100u. It is recommended that the therapeutic principles should be clearing the liver, cooling blood, eliminating poisons hitting the aYing - blood level. To prevent relapse, emphasis should be laid on supporting the body to expei poison, activating the stagnant blood and harmonizing the collaterals.

OBJECTIVE:To establish a simple method for drug bioequivalence test based on Excel function.METHODS:The statistical tests including ANOVA,two one-side test and 90% confidence interval were carried out using Excel statistical function.RESULTS:Drug bioequivalence test can be performed based on Excel,with results in line with those stated in Biopharmaceutical and Pharmacokinetic.Moreover,due to Excel's repeated calculation function,for retest,three kinds of test results were available rapidly only by replacing the data with others remained for calculation.CONCLUSION:Excel function can be used to calculate drug bioequivalence.

Hepatoblastoma (HB) is the most common malignant liver tumors among children. It typically begins with few symptoms, making it dififcult to diagnose and treat. The number of HB cases has been on the rise in recent years, resulting in gradual development of the research on various aspects of the disease. This paper is a round-up of the etiology, pathological classiifcation, clinical staging, prognostic factors and therapeutic principles of hepatoblastoma.

AIM:Anti-hepatis B immunoglobulin in combination with lamivudine is efficient to prevent chronic hepatitis B virus (HBV) reinfection following liver transplantation. However, long-term usage of lamivudine can result in YMDD variation and lead to medicine resistance even HBV relapse. In this study, we investigated etiological factors and prevention and treatment protocol of HBV recurrence and YMDD variation after liver transplantation. METHODS:A computer-based online search of Pubmed database from January 2002 to January 2008 and Chinese Journal Full-text Database from January 2003 and December 2007 was undertaken to identify articles about HBV recurrence and YMDD variation after liver transplantation. The collected articles were firstly selected and the references of each article were looked up. Only articles that involved in HBV recurrence and YMDD variation after liver transplantation were included. The articles published in authoritative journals in recent 5 years were accepted in priority. Repetitive articles and Meta analysis were excluded. RESULTS:HBV recurrence after liver transplantation is associated with hepatitis B DNA loading dose, invasion of hepatitis B into non-liver tissues, immunosuppressive therapy and viral genovariation. The major prevention and treatment protocol of HBV reinfection after liver transplantation is the combination of anti-hepatis B immunoglobulin and lamivudine, which is economical and efficient. However, long-term administration of lamivudine induces YMDD variation in hepatitis B DNA polymerase, leading to drug resistance even HBV recurrence. Now adefovir dipivoxil is regarded as an effective remedy for YMDD variation. CONCLUSION:Virus variation and HBV recurrence can influence prognosis of HBV-related end-stage diseases after liver transplantation. Prevention and cure approaches are developing. It is important to find an economic, safe, convenient and effective therapeutic regimen. In addition, individualized treatment and the evaluation of risk and advantage should be emphasized.

Objective To explore the electrophysiological characteristics and the expression of mRNA and protein of L-type calcium channel in rat bone mesenchymal stem cells(MSCs). Methods MSCs were isolated,cultured and purified.RT-PCR was used to detect the mRNA expression of ?1C,?1D,?1H,?1S.The protein expression of L-type calcium channel(?1C) was testified by immunohistochamical.Ion currents were recorded in MSCs using whole-cell patch clamp technques.Results CD29,CD44,CD106 expressed in about 93% MSCs and CD14,CD34,CD45 expressed negatively.A high expression of mRNA in ?1C was detected by RT-PCR but no expressions were observed in ?1D,?1G,?1H,?1S.Immunofluorescent double labeling showed an expression of ?1C subunits in MSCs.Moreover,inward currents were recorded in 16 of 36 cells using whole-cell patch clamp techniques.The currents were activated around-30?mV and peaked at 0 to 10?mV and were blocked by nifedipine(10??mol/L).These cells had larger currents with Ba~(2+)(10?mmol/L) in bath solution than with Ca~(2+)(2?mmol/L).Conclusion The results indicated that adult rat MSCs expresse functional L-type calcium channels.It is possible that this channel plays a role on the proliferation and differentiation of MSCs.

Objective To investigate the possible way through which transplanted Sertoli cells support the nerve tissues in vivo.Methods Culture the isolated cells from the fetal mouse cerebral cortex alone(coated the culture plate with poly-D-lysine previously),the control group,or with prepubertal homologous Sertoli cells(planted Sertoli cells beforehand),the coculture group.To observe the morphological changes of the cultured neurons,neural stem cells(NSC) and neuroglial cells every day and compared the differences in their growth between the tow groups.Sertoli cells,neurons and NSC were identified by the expression of Fas ligand,neuron-specific enolase(NSE) and Nestin in the cytoplasm respectively.Results A significant increase of both the survival of neurons(P

Objective To investigate the clinical characteristics of juvenile rectal carcinoma in order to improve the diagnostic level and therapeutic efficacy at the early stage. Methods Clinical data, including clinical manifestations, misdiagnosis, clinical pathology, treatment, and prognosis, from 89 cases of juvenile rectal carcinoma were analyzed retrospectively and compared with those from 440 cases of senile rectal carcinoma. Results ① The main manifestations of juvenile rectal carcinoma included anal and rectal pains, abdominalgia, abdominal distention, and sacrococcygeal pain. ② In juvenile group, carcinomas with distance less than 9 cm and less than 7 cm away from the anal verge amounted to 92.13% and 69.66%, respectively (average distance: 5.43 cm). ③ The average period of misdiagnosis in juvenile group was 5.7 months with a misdiagnosis rate of 97.75%, significantly higher than that in senile group ( P

Objective To understand the cognitive status of urology medical staff about hematuria color.Methods The self-designed questionnaire about color cognition was adopted,and the concentration of hematuria samples were determined.The investigation was carried out in 50 health care workers about their cognition of hematuria color.Results The study found that there were differences in color perception between doctors and nurses,doctors and nurses with different titles,doctors and the standard sample,nurses and the standard sample.Conclusions There are differences in judgment and description of the urine sample color in medical personnel,they are more attempted to judge by virtue of work experience and subjective consciousness.Due to the lack of a uniform and objective standards,the medical staff can not accurately and objectively describe the development of patients' condition,thus patients' recovery was affected.Therefore,a unified objective criteria for clinical reference norms of hematuria should be developed as soon as possible to ensure that patients recover quickly and safely.

Objective To investigate the correlation between the unilateral middle cerebral artery (MCA)stenosis ratio and cerebrovascular reserve(CVR) using computed tomography perfusion (CTP) and CO2 inhalation test in patients with unilateral middle cerebral artery stenosis.Methods CTP were performed in 31 patients who were diagnosed as unilateral middle cerebral stenosis before and after CO2 inhalation.The basal ganglia and radial dimension were selected as interested regions (thickness 8 mm).The value of cerebral blood flow (CBF) was measured and the mean CBF values of all interested regions in each MCA territory were harvested separately.Then the CVR of each MCA territory was calculated according to the following formula:CVR =(CBF after CO2 inhalation test-CBF before CO2 inhalation test)/ CBF before CO2 inhalation test × 100％.Patients were divided into two groups:the severe stenosis group and the moderate stenosis group.The association between the MCA stenosis ratio and the CVR values was investigated.Results In 31 patients,different changes of CBF were found in affected MCA territory after CO2 inhalation.CBF increased in 17 cases,unchanged in 2 cases and decreased in 12 cases.A decreased CVR was detected in 51.6％ of the patients(16/31) and more likely found in the severe stenosis group (13/19) than that in the moderate stenosis group(3/12,P =0.029).The degree of stenosis in MCA was also significantly correlated with the changes of CVR(r =0.423,P =0.018).Conclusions CVR is decreased in some patients with unilateral MCA stenosis and significantly correlates with the severity of stenosis in MCA.

Objective: To evaluate new ischemic brain damage after using protective filter device during carotid artery stenting with diffusion-weighted magnetic resonance imaging (DWI). Methods: A total of 60 patients with carotid stenosis (stenotic rate 50%-95%) were included in this study. They underwent carotid artery stenting and were divided into protective filter device group (n = 30) and none protective filter device group (n = 30). DWI was performed within 24 hours before and after the procedure. The number, size, and location of new cerebral ischemic lesions after the procedure were counted. Results: Circled digit oneDWI was performed within 24 hours after the procedure. A total of 18 patients occurred high-density ischemic cerebral lesions, six of them (20%) were in the protective filter device group and 12 (40%) were in the none protective filter device group. There was no significant difference (P >0.05). Circled digit two A total of 41 new ischemic cerebral lesions were detected, 14 of them were in the protective filter device group and 27 were in the none protective filter device group. The lesions mainly located in the same sides of stents. There was no significant difference in the constituent ratio of the lesion sites between two groups (P > 0.05). Circled digit threeThere were 33 lesions of ≤3 mm diameter, 13 of them were in the protective filter device group and 20 were in the none protective filter device group. There were 8 lesions >3 mm in diameter, one of them was in the protective filter device group and 7 were in the none protective filter device group. There was no significant difference in the constituent ratio of the lesion size between the two groups (P >0.05). Conclusion: Using protective filter device during carotid artery stenting may decrease the occurrence of new ischemic cerebral lesions, especially large large sized lesions. However, new ischemic cerebral lesions may occur during the procedure.