1.Identification of local clinical Candida isolates using CHROMagar CandidaTM as a primary identification method for various Candida species
Madhavan, P.* ; Jamal, F. ; Chong, P.P. ; Ng, K.P.
Tropical Biomedicine 2011;28(2):269-274
The objective of our study was to study the effectiveness of CHROMagar CandidaTM
as the primary identification method for various clinical Candida isolates, other than the
three suggested species by the manufacturer. We studied 34 clinical isolates which were
isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first
cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35ºC, then on CHROMagar plates
at 30ºC, 35ºC and 37ºC. The sensitivity of this agar to identify Candida albicans, Candida
dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and
Candida parapsilosis ranged between 25 and 100% at 30ºC, 14% and 100% at 35ºC, 56% and
100% at 37ºC. The specificity of this agar was 100% at 30ºC, between 97% and 100% at 35ºC,
92% and 100% at 37ºC. The efficiency of this agar ranged between 88 and 100% at 30ºC, 83%
and 100% at 35ºC, 88% and 100% at 37ºC. Each species also gave rise to a variety of colony
colours ranging from pink to green to blue of different colony characteristics. Therefore, the
chromogenic agar was found to be useful in our study for identifying clinical Candida isolates
2.Hyaluronatelyase production by Streptococcus pneumoniae isolated from patients and carriers
Yusof, H.A., Desa, M.N.M., Masri, S.N., Malina, O. and Jamal, F.
Tropical Biomedicine 2015;32(3):413-418
Hyaluronatelyase produced by various microorganisms are capable of degrading
hyaluronic acid in connective tissues and initiating the spread of infection by opening an
access for the pathogen into host tissues. The present study attempts to determine the
distribution of hyaluronatelyase-producing Streptococcus pneumoniae among invasive, noninvasive
and carriage isolates, and correlate it with the clinical sources, year of isolation,
colonial morphology and their serotypes. A total of 100 isolates from various clinical samples
were selected and screened for hyaluronatelyase production and presence of the encoding
SpnHyl gene. All isolates possessed SpnHyl gene. Ninety-six isolates including 34 carriage
isolates were positive for production of hyaluronatelyase. Four hyaluronatelyase-negative
isolates were from blood (2 isolates) and sputum (2 isolates). No significant association was
detected among hyaluronatelyase production and bacterial characteristics except for colonial
morphology (p = 0.040). High percentages of hyaluronatelyase production in these isolates
suggest their possible role as human pathogens.
3.Operative safety and oncologic outcomes in rectal cancer based on the level of inferior mesenteric artery ligation: a stratified analysis of a large Korean cohort
Mohammed A ALSUHAIMI ; Seung Yoon YANG ; Jae Hyun KANG ; Jamal F ALSABILAH ; Hyuk HUR ; Nam Kyu KIM
Annals of Surgical Treatment and Research 2019;97(5):254-260
PURPOSE: To compare high and low inferior mesenteric artery (IMA) ligation in a large number of patients, and investigate the short-term and long-term outcomes. METHODS: This retrospective study compared outcomes between high IMA ligation and low IMA ligation with dissection of lymph nodes (LNs) around the IMA origin. A total of 1,213 patients underwent elective low anterior resection with double-stapling anastomosis for stage I–III rectal cancer located ≥6 cm from the anal verge (835 patients underwent IMA ligation at the IMA origin; 378 patients underwent IMA ligation directly distal to the root of the left colic artery along with dissection of LNs around the IMA origin). RESULTS: There was no difference in anastomotic leakage rate between groups. The 2 groups did not significantly differ in intraoperative blood loss, perioperative complications, total number of harvested LNs, and metastatic IMA LNs. However, more metastatic LNs were harvested in the high-tie than in the low-tie group (1.3 ± 2.9 vs. 0.8 ± 1.9, P = 0.002), and the incidence of positive pathologic nodal status was higher in the high-tie group (37.9% vs. 28.6%, P = 0.001). The 5-year local recurrence-free and metastasis-free survival rates were similar between groups, as were the 5-year overall and cancer-specific survival rates. CONCLUSION: Low IMA ligation with dissection of LNs around the IMA origin showed no differences in anastomotic leakage rate compared with high IMA ligation, without affecting oncologic outcomes. High IMA ligation did not seem to increase the number of total harvested LNs, whereas the ratio of metastatic apical LNs were similar between groups.
Anastomotic Leak
;
Arteries
;
Cohort Studies
;
Colic
;
Humans
;
Incidence
;
Ligation
;
Lymph Nodes
;
Mesenteric Artery, Inferior
;
Rectal Neoplasms
;
Retrospective Studies
;
Survival Rate
;
Treatment Outcome