Bibliometrics ; China ; Publishing ; statistics & numerical data

Objective To study the effect of vitamin D3 on breast cancer xenografted on a nude mouse model. MethodBalb/c nude mouse was injected subcutaneously with 10 6 human breast cancer cell line MCF-7.Two thousand units Vitamin D3 was administered in mice i?m every the other day. Tamoxifen (5 mg/kg) was fed per mouse daily. Mice were divided into four groups with ten mice in each group: Vitamin D3 group, Vitamin D3 and tamoxifen group, tamoxifen group and control group. After 4 weeks, mice were killed to measure volume of tumor and calcium and phosphorus levels in serum. Tumor cells were analyzed with flow cytometery to detect apoptosis and cell cycle. Results Calcium levels in serum of mice treated with vitamin D3 were higher than that of other two groups, but phosphorus levels in serum were lower . Apoptosis rate was higher than that of control group and tumor cells were arrested in G0/G1 phase, especially those treated with vitamin D3 and tamoxifen. ConclusionsVitamin D3 could significantly induce apoptosis of breast cancer cells and arrest tumor cells in G0/G1 phase. It is synergic with tamoxifen and this may have clinical significance in the treatment of breast cancer.

Animals ; Birds ; virology ; China ; epidemiology ; Humans ; Influenza A virus ; classification ; pathogenicity ; Influenza Vaccines ; Influenza in Birds ; epidemiology ; prevention & control ; Influenza, Human ; epidemiology ; prevention & control

Objective: To investigate the pathologic characteristics and differential diagnosis of pancreatic cancer among residents in Shanghai urban area. Methods: A population-based large case-control study was performed in patients with pancreatic cancer between December 2006 and January 2011 in Shanghai urban area. The pathologic slides from 387 cases of pancreatic cancer were collected and reviewed by five experienced pathologists. The pathologic diagnosis was determined according to WHO Classification of Tumors of the Digestive System, Fourth Edition (2010). Results: Three hundred and fifty cases (90.4%) were confirmed as pancreatic cancer. Most of the pathologic slides were obtained from resected tissues of pancreatic cancer (311/350, 88.9%). The average diameter of the tumor was 4 cm, and 56.6% (n = 198) of the tumors were located in the head of pancreas. The ductal adenocarcinoma was the most common histologic subtype (n = 311, 89.0%), followed by pancreatic neuroendocrine tumors (n = 15, 4.3%). The most common histologic grades were II/III (n = 252). According to TNM staging system, 40 cases had stage IA, 209 cases had stages IB/IIA, and 80 cases had stage IIB. The perineural invasion was identified in 175 cases, and the lymphovascular and/or venous emboli were identified in 56 cases. Only one case was misdiagnosed. Conclusion: The understanding of pathology of pancreatic cancer in Shanghai urban area was improved through this pathologic review. Copyright© 2012 by TUMOR.

Adenocarcinoma, Papillary ; diagnosis ; metabolism ; Carcinoma, Pancreatic Ductal ; diagnosis ; metabolism ; Cystadenoma, Mucinous ; diagnosis ; metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Mucins ; classification ; genetics ; metabolism ; Pancreas ; metabolism ; Pancreatic Neoplasms ; diagnosis ; metabolism

Aim To investigate the effects of propafenone on myocardium inotropism and explore it’s possible mechanism in isolated papillary muscle of guinea pig. Methods Developed tension (DT), maximum rate of contraction (+dT/dt_ max) and maxi-mum rate of relaxation (-dT/dt_ max) were measured during propafenone perfusion before and after administration of L-type calcium channel blocker, nicardipine and selective Na+/Ca 2+ exchanger inhibitor, KB-R7943. Results ①At concentration of 0.1,1,10,30 ?mol?L -1,propafenone attenuated DT from control (0.18?0.05) g to (0.14?0.03), (0.12?0.03), (0.08?0.02), (0.05?0.02) g respectively (P

Air Pollutants ; Cardiovascular Diseases ; epidemiology ; Humans ; Particulate Matter

Active Transport, Cell Nucleus ; Capsid Proteins ; physiology ; Heparan Sulfate Proteoglycans ; physiology ; Humans ; Oncogene Proteins, Viral ; physiology ; Papillomaviridae ; genetics ; physiology ; Papillomavirus Infections ; etiology ; Viral Structural Proteins ; physiology ; Virion ; physiology ; Virus Assembly

Air Pollution ; Atherosclerosis ; etiology ; Humans ; Particulate Matter

Objective To compare the levels of thrombomodulin in acute cerebral infarction patients with different blood glucose levels.Methods Four hundred and eighty-nine patients with acute cerebral infarction were selected including 237 patients of non-diabetes (non-diabetes group),117 patients of diabetes and HbA1c ≤7.0％(diabetes controlled well group) and 135 patients of diabetes and HbA1c ＞7.0％ (diabetes controlled badly group).The level of thrombomodulin was assayed by enzyme-linked immunosorbent assay and compared among three groups.Results The level of thrombomodulin was (2.66 ± 1.20) μg/L in non-diabetes group,(2.80 ± 1.43) μg/L in diabetes controlled well group,(3.22 ±1.60) μ g/L in diabetes controlled badly group.There was the increasing of thrombomodulin among non-diabetes group,diabetes controlled well group and diabetes controlled badly group (P =0.030).There was difference between non-diabetes group and diabetes controlled badly group (P =0.008).Conclusion There is the increasing of thrombomodulin in patients with acute cerebral infarction when their blood glucose rising.