BACKGROUND: Glycosylation process helps in stabilization and solubilization natural of compounds. Glycosyltransferase (YjiC) provides for high efficient glycosylation product with an incredible variety of sugar moieties, typically from UDP-glucose. Vitamin C (L-ascorbic acid) is an essential nutrient for humans and certain other animal species. Vitamin C functions in many biological processes, such as collagen synthesis, antoxidation, intestinal absorption of iron. UDP-glucose acts as a starting material for glycosyltransferase (YjiC). In order to recycle UDP-glucose after glycosylation with glycosyltransferase (YjiC), sucrose synthase (AtSUS1) carry out than reversible conversion of sucrose and UDP to UDP-glucose and fructose. MATERIALS AND METHODS: DNA was extracted than E.coli BL 21 and E.coli JM 109 hosts were used for expression of proteins. The purified protein was then analyzed by 12% SDS-PAGE than used for enzymatic recycle system. TLC analyse of the products were carried out to the test glycosylation. RESULTS: In this study, we choose substrate vitamin C for the enhancement of enzymatic recycling system glycosylation. In this recycle system due to the high concentration of sucrose and vitamin C but low concentration of UDP-glucose with is relatively expensive made the system more economic. TLC analyses of the products were carried out to the recycled system worked and glycosylation product. CONCLUSION: Based on proved function an enzymatic recycling system with glycosyltransferse (YjiC) and sucrose synthesis (AtSUS1) to be applicable to enzymatic production of vitamin C glucoside and resveratrol glucoside. Further analysis by HPLC and MS will elucidate the products.

BACKGROUND: Tinea capitis is a contagious fungal infection that occurs predominantly in childhood. Although its incidence has declined, its epidemiological and mycological characteristics are continue to evolve. OBJECTIVE: To assess changes in the epidemiological and mycological characteristics of tinea capitis in teenage patients in Southeastern Korea. METHODS: We retrospectively investigated epidemiological and mycological characteristics of 202 teenage patients with tinea capitis who visited Kyungpook National University Hospital and the Catholic Skin Disease Clinic from 1989–2018. RESULTS: Of 202 patients, 177 patients showed KOH-positivity. Dermatophytes were cultured from 157 patients. The annual incidence of tinea capitis ranged from 0 to 30 between 1989 and 2018. Of 202 patients, 153 patients (75.74%) were male and 49 patients (24.26%) were female. The ratio of male to female was 1:0.32. For seasonal distribution, 78 patients (38.61%) visited our hospital in winter, 59 patients (29.21%) visited in spring, 35 patients (17.33%) visited in fall, and 30 patients (14.85%) visited in summer. Microsporum canis was the most common dermatophyte (44.06%) isolated from tinea capitis. Trichophyton tonsurans was the second most common dermatophyte (26.73%). For inhabitancy distribution, 151 patients (74.75%) lived in urban areas and 51 (25.25%) lived in rural areas. CONCLUSION The epidemiological characteristics of teenage patients with tinea capitis were distinct from those of adults in annual incidence, sexual distribution and isolated dermatophytes. Trichophyton tonsurans was the main causative organism of tinea capitis in recent 10 years. These results are useful for the targeted treatment and prevention of tinea capitis.