Objective To observe the depositing regions of the cerebral neurons containing A?_ 42 neurons and to study the effects of ginkgo leaf extracts and dioyridamole injection on the memory,the deposition of A?_ 42 in the brain of Alzheimer's disease(AD) model rats induced by aluminium chloride(AlCl_3).Methods The experimental AD model of rats was established by 30% AlCl_3.The rats of treatment group received ginkgo leaf extracts and dioyridamole injection for consecutive six months.Latent period and the intensity of electric shock was evaluated by a passive-avoidence test before and after administration respectively.The morphology and the number of A?_ 42 neurons of the model rats brain by light microscope observation as well as immnohistochemistry.Results (1)After treatment,latent period of the rats prolonged and the intensity of electric shock reduced as compared with AlCl_3 group.(2)The depositing regions of A?_ 42 were found mainly in pyramidal cell layer of CA_1 hippocampus formation and polymorphical cell layer of dentate gyrus,external main stratum of entorhinal region and Ⅲ,Ⅵ,Ⅴ cell layer of cortex(parietal,temporal).The color of A?_ 42 positive substance was brown or brown yellow in the cytoplasm and its dendrites or branches.Significant reducing of A?_ 42 neurons occurred in treatment group(hippocampus P0.05).Conclusion Ginkgo leaf extracts and dioyridamole injection can improve the AD model rats ability of learn and memorizing,which mechanisms are achived by preventing A? from depositing and clearing deposited A?.

Objective To study the effects of Qinglongyi polysaccharide on sialic acid (SA) content, Na+, K+-ATP and Ca2+, Mg2+-ATPase activity, and membrane potential in erythrocytes of S180 mice. Methods Using Kit to determine the SA content and Na+, K+-ATPase, Ca2+, Mg2+-ATPase activity, using flow cytometry to determine the membrane potential of erythrocytes of S180 mice. Results In the treated groups with three doses, it showed Qinglongyi polysaccharide could make the SA content increased (P

OBJECTIVE: To investigate the anti-inflammation effect of curcumin and its derivative in vivo. METHODS: Observations were made on the anti-inflammatory effect of curcumin and its derivatives in xylene-induced mouse ear edema and carrageenan-induced paw edema. The plasma T-AOC, MDA and tissue PGE2 levels were measured. RESULTS: Xylene-induced mouse ear edema and carrageenan-induced paw edema were improved by curcumin and its derivatives in different degrees (P < 0.05, P < 0.01), the plasma MDA levels were lowered, the T-AOC levels were increased, meanwhile PGE2 synthesis in the inflammatory tissues were inhibited (P < 0.05, P < 0.01). CONCLUSION: The anti-inflammatory activity of curcumin and its derivatives in mice may be related to increasing antioxidant capacity and inhibiting the PGE2 synthesis. Copyright 2012 by the Chinese Pharmaceutical Association.

Nucleotide binding oligomerization domain (NOD)-like receptor protein 3, NLRP3) inflammasomes regulate the secretion of caspase-1, interleukin-18 (IL-18), IL-1β, and other cytokines, and participates in aging. In recent years, it has been found that NLRP3 inflammasomes are abnormally activated in aging heart and vessels, and inhibition of NLRP3 inflammasomes can alleviate heart aging and vascular aging. This review summarizes the research of NLRP3 inflammasome in heart and vascular aging, and the related drugs to promote the discovery of the mechanism of NLRP3 inflammasome in heart and vascular aging and the development of related drugs.

Anemia, Aplastic ; genetics ; metabolism ; Bone Marrow Cells ; metabolism ; Case-Control Studies ; Cell Line ; Child ; Child, Preschool ; Female ; Fibroblast Growth Factor 2 ; metabolism ; Gene Expression Profiling ; Humans ; Male ; Mesenchymal Stromal Cells ; metabolism

Objective:To explore the relationship between wearing of functional appliance and systemic metabolism of PGE2 in the blood of growing rats.Methods:54 male Wistar rats aged 4-weeks were randomly divided into 3 groups(n =18):Daytime group,all-day group and control group.Self-guided functional appliance was made for mandible advancement of the rats in the 2 experimental groups.One week after appliance wearing,rats were sacrificed at the corresponding time points and blood in femoral artery was taken. PGE2 levels in the blood samples were measured,data were analysed by cosine equation.Results:Hypothesis was true in the control group(P ＜0.05),and was not true in both 2 experimental groups(P >0.05).Conclusion:PGE2 level in rat blood is circadian rhyth-mic,wearing of functional appliance made may disturb the rhythm.

China ; Diabetes Mellitus ; diagnosis ; metabolism ; Glycated Hemoglobin A ; metabolism ; Humans

Objective To evaluate the role of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signal transduction pathway in lidocaine-induced up-regulation of the expression of surfactant protein-A (SP-A) in rat alveolar epithelial type Ⅱ cells (AEC Ⅱ).Methods Healthy male Sprague-Dawley rats were sacrificed and AEC Ⅱ were isolated,purified and incubated in 24-well culture plates (100μd/hole) with density of 1 × 106/ml.After being incubated for 2 h,the culture medium was replaced with serum-free medium DMEM.The cells were randomly divided into 4 groups (n =48 each):control group (group C),forskolin (adenylate cyclase agonist) group (group F),lidocaine 200 μg/ml group (group L),and PKA inhibitor H89 + L group (group P+ L).Forskolin 10 μmol/ml was added to DMEM in group F.Lidocaine 200 μg/ml was added to DMEM in group L.H89 10μnol/ ml was added to DMEM and AEC Ⅱ were incubated for 10 min,and then lidocaine 200 μg/ml was added in group P + L.At 6,12 and 24 h of incubation (T1-3),cAMP content and PKA activity (using ELISA),and expression of SP-A mRNA (by real-time fluorescent quantitative PCR) and SP-A (by Western blot) were measured.Results Compared with group C,the expression of SP-A mRNA and SP-A was significantly upregulated,and cAMP level and PKA activity were increased at T1-3 in group F and at T2,3 in group L.Compared with group L,the expression of SP-A and SP-A mRNA was down-regulated,PKA activity was decreased,and no significant change was found in cAMP level at T1-3 in group P + L.Conclusion Lidocaine can up-regulate the expression of SP-A in AEC Ⅱ of rats through activating cAMP-PKA signal transduction pathway.

The production conditions of glutathione with shaking flask fermentation by the mutant Saccharomyces J-X25, a methionine-defected strain were studied, and the optimum culture conditions are as follows: initial pH6. 0, temperature 30℃, 100ml in 500ml flask, the inoculum size 10% and agitation rate 220r/min. The emphasis was on the stimulating effect on the cells by dioxogen and the sodium lactate as surfactant. Both of which were added at the logarithmic phase of fermentation, and the GSH production was up to 0. 253g/L , 52% higher than the control without the additions. Compared with the production of GSH initial strain that by the mutant in optimum conditions was increased by 79%.

0.05),but had positive relationship with the clinical stages and lymphatic metastasis of the tumor. Conclusion Livin ? is highly expressed in the tissues and peripheral blood cell from the patients of laryngeal squamous carcinoma,which is closely related to the clinical stages and lymphatic metastasis of the tumor.