Objective To assess the effect of inhalation of 20ppm nitric oxide(NO) on gas exchange and hemodynamics in acute lung injury induced by surfactant depletion in piglet.Methods Sixteen female piglets weighing 25-30 kg were induced with thiopental and pancuronium and maintained with continuous infusion of thiopental,fentanyl and pancuronium. The animals were tracheotomized and mechanically ventilated with oxygen(FiO 2=1.0). Femoral artery was cannulated for arterial blood sampling and blood gas analysis.Pulmonary catheter was inserted through the femoral vein for measurement of CVP, mean pulmonary artery pressure(MPAP), PCWP, cardiac output (CO) and sampling of mixed venous blood. Acute lung injury was induced by lung lavage after the method described by Lachmann until PaO 2 was lower than 100mm Hg(PaO 2/FiO 2

OBJECTIVE To explore the suitable hospital infection control measures in health centers of poverty-striken villages,in order to improve the management of hospital infection,decrease hospital infection rate and protect the health of medical staff and patients.METHODS The status quo of hospital infection in health centers of poverty-striken villages,was investigated in 20 small towns health centers with were randomly divided into two groups:test group(n=15)and control group(n=5).The suitable hospital infection control measures were explored from 5 points.The effect of infection control by before-after controlled study of experimental group and randomized controlled study of control group was anal yzed.RESULTS The rate of hospital infection in test group was decreased from 7.60% to 1.98% and at in control group didn't change,the difference was significant.CONCLUSIONS The managements of establishment of the suitable hospital infection control measures in health centers of poverty-striken villages have been put into practice and gained good result.

Objective To investigate the spreading characteristics of plasmid-mediated resistance and the clones dissemination in multidrug resistant Klebsiella pneumoniae.Methods A total of 53 strains of multidrug resistant Klebsiella pneumoniae were successively collected from the First Affiliated Hospital,Zhejiang University School of Medicine during December 2009 and February 2012.Antimicrobial agents susceptibility was determined by Kirby-Bauer (K-B) method,and pulsed field gel electrophoresis (PFGE) was used to investigate the clonality of clinical isolates.Filter mating method was used for plasmid conjugation,and the relative molecular mass of the plasmid was determined using S1-PFGE assay.PCR amplification and sequencing were used to screen common antimicrobial resistance genes.Results Fiftythree clinical isolated Klebsiella pneumoniae strains were all resistant to penicillins,and their sensitive rates to piperacillin/tazobactam,cefoperazone/sulbactam were below 15％.More than 86％ strains were resistant to cephalosporins,and 60％-70％ strains were resistant to quinolones,sulfonamides and tetracyclines.About 50％ strains were resistant or intermediate sensitive to carbapenems.PFGE results did not support the evidence of clone dissemination.Resistant isolates harbored conjugant plasmid with 34 000-355 000 bp size,which encoded penicillins,cephalosporins,carbapenems or aminoglycoside resistant determinants,including CTX-M,TEM,KPC type β-1actamase genes and plasmid-mediated quinolone resistance gene qnr.Conclusions Multidrug resistance is prevalent in clinical Klebsiella pneumoniae isolates.Rapid prevalence of resistance is mainly determined by conjugant plasmid horizontal transfer rather than simple clone dissemination.

We have studied the inhibitory effect of polysaccharides mixture on Ehrlich Ascites Carcinoma (EAC), Sarcoma 180 (S180), reticulocytic leukemia (L615) in mice. It is found that polysaccharides mixture could obviously prolong the survival duration of mice suffering from tumour and inhibit neoplastic proliferation. Thymus and adrenal glands atrophy in mice caused by vaccination of cancer cells was also remarkably resisted.The results of our experiments show that polysaccharides mixture is a safe,effective and promising pharmaceutic preparation of traditional Chinese medicine. It deserves to be further studied and developed.

This paper reports the effects of the polysaccharide from Sargassum fusiforme (SFPS) on contents of lipoid peroxide (LPO) and enzyme activities of glutathione reductase (GR), glutathione peroxidase (GSH - PX), catalase (CAT), superoxide dismutase (SOD) in whole blood, liver and spleen in leukemia L615 mice. The results show that SFPS remarkably reduces the contents of LPO and increases in the enzyme activities of CAT , SOD in whole blood, liver and spleen in leukemia L615 mice. The authors point out that SFPS may remove the free radicals in the body of leukemia L616 mice and have an effect against lipid peroxidation. This may be one of the mechanisms of SFPS against leukemia.

Acetaminophen(AAP)-induced hepatic injury is one of the common causes of drug-induced hepatic injury.Up to date,the mechanisms of AAP-induced hepatic injury are still incompletely understood.Recent advances suggest that reactive metabolite formation,glutathione depletion,alkylation of proteins,especially mitochondrial proteins and peroxynitrite formation are critical initiating events for the toxicity.This review will focus on more recent advances in mitochondrial dysfunction after AAP overdose.Additional,oxidative stress and inflammatory mediators are also important for the overall outcome.

Objective To explore the therapeutic strategy for aged patients (older than 70) with chest tumor. Methods To summarize and analyze the clinical data of 216 aged patients with chest tumor. Results 216 aged patients with chest tumor were involved our study,Their clinical data were summarized and analized,All patients went smoothly through perioperative period,They recovered rapidly and discharged successfully from hospital,With no death occurred,postoperative complications occurred in a total of 36 cases(16.7%), But they were cured through meticulous management. Conclusions Aged patients with chest tumor could obstain good results by strict indications,fully risk evaluation and meticulous perioperative management.

Objective To improve X-ray and clinical knowledge of asphyxiating thoracic dysplasia(ATD).Methods X-ray film and CT findings of twin infants were studied meanwhile relative literature about ATD was reviewed.Results The main positive fingings of ATD were as follow:(1)The thoracic cages were both shorter than usual in the transverse and anterioposterior diameter;the anterior ends of the ribs are widened and cupped;(2)The pelvis also show abnormalities which include some flaring of the ilia,and early ossification of the centers for the capital femoral epiphyses;(3)Incureable respiratory infection was the prominent clinical manifestation.Conclusion ATD,a rarely seen disease,often occurs in those patients who suffer from pneumonia repeatedly;Because plain films can clearly show the relative characteristic deformity changes of thoracic cage and pelvis,X-ray examination can be regarded as the most important diagnostic method.

Purpose: To evaluate the efficacy, safety and survival of low molecular weight heparin ( LMWH) plus chemotherapy in patients with advanced non-small cell lung cancer. Methods: 46 patients with NSCLC were randomized into chemotherapy plus LMWH. (study group) and chemotherapy only( control group). Both groups received two cycles of MVP regimen (MMC 6 mg/m2, YDS 3 mg/m2 x 2, DDP 90 mg/m2). Patients in the study group were treated with LMWH 5000u twice daily from the third day before chemtherapy up to 7 days. Results: The response rate was 56. 5% (13/23) for the study group and 39. l%(9/23) for the control group. Median survival time( MST) and 1-year survival rate were 12, 1 months(95%CI:8.52~14.64) and 52.2% for the study group compare 8.4 months(95%CI:6.15 ~ 10. 85) and 34. 8% for the control group. There were significant differences for MST( 12. 1 vs 8. 4) and 1 year survival rate(52. 2% vs 34. 8%) in the study group as compared to the control group. No difference in response rate and toxicities were found between the two groups. Conclusions: Chemotherapy( MVP regimen) plus LMWH is effective and safe. Prolonged survival was observed in patients who received MVP regimen plus LMWH.