Objective Biochemistry course,a professional basic course of medical students,is a challenging course,for which the traditional classroom teaching mode is not suitable,for there are many deficiencies in traditional classroom teaching,so new teaching methods need to be explored to improve the quality of teaching and learning efficiency.Method 301 students from 6 natural classes of adult medical education were selected as the object of study and were divided into the experimental group(150) and control group(151).The traditional teaching mode was adopted in the control group,while in the experimental group,the double-track teaching methods were adopted such as diagram method,the method of using body language,humor teaching method,to stimulate students' interest in scientific research and innovation.At the end of the course,students were asked to conduct online teaching evaluation while teachers were asked to conduct mutual evaluation.Examination paper analysis software was used to evaluate the examination paper.Test results were analyzed by using SPSS 15 Statistical Software for data processing.Results:mean ± standard deviation (~ ± s) ; comparison was made between groups in t test.The difference was statistically significant(P＜0.05).Result The evaluation results of students and colleagues assessment show that 93.7％ of the students and 90.9％ of colleagues think that the experimental group uses the double-track teaching method is more popular than in the control group.At the end of the experiment,the control group grades is (66 ± 25),while the experimental group grades is (83 ± 22) ; t value is 6.2612; P value is 0.000.Conclusion Compared with traditional teaching method double-track teaching method has obvious advantages in teaching.From the result,double-track teaching enables students willing to think and have an active participation and exploration,which has significantly stimulated their learning interest and improved their performance,thus achieving good results.

Objective The study was aiming at elucidating the rale of BSP over-expression in enhancing osseous metastasis of breast cancer cells,in order to search for new drug targets for prevention and treatment of such metastasis.Methods Chicken embryo allantois membrane(CAM)was used in the experiment.Breast cancer cells were inoculated on the superior surface of CAM sac,then the vascular tissues were collected in lower sac after incubation.The human specific Alu gene was amplified by PCR with genomic DNA extracted as template.Breast cancer cells were incubated with bone,which had becn treated with collagenase and trypsinase,and then OD_(488) of breast cancer was assessed to evaluate the adhesive affinity of breast cancer cells to bone.Results The findings of CAM assay and PCR amplifica- tion using human Alu primers and genomic DNA showed that Alu gene was amplified from 4 of 5 in MDA-MB-231BO(pI)group and 3 of 5 in MDA-MB-231BO(pID)group,but not amplified in control group.In bone adhesion assay,the OD_(488) of digested bone after MDA- MB-231BO(pI)and MDA-MB-231BO(pID)adhering to bone were 0.58?0.046 and 0.49?0.039,respectively,which were larger than that of MDA-MB-231BO(0.26?0.021,P

Objective To study on the neural mechanism of deficits in Chinese developmental dyslexia from the aspects of the phonological processing,orthographic skills,visual magnocellular function and cerebellum function.Methods Critical words in Chinese and English (e.g.dyslexia,reading development,Chinese,neural) and formula (e.g.Chinese and (reading development) and (neural or neuroimage or fMRI or ERP or brain area) related with the present topic were searched among the article abstracts in Chinese and foreign databases (e.g.CNKI,Pubmed,Sciencedirect) from July to December,2014.Results Fifty-two relevant articles were gained access to the database.Referring to the present topic,research on the neural mechanism of dyslexia with neuroimaging technique was reserved,while the studies in which the reading impairment of the participants was caused by acquired factors were eliminated.Finally,thirty-three valid articles were retained.Conclusion According to previous studies,although there might be similarities in cognitive deficits of dyslexia between alphabetic languages and Chinese,it was still found that the Chinese children with developmental dyslexia exhibited abnormal neural activities and impaired brain structures in areas associated with Chinese phonology (i.e.left middle frontal gyrus,which was different from the left inferior fiontal gyrus always related with phonological processing in alphabetic languages) and orthographic skills (right occipitotemporal areas which was responsible for the visuospatial processing),revealing language specificity of Chinese to some extent.However,some other studies reported the similarities in neural mechanisms of dyslexia across languages.Therefore,more studies were required to further examine the crosscultural mechanism of the neural activity regarding the developmental dyslexia.Meanwhile,researches on the aspects of general perception showed Chinese dyslexic individuals had deficits in visual magnocellular function,and cerebellum.Future studies were required to explore the relationship between the linguistic and non-linguistic deficits.

Objective To investigate the relations between BNP and cTnI leveh in patients with CHF and cardiac function.Methods 75 patients with CHF were selected as observation group and another 30 healthy people were selected as control group.BNP and cTnI levels were detected by electrochemiluminescence immunoassay and the data were analyzed.Results BNP and cTnI of observation group were significantly higher than control group,the difference was statistically significant(t=3.897,4.127,all P＜0.05);comparison of BNP and cTnI levels in different classification of cardiac function of observation group had significant difference(F=4.765,5.876,allP＜0.05);Linear corrlation analysis showed that:BNP and cTnI both positively correlated to classification of cardiac function(r=0.853,0.647,all P＜0.05),BNP and cTnI increased significantly with the increase of cardiac function classification.Conclusion BNP and cTnI increased significantly in patients with CHF and closely related with cardiac function.they were ideal index to evaluate the CHF severity and had a good clinical value to judge the status and function of heart in patients with CHF.

Objective:To observe the efficacy of warm needling in canicular days in treating knee osteoarthritis (KOA).
Methods:Sixty-eight KOA patients were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by warm needling, while the control group was by conventional acupuncture. The intervention started from the first day of the canicular days, once every day, 10 sessions as a treatment course. The improvement and therapeutic efficacy were evaluated after 3 courses of treatment.
Results:The total effective rate was 91.2%in the treatment group versus 67.6%in the control group, and the difference was statistically significant (P<0.05).
Conclusion:Warm needling in canicular days can produce a better efficacy in treating KOA than conventional acupuncture.

In order to train the medical professionals with good English communication skill,this article aims to offer some effective measures to perform bilingual education of clinical hematologic laboratory science smoothly by analyzing the true significance of clinical hematologic laboratory science,researching the effective bilingual teaching,promoting the building of the rank of teachers and adopting various means in the classes.

In recent years the pressure on the employment of college graduates has been increasing,therefore it is necessary to get some knowledge of employment situation.In order to know the employment situation of the medical college graduates,we made a survey on our 2008 batch medical graduates of China Three Gorges University,and also made further analysis and re-flections according to the survey results.We hope our reflections can provide some supports and assistances to the medical graduates in certain aspects.

Objective To study the solute clearance effect of the new concentrated anticoagulation hemodiaiysate of citrate for hemodialysis in patients with high risk of bleeding. Methods Forty-two kidney failure patients with high risk of bleeding were divided into two groups (Group A and Group B) according to their hemodialysis manners. Patients in Group A were hemodialyzed with bicarbonate hemodialysate with low-molecular-weight heparin (dalteparin) anticoognlation and those in Group B with the new citrate antieoagnlation hemodialysate prepared in our hospital without any other anticoagulant. Blood urea nitrogen (BUN) and creatinine (Cr) concentrations were measured before and after dialysis, and Kt/V and urea reduction rate (URR) were calculated. In addition, activated clotting time (ACT) and ionized calcium (iCa2+) concentration were also measured at the arterial and venous ends. Results ACT was extended and iCa2+ concentration decreased significantly at the venous end compared with those at the arterial end in Group B (P<0.01). BUN and Cr concentrations were markedly decreased after dialysis compared with those before dialysis in both groups (P<0.01), and no significant difference in solute clearance effect, as indicated by Kt/V and URR, was observed between Group A and Group B (P>0.05). Conclusion The solute clearance effect of the new concentrated anticoagnlation hemodialysate of citrate is excellent during hemodiaiysis in kidney failure patients with high risk of bleeding.

Objective To investigate the awareness and trainning needs of health management knowledge in primary healthcare professionals in Beijing.Methods Six hundred primary healthcare staff from Daxing,Fangshan,Miyun,Pinggu and Huairou District of Beijing were selected in 2012 through muhiphase random sampling method and required to complete a standardized questionnaire.The survey included the average awareness rates of health management(tobacco,body weight and alcohol consumption control and daily physical exercises),the rates of application and conception awareness of risk assessment tools of chronic diseases,the awareness rates of normal levels of blood pressure and serum glucose as well as training needs.Differences in different regions were analyzed.Chi-square test was used for data analysis.Results The average awareness rates of tobacco,body weight and alcohol consumption control and daily physical exercises were 94.1％ (513/545),95.3％ (522/548),94.0％ (515/548) and 85.9％ (470/547),respectively.However,the rates of application and conception awareness of risk assessment tools of chronic diseases were only 55.1％ (295/535) and 59.8％ (329/550),respectively.Those who knew the normal levels of blood pressure and serum glucose accounted for 85.4％ (475/556) and 92.4％ (514/556),respectively; although only 23.6％ (131/556) and 21.2％ (118/556) were completely familiar with the major risks of these conditions.About 57.4％ (308/537) or 56.7％ (305/538) participants reported urgent needs for health education on diabetes and hypertension management.Conclusion The awareness of health management of primary healthcare professionals in rural areas of Beijing remains lower and may need to be raised by education or training.

Objective To accurately understand multi-spot medical practice in China and evaluate its effect.Methods Operation data of 2008 and 2009 were gathered from all medical institutions in Kunming to analyze the effect of the practice trial using the fixed effect model.Results Under control of such factors as the scale and type of the hospitals,clinic visits of the second certified hospital benefited by the practice increased by 14％than before,whereas the medical revenue of the institution increased by 29％.The effect proves more significant in terms of total clinic visits and medical revenues at level-1 and level-2 medical institutions.Conclusion Multi-spot medical practice in Kunming proves successful as it increased medical service supply and medical revenue,especially for level-1 and level-2 medical institutions.This effect possibly results from releasing high-level physicians down to lower-level institutions,helping these institutions to attract patients.