Postoperative atrial fibrillation (POAF) is the most common complication after cardiac surgery, which leads to hemodynamics disorder, prolongs hospitalization and increases mortality. The underlying mechanisms behind POAF are associated with cardiomyocyte injury, sympathetic activation and inflammation. The prediction of POAF is based on correlated index from pre-, intra-and post-surgery. POAF can be treated by rhythm and heart rate control as well as and antithrombotic therapy.

Epidemiologic evidence suggests that diabetes mellitus appears to be negatively associated with abdominal aortic aneurysm formation. Diabetes mellitus may affect the physiological characteristics of the aorta and reduce the prevalence, incidence and expansion rate of abdominal aortic aneurysm. But the impact of diabetes mellitus on the rupture, operative complication and clinical outcome of abdominal aortic aneurysm is unclear. Randomized controlled trials are needed before conclusions can be drawn on the link between development and expansion of abdominal aortic aneurysm and medicines for diabetes. This review aims to analyze updated evidence of the association between diabetes mellitus and abdominal aortic aneurysm.

Objective To investigate the role of HMGA2 expression and epithelial to mesenchymal cell transformation in the regulation of TGF -β1 in human gastric cancer cells .Methods Human gastric cancer cell SGC-7901 was treated by TGF-β1 at different time points(0,3,6,24,48,72 h).We observed the changes in cell morphology of SGC-7901 through the microscope;HMGA2 assay,E-cadherin,Vimentin protein expression wereperformedbyWesternblot;cellinvasioncapabilitywasanalyzedbytranswellchamberassay.Results (1) With the prolonged duration of action of TGF -β1,SGC-7901 changes in cell morphology ,cells gradually be-came longer and larger.(2)In the role of TGF-β1,gastric cancer cells SGC -7901 are varying degrees of HM-GA2 expression and E -cadherin、Vimentin.HMGA2 protein begun to express from 0h to 6hrs.and HMGA2 pro-tein became the strongest at 6hrs and decreased slightly from 24hrs.E-cad protein expression at 0h begun at 3hrs highest expression ,and the reduction started from 3hrs.Vimentin protein was the highest at 48hrs then gradu-ally decreased.Conclusion In the TGF-β1 induced the process of epithelial to mesenchymal cell transforma-tion in gastric cancer cells SGC -7901 ,HMGA2 protein play an important role .HMGA2 can be used as snail pro-moter,combined with Smads ,promotes the process of epithelial -mesenchymal transformation .HMGA2 may also be regulated by Wnt /β-Catenin signaling pathway on growth and apoptosis of tumor cells .

AlM:To analyze and explore the clinical efficacy of strengthening lateral tarsal strip and advancing lower eyelid retractor for the treatment of degenerative entropion with eyelid relaxation
METHODS: Reviewed from January 2011 to January 2013, 21 cases (33 eyes) of degenerative entropion eyelid relaxation were treated with operation of strengthening lateral tarsal strip and advancing lower eyelid retractor ( fornix conjunctival incision ) , the follow-up was 12-24mo, curative effect was observed.
RESULTS: All the patients were cured completely, have no recurrence or overcorrection phenomenon. The cure rate of operation was 100%, there was no complication.
CONCLUSlON: Operation of strengthening lateral tarsal strip and advancing lower eyelid retractor is a safe, reliable, efficient operation to the treatment of degenerative entropion with mode of eyelid relaxation.

Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

Objective To study the changes of ICAM-1 expression in transplanted lung at the early stage after rat pulmonary transplantation.Methods The expression level of ICAM-1 protein and its mRNA in rat transplanted lung after 4 h of ventilation and reperfussion were detected by using immunohisto chemical method(SABC method)and semiquantitative reverse transcriptase polymerase reaction method in the experimental group(n=6).When donor lungs had been flushed with low potassium solution(LPDS) and preserved in 4.C LPDS for 12 h,left orthotopic pulmonary transplantation was performed.In control group (n=6), left pulmonary artery,pulmonary vein and bronchi were fully freed to be naked.Immunohistochemical test results were recorded in negative(-),suspicion(±),faint positive(+),positive(++)and intensive positive(+++).PCR products were separated by agrose gels and the density of the bands were determined by density scanning.Results The stained color of alveolar epithelial cells and pulmonary vascular endothdial cells in the transplanted lung was significantly more intensive in experimental group than in control group(P＜0.01) and the relative density values(ICAM-1/βactin)were also significandy higher in experimental group(0.837±0.044) than in control group (0.442±0.037),P＜0.01. Conclusions ICAM-1 expression in the transplanted lung was up-regulated in the early stage after pulmonary transplantation,which was related with the enhancement of ICAM-1 mRNA.

The taxol s effect of inducing apoptosis is mainly related to its ability to promote tubulin polymerization and prevent tubulin chain depolarization. Drug resistance is the main restriction factor for clinical usage of taxol. Some basic and clinical studies support that poor response to taxol and poor prognosis are related to overexpression of β tubulin Ⅲ.

Objective To explore the efficacy of ligustrazine combined with chemotherapy in patients with relapsed or refractory NHL and the relationships with P-gp expression. Methods 60 Patients were randomized into reversal agent group (ligustrazine + chemotherapy) and control group (chemotherapy) and the outcome was analysed. P- gp expression was analysed by flow cytometry. Subgroup analysis was conducted a ccording to P-gp expression. Results 56 patients could be evaluated. Progression -free survival (PFS) showed no statistical diference in two groups ( P = 0.065 1 ) ; reversal agent group had a higher overall response rate (ORR) than control group(P=0. 048).Subgroup analysis showed 41 patients had P-gp( +). The complete remission rate and complete remission uncertain rate of patients with P-gp( + ) in reversal agent group and control group were 33. 33% and 8. 70% , respectively, reversal agent group had a significant advantage ( P = 0. 048). Patients with P-gp( + ) in reversal agent group had a higher ORR than control group (61. 11% vs 26. 09% ,P =0.024). Kaplan-Meier survival curve and Log-rank test showed patients with P-gp( + ) in reversal agent group had longer PFS than control group (P =0.046 4). Blood pressure in a small number of patients received ligustrazine therapy decreased,and mitigated after reducing the dose. Conclusion Ligustrazine combined with chemotherapy can elevate response rate,prolong PFS with manageable toxicity and correlate to P-gp expression in relapsed or refractory NHL

Acupuncture Points ; Acupuncture Therapy ; Aged ; Anterior Compartment Syndrome ; therapy ; Humans ; Male ; Tibia ; injuries