Congenital fiber-type disproportion (CFTD) is a rare form of congenital myopathy,characterized by non-progressive generalized muscle weakness from early childhood.Diagnosis depends on crucial histological abnormality that type 1 muscle fibers are consistently smaller than type 2 fibers in the absence of other specific histological abnormalities.Whether CFTD should be regarded as a distinct diagnostic entity has always been a controversial issue.Many pathogenic genes have been identified in recent years.This article reviews clinical manifestation,pathology,genetic diagnosis and treatment progress of CFTD.

Anti-angiogenesis therapies have emerged as new treatment strategies for several types of tumors. To date, the most studied programs are to find out effective medicines to block the vascular endothelial growth factor (VEGF) signaling pathway. However, VEGF inhibitors are not effective to all kinds of tumors.Thus there is a need to explore other angiogenesis signaling pathways. Recently, the Dll4-Notch pathway was identified as a new target in tumor angiogenesis. Moreover, an important feature in angiogenesis is the manifold ways in which the VEGF and Dll4-Notch pathways interact each other, which play critical roles in tumor angiogenesis. Therefore, the further exploration of these two pathways will provide more therapies for clinic to treat tumors.

Objective To assess the validity of using fasting plasma glucose （FPG） and HbA1c for the screening of diabetes. Methods 1118 subjects （489 men, 629 women） in Beijing area underwent an oral glucose tolerance test （OGTT） for screening diabetes. HbA1c was examined at the same time. They have never undergone OGTT and controlled plasma glucose levels by any methods. Their average age was 48±12 years. Results Using 1999 WHO criteria, 510 had normal glucose tolerance （NGT）, 35 had impaired fasting glucose（IFG）, 155 had impaired glucose tolerance （IGT）, 52 had IGT and IFG, 366 had diabetes. Using a receiver operating characteristic curve （ROC curve）, the optimal cut-point of FPG related to diabetes diagnosed by OGTT was 6.2mmol/L that was associated with a sensitivity and specificity of 85.0% and 90.4% respectively. Area under the curve was 0.943 （95% CI 0.9270.959）, a positive likelihood ratio （LR） was 8.9, and a negative LR was 0.2. The optimal cut-point of HbA1c related to diabetes diagnosed by OGTT was 6.2%, which was associated with a sensitivity and specificity of 86.6% and 77.5% respectively. Area under the curve was 0.896 （95% CI 0.8760.916）, a positive LR was 3.9, and a negative LR was 0.2. The cut-point of FPG related to IGT diagnosed by OGTT was 5.1 mmol/L, which was associated with a sensitivity and specificity of 65.2% and 68.3% respectively. Area under the curve was 0.729 （95% CI 0.6890.769）, a positive LR was 2.1, and a negative LR was 0.5. The cut-point of HbA1c related to IGT diagnosed by OGTT was 5.7%, which was associated with a sensitivity and specificity of 63.3% and 56.5% respectively. Area under the curve was 0.634 （95% CI 0.5910.677）, a positive LR was 1.5, and a negative LR was 0.7. Conclusions When FPG<7.0 mmol/L and ≥6.2 mmol/L or HbA1c≥6.2%, OGTT was necessary to confirm the diagnosis of diabetes. FPG or HbA1c was not reliable to identify IGT.

As a kind of polyphenolic phytoalexin,resveratrol exerts protective roles in anti-tumor,antiinflammatory and promoting metabolism.Resveratrol's anti-tumor mechanisms may be involved in upregulating tumor suppressor microRNAs and downregulating oncogenic microRNAs,and affect its relative pathways and target genes in different cancers such as colon cancers and breast cancers.This will provide a new theoretical basis for the further clarification of resveratrol-associated tumor suppressing mechanisms and its application to the clinical.

Alogliptin is a novel dipeptide peptidase-4 inhibitor approved for the treatment of type 2 diabetic mellitus.Numerous clinical studies showed that alogliptin alone or in combination with other oral antidiabetic drugs or insulin can substantially control the level of plasma glucose and glycated hemoglobin (HbA1c) in type 2 diabetic patients.12.5 to 25 mg alogliptin alone once daily reduced HbA1c by 0.56％ to 0.59％ ; while combination with other antidiabetic agents resulted additional HbA1c lowering of 0.4％ to 0.8％.Alogliptin was well tolerated,with low incidence of hypoglycemia and no weight gain.Furthermore,alogliptin displayed no extra cardiovascular risk in patients with cardiovascular diseases.

Objective To compare dexmedetomidine versus midazolam sedation for myocardial protection in the patients undergoing percutaneous coronary intervention (PCI).Methods Sixty patients with acute myocardial infarction who required for emergency PCI,were selected and randomly divided into 2 groups (n =30 each) using a random number table:dexmedetomidine group (group D) and midazolam group (group M).In group D,a loading dose of dexmedetomidine 1 μg/kg was infused intravenously for 10 min before surgery.In group M,midazolam 0.05 mg/kg was injected intravenously before surgery.Dexmedetomidine 0.2-1.4 μg· kg-1 · h-1 and midazolam 0.02-0.10 mg·kg-1 ·h-1 were given via a pump during surgery until the end of surgery in the two groups.The RASS score was maintained at-2-2 in two groups.Before administration and on 3rd day after surgery,cardiovascular color doppler ultrasound was performed to record left ventricular ejection fraction (LVEF),venous blood samples were collected for determination of the levels of serum myocardial enzyme and cardiac troponin I (cTnI),and the development of surgical complications,duration of ICU stay and length of hospital stay were recorded.Results Compared with group M,the activities of serum creatine kinase,creatine kinase isoenzyme-MB,aspartate amino transferase and lactic dehydrogenase and cTnI concentrations were significantly decreased,LVEF was increased,and duration of ICU stay and length of hospital stay were shortened in group D.There was no significant difference in the development of postoperative complications between the two groups.Conclusion Dexmedetomidine sedation provides better efficacy for myocardial protection and prognosis and is more suitable for sedation than midazolam in the patients undergoing PCI.

One of the characteristics and advantages of Chinese medicine is holism,which emphasizes harmony of human and nature.It compares the physiological and pathological change of human body with the natural normal or unmoral change by using thinking of analogy by classification,inferring the internal essence according to external synptoms and signs.And it intends to reveal body black-box,and holds physiological and pathological change from macroscopic level,but there are some fuzziness and ambiguity.With the development of morden science on the microscopic fields,some breakthroughs and theoretical systems are established.As the same,Chinese medicine holism must be micro-adjusted and establish its own micro-holism theory,which will be benefit for itself.

TCM is an opening science. With endless absorption of the contemporaneous achievement,organic combination of contemporaneous new theories,and under the guidance of the own philosophical methodology,the TCM achieves innovation and development. However,under the background of modern technology,the theory of TCM has not been innovated far insufficiently,which effects the development of TCM to a great extent.

Objective To construct prokaryotic cell expression vector of human Eotaxin mutants.Methods By point mutation,eight amino acid residues in the N-terminal(residues of 3-7)and N-loop(residue 14)regions of Eotaxin were individually mutated to methionine and residue 14 was delleted or methiomine was inserted after the residue 14,and then cloned respectively into prokaryotic cell expression vector-PET30a+.Results Eight N-terminal and N-loop mutants of human Eotaxin and their prokaryotic cell expression vector-PET30a+ were gained.Conclusion The successful construction of prokaryotic cell expression vector of human Eotaxin mutants lays a foundation for their expression and biological activity and for filtering antagonists of CCR3.

Objective To study hepatotoxicity and superoxide dismutase(SOD) acitvity produced by mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride.Mehtods Hepatotoxicity and superoxide dismutase(SOD)activity of mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride were detected by ip in mice.Results The liver weights were increased consistently during three weeds treatment.It was showed in histology study that hepatocytes swelling,necrosis and congestion wigh blood in venae interlobares hepatis.The SOD activity of liver and blood decreased after one week treatment but increased the following two weeks treatment.Conclusions Mixture of Nickel Sulfate,Potassium Dichromate and Cobalt Chloride had obvious hepatotoxicity and a certain lipid peroxiodation effect in vivo.