Objective:To discuss the role of Fas/FasL-mediated colonic epithelial apoptosis in the pathogenesis of ulcerative colitis(UC).Methods:Expression of Fas,FasL and M30(a new epithelium apoptosis marker) antigen on colonic mucosa of 35 patients with UC and 15 control populations were detected by IHC.Each marker was analyzed by image analysis system.Co-expression of Fas and M30 was observed through serial sections and double staining IHC.Results:The expression of Fas,FasL as well as M30 in UC increased considerably compared with controls (P

Adult ; Aged ; Aged, 80 and over ; China ; epidemiology ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Pneumoconiosis ; epidemiology

Neovascularization is one of the crucial challenges in tissue-engineered grafts.In order to find sdution to the problem,endothelial cells and animals were exposed to electromagnetic fields to examine the formation of new vascular.This review gives an overview of the effects of electromagnetic fields on the neovascularization of tissue-engineered grafts.

Objective To explore influence of antibiotics and Jiuyi Dan combined with incision-thread-drawing procedure on perianal abscess patients' level of activin A (ACTA) and C-reactive protein (CRP).Methods120 cases of perianal abscess patients in our hospital during March 2015 to December 2016 Coloproctology were selected, they were divided into the experiment group and the control group by random number method.All of patients were taken incision-thread-drawing procedure.Then the experimental group were given nine one antibiotics and Jiuyi Dan, the control group were given antibiotics.The cure rate, 6months after the recurrence rate, average healing time of the wound, and wound edema rate of two groups were observed.Levels of ACTA and CRP expression levels of two groups of patients before and after operation were analyzed.ResultsPatients in the experimental group cure rate was 96.7% higher than that of the control group (P<0.05), the recurrence rate of patients in the experimental group than in the control group, the difference was not statistically significant;the patients in the experimental group the average healing time was (20.67±3.22) d shorter than the average healing time of patients in the control group (29.48±3.15) d, patients in the experimental group wound edema rate of 5% was significantly lower than in the control group 20% (P<0.05);3D ACTA, the expression level of CRP was significantly higher than the preoperative 7 d levels after operation in the two groups (P<0.05);after 7d, the expression level of the two groups of patients with ACTA and CRP were significantly lower than the preoperative level of expression of 7d (P<0.05), experimental group levels of ACTA were significantly lower than the control group, the difference was statistically significant (P<0.05);postoperative 14d patients, ACTA levels of two groups were significantly lower than the levels before operation (P<0.05).ConclusionPerianal abscess can be treated effectively and patients levels of Acta and CRP were obviously dereased by Jiuyi Dan combined with incision-thread-drawing procedure combined, postoperative inflammatory reaction could be importantly alleviated, it was worth clinical promotion.

OBJECTIVE: To study the effect of samples having different pH values in the bacterial endotoxins test (BET). METHODS: The BET was conducted as an assay of the concentration of endotoxins by using kinetic-turbidimetric technique. Samples having different pH values but containing same concentration reference standard endotoxin (RSE) were tested by TAL/LAL reagents. The average result on the standard curve was calculated and the mean recovery observed. RESULTS: The reagents had three highly sensitivities in pH 5.20, pH 7.83/pH 6.23 and pH 10.55. After adjustment to pH 6 to 8, the mean recovery was 76.5%～115.9% or 85.8%～99.8%. When the sample pH value was less than 3 or more than 12, the test was unsuitable for the inhibition test, and the mean recovery was less than 56.8%. CONCLUSION: It is necessary to adjust the pH of the solution to be tested in the BET, adjust the sample to pH (6.5～7.5) when using TAL reagents, and adjust to pH (6.2±0.1) or pH(6.7～7.8) when using LAL reagents.

Delayed cerebral vasospasm is the leading cause of death in patients with subarachnoid hemorrhage. A good in vivo animal model of cerebral vasospasm after subarachnoid hemorrhage helps to understand the occurrence, development of this cerebral vasospasm, and the complicated and various pathophysiological mechanisms. It will provide important information for clinical interventions

Objective To observe and compare anatomical position between pediatric internal jugular vein and common carotid artery in children patients of different ages with ultrasonic technique.Methods Four hundred and ninety-six cases of pediatric patients undergoing selective operation,ASA physical status Ⅰ or Ⅱ,were selected in this study,they were 380 males and 116 females ranging from 0 to 12 years.Image position of internal jugular vein and common carotid artery above the clavicle were detected with ultrasonic technique for all pediatric patients,positions of internal jugular vein and common carotid artery for different patients were classified according to different angles,anatomical position between pediatric internal jugular vein and common carotid artery in different age groups (newborn group,infant group,toddler group,preschool children group and school-age children group) were compared.Results In area between 0°-30°,the ratios of right puncture of infant group,toddler group,preschool children group and school-age children group were significantly higher than those of the left (infant group: 42.3% vs 0%;toddler group: 48.8% vs 1.6%;preschool children group: 57.5% vs 2.7%;school-age children group: 58.0% vs 2.1%)(P<0.01).In area between 30°-60°,the ratios of the right puncture in all groups were significantly higher than those of the left (newborn group: 45.0% vs 10%;infant group: 44.2% vs 23.1%;toddler group: 46.3% vs 19.5%;preschool children group: 35.4% vs 19.5%;school-age children group: 35.1% vs 20.2%) (P<0.05 or P<0.01).In areas between 60°-90°and at 90°,the ratios of the right puncture in infant group,young children group,preschool children group and school-age children group were significantly higher than those of the left (P<0.01).In areas of >90°,there was no statistical difference between the ratios of the right puncture and the left in all groups.Conclusion The success rate of right internal jugular vein puncture of children was higher than that of the left.Conditions in the newborn group were different from that in the other age groups.

The development of renal transplantation from living relative donors relives the shortage of transplanted organs,and benefits patients with end-stage renal diseases.However,when seen from the aspect of donors,implementing a non-therapeutic invasive surgical treatment on a healthy person still violates the absolute sound principle of medical ethics.To reduce the harm to the minimum,a comprehensive assessment should be conducted before the transplantation,and long-term follow-up observations after transplantation should also be strengthened.

Studying of atherosclerosis is more than a hundred year.There are many theories about this disease,but now most of us seems to agree with the stress-reponse theory.Heat shock proteins are the products produced at the first stage of atherosclerosis.And someone think that it may have something to do with atherosclerosis.This paper discussed the heat shock proteins and atherosclerosis respectively,and emphasized the relationship between them.