Hepatobiliary iminodiacetic acid (HIDA) scintigraphy is a non-invasive, functional imaging of the hepatobiliary system that serves as an adjunct imaging modality for neonatal cholestasis work-up. In view of the urgency to diagnose
biliary atresia and restore bile flow through surgery, HIDA scintigraphy could help to distinguish between neonatal
cholestasis due to biliary atresia and neonatal hepatitis of various causes. We describe a full-term male infant with
jaundice beyond the physiological period in which HIDA scintigraphy showed absent tracer excretion from the
biliary system into the intestines up to 5 hours on follow-up imaging. The intraoperative diagnosis confirmed the
diagnosis of biliary atresia. The prognosis of the patient with biliary atresia depends on early surgical planning and
intervention. Therefore, non-invasive diagnostic tools play an important role in the evaluation of a child with neonatal cholestasis.