recurrent rates were compared among different groups. The patients were divided into another 2 groups upon AF recurrence after CPVA: Recurrent group, n=57 patients with atrial arrhythmia lasted more than 30 seconds at 3 months after CPVA and Non-recurrent group, n=220. The clinical conditions were compared between 2 groups.
Results: The patients were followed-up for (374 ± 276) days. The AF recurrent rate in Severe abnormal group was 33.3%, in Mild abnormal group was 23.5% and in Normal group was 18.5%, P>0.05. The E/A ratio in Recurrent group was 1.22 ± 0.54, in Non-recurrent group was 1.19 ± 0.49, P=0.653. Univariate analysis indicated that the risk factors for AF recurrence included LVEDD and gender, not E/A ratio (P=0.236). Cox multivariate analysis indicated that with adjusted gender, hypertension, diabetes, LVEDD and LVESD, abnormal E/A ratio was the predictor for late AF recurrence (HR 2.29, 95%CI 1.01-5.19, P=0.046). With further adjusted LVEDD and E/A, the severe abnormal E/A ratio was still the predictor for AF recurrence (HR 2.27, 95%CI 1.01-5.12, P=0.047).
Conclusion: E/A ratio was the important predictor for AF recurrence in patients after CPVA.

Objective To induce bone marrow mesenchymal stem cells(BMSCs) or bone marrow stroma stem cell(MSCs) to differentiate directionally towards chondrocytes in vitro and then identify the differentiated cells.Methods Bone marrow was harvested from the iliac bone of 16-week-old Japanese white rabbits.After gradient centrifugation,cultivation,amplification,the 3rd-passaged BMSC were implanted in six-hole-plate according to a certain proportion and induced by chondrogenic inducers including transforming growth factor-?1,dexamethasone and vitamin C.Chondrocytes were selected and fixed at different time.The features of chondrocytes were identified by toluidine blue staining and collagen types II immunohistochemical assay.Results The structure of cellular cartilage from BMSCs was uniformly positive of toluidine blue staining and collagen types II immunohistochemical staining.Conclusion Rabbit BMSC,obtained under the experimental conditions,develops stably,proliferates rapidly and is differentiated successfully into chondrocytes by induction in vitro.

Objective To develop a promising type of radiation dosimeter based on doped hydroxyapatite,and to study the stability of dosimetric characteristics indepth.Methods The samples prepared by stereotyping techniques were stored under different temperatures,humidity and illumination conditions after 60Co γ-ray irradiation.Electron spin resonance (ESR) technique was used to quantitatively measure the radiation-induced free radical signal.Results The signal change was less than 3% when the dosimeter was preserved at 4℃ or room temperature within 3 months in the experiment.At 40℃,the signal changed by about 13%,but at room temperature with the humidity less than 36%,the signal changed less than 2%.The change rose to about 8% when humidity was 76%.However,no significant decay of signal strength occurred at relatively high temperatures and under high humidity conditions.When the samples were stored under average illumination of 1600 lux or in a light-resistant container,the signal changes were less than 3.8% or 3.4% respectively.Long-term stability inspection at room temperature suggested a signal change within 4.8%.Conclusion The dosimetric properties of the material don't change significantly below room temperature in a natural environment and exhibit good stability over long-term storage.The free radical signal is not influenced drastically by relatively strong light exposure.However,a high temperature or a highly humid environment may have some effect on the measurement process,which should be taken into consideration in further applications.

Objective To provide anatomical basis for elevating iliac tissue flap pedicled on the iliolumbar artery.Methods The course,number,outer diameter and distribution of iliolumbar artery were observed on 13 human cadavers.Results All the iliolumbar artery originated from the internal iliac artery.The iliolumbar artery gave off 2 branches (iliacus branch and lumbar branch) when passing between the obturator nerve and the lumbosacral trunk,posteriorly to the psoas major.The mean distance between origin of the iliolumbar artery and bifurcation point to iliacus and lumbar branches was 7.1 (7.1 ± 0.5) cm.The iliacus branch divided into two branches:one artery curved forward and anastomosed with the iliacus branch of deep circumflex iliac artery; the other artery supplied the tissue around the posterior superior iliac spine.The lumbar branch supplied the psoas major and the quadratus lumborum.Conclusion The iliolumbar artery and the iliacus branch is one of the most constant and reliable vessels supplying the iliac bone,and can be used as the pedicle of free or pedicled iliac tissue flaps.

Alpha-glucosidase inhibitor can reduce the postprandial hyperglycemia and have good effect on preventing and treating the diabetes and diabetic complication. Along with the application of acarbose which is a kind of alpha-glucosidase inhibitor, many research groups pay attention to the crude alpha-glucosidase inhibitor screened from the medicinal herbs in order to find new, safe, and effective medicine. The development of alpha-glucosidase inhibitor screened from the medicinal herbs and its evaluation in vivo and vitro as well as the varieties of the medicinal herbs that contain alpha-glucosidase inhibitor in recent 30 years were summarized in this paper.
Cell Line ; Drug Evaluation, Preclinical ; Enzyme Inhibitors ; isolation & purification ; pharmacology ; Glycoside Hydrolase Inhibitors ; Humans ; Plant Extracts ; isolation & purification ; pharmacology ; Plants, Medicinal ; chemistry

OBJECTIVETo study the serum protein differential expression in acute leukemia patients and healthy control by differential protein mass spectrometry.

METHODSSerum proteins of 51 acute leukemia (AL) patients and 10 healthy donors were extracted from their peripheral blood. After removing high abundance protein, serum low abundance proteins were separated by two dimensional gel electrophoresis, the differences of serum proteins in AL patients and healthy human were identified. The protein spots with differential expression were cut out and then undergone bleaching, gel digestion and peptide extraction. The peptide mass fingerprint analysis was performed by using MALDI TOF/TOF MS. The protein database MSDB Masort retrieval program was used to evaluate the results.

RESULTSUsing Student's t test,19 statistically significant abnormal expression proteins in the serum of AL patients were found compared with the healthy controls (P < 0.05). The expression of α1-trypsin inhibitor (P < 0.01), prealbumin (P < 0.01), trypsin inhibitor (P < 0.01), apolipoprotein E (P < 0.01) and apolipoprotein A-Ⅳ (P < 0.01) decreased, while retinol binding protein (P < 0.05), globin HP2 (P < 0.05), serum lectin (P < 0.05), H factor homologue protein (P < 0.05) and serum amyloid A1 (P < 0.01) increased. Further stratified analysis found that high serum lectin expression in AL patient resulted in poor outcomes.

CONCLUSIONThere are a variety of serum proteins with differential expression in peripheral blood of AL patients. The differential expression of serum lectin is related to the therapeutic effect. The differential expression of these proteins can be used as a new diagnosis marker or prognostic indicator for acute leukemia.

Acute Disease ; Adolescent ; Adult ; Aged ; Blood Proteins ; metabolism ; Case-Control Studies ; Female ; Humans ; Leukemia ; blood ; diagnosis ; Male ; Middle Aged ; Peptide Mapping ; Proteome ; metabolism ; Young Adult