1.Effect of sintering temperature on the shrinkage and microstructure of dental fluorosilicic mica glass-ceramics with different particle size
Jiang LI ; Xiaogang CAO ; Zhongyi WANG
Journal of Practical Stomatology 1996;0(02):-
Objective:To investigate the effect of sintering temperature on the shrinkage and microstructure of dental fluorosilicic mica glass-ceramic with two different particle size. Methods:Fluorosilicic mica glass powder with average particle diameter of 72.8 ?m was named PO and that with average diameter of 4.5 ?m was named Pm. PO and Pm were molded by cool isostatic pressing technique and then sintered at different temperatures between 600-1 000 ℃. The shrinkage and microstructure of the sintered blocks of PO and Pm were systematically compared with each other. Results:The shrinkage of PO and Pm blocks were all increased as the temperature ascended. However, the shrinkage curves were distinctly different. At the same sintering temperature the shringkage of Pm was larger than that of PO. Moreover, the turning points of temperature (reflecting transition of the sintering mechanisms) of Pm was higher than that of PO. The microstructure observation showed that sintering at 950 ℃ resulted in mica crystal in glass-ceramics and the best compactness of the sintered blocks of both PO and Pm. Conclusion:Sintering glass powder of smaller particle size at 700-850 ℃ may achieve better sintering compactness.
2.Application of platysma muscle flap with five-valve in treatment of severe muscular torticollis
Sanbao YU ; Jinheng JIANG ; Xiaogang SHEN
Chinese Journal of Medical Aesthetics and Cosmetology 2010;16(2):88-89
Objective To introduce a safe and practical approach for the treatment of severe muscular torticollis. Methods In an axis of the spasmodic sternocleidomastoid muscle, the author designed a five-valve flap with two valvae inside, which ended at the mastoid point and inferior clavicular part of sternocleidomastoid muscle, respectively. The arm length of each valve was approximately half of its axis. Each valve was separated bluntly in naked eyes in order not to detach the platysma myoides from the skin, and to release the webbed neck. Under the flap, the thinned and fibrosed sternocleidomastoid muscle and spasmodic neck sheath and superficial cervical vein were easily found. The sternocleidomastoid muscle was disconnected at the median point, and the two ends were retracted, the superficial cervical vein was cut and ligated, the neck sheath was released to uncover the spasmodic cervical artery and nerve. The head was right positioned, and then the surrounded fascial tissues were released under the protection of the arteries and veins. The flap was sutured to cover the arteries, veins and muscles. Results Reasonably good effect was achieved in one case and intermediate effects in other 4 cases. Surgical results were satisfactory. All the flaps were survival with insignificant scar formation. Conclusions Webbed skin deformity in the neck can be corrected by using five-valve plastic surgery of platysma myoides, which is able to cover the exposed nerves and vessels. This procedure prevents the adhesion of the operated area, ensures the blood supply of the distal portion of the flap, and also avoids the damage of other tissues in the flap area.
3.Effect of Anlv Capsule on sodium ion channels in ventricular myocytes of guinea pig
Huimei XU ; Xiaogang JIANG ; Wenjie ZHANG ; Yan LI ; Jiayi REN
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(03):-
Objective:To observe the effect of Aalv Capsule on sodium current in guinea pig ventricular myocytes(INa)for exploring the mechanism of its anti-premature beats.Methods:Whole-cell patch-clamp recording technique was used to record the Aalv Capsule for a single cell of sodium currents in guinea pig ventricular myocytes.Results:There was dose-dependent of Aalv Capsule in block of sodium currents and had a certain amount of use-dependent and time-dependent.Conclusion:The role of Aalv Capsule in blocking sodium current block was one of the mechanisms of its in bearing premature.
5.Isolation and culture of murine embryonic stem cells of Kunming species
Weidong LI ; Xiaogang ZHANG ; Jing CHANG ; Fangping JIANG
Chinese Journal of Tissue Engineering Research 2007;0(21):-
BACKGROUND: Hundreds of mouse embryonic stem cell lines are established presently. Most of these cell lines are collected from 129, C57BL/6J and BALB/C mice. Success rate of creating embryonic stem cell lines with Kunming mice is very low. OBJECTIVE: To explore the method of isolation and culture of murine embryonic stem cells of kunming species to increase the efficiency of establishment of embryonic stem cell lines from Kunming species mouse. DESIGN, TIME AND SETTING: The cell experiment was performed at the Chongqing Key Laboratory of Neurology from April 2006 to June 2007. MATERIALS: Blastocysts of 3.5 days and 4 days from Kunming species mouse were collected. METHODS: The blastocysts of 3.5 days and 4 days from Kunming species mouse were cultured on the fibroblast cell feeder layers for 4-5 days, and the cells from inner cell mass were isolated and subsequently cultured in vitro in 2.5 g/L trypase-0.4 g/L ethylenediamine tetraacetic acid (EDTA) and 1 g/L trypase-0.2 g/L EDTA at room temperature for 2-4 minutes. Embryonic stem cell colony was mechanically straggled. MAIN OUTCOME MEASURES: Colony growth was observed and determined by alkaline phosphatase staining, OCT-4 staining and karyotype analysis. RESULTS: Adherence rate, inner cell mass frequency and cloning efficiency of 4 day post coitum (dpc) embryo were higher than those of 3.5 dpc embryo (P
6.Differentiation of mouse embryonic stem cells into cardiomyogenic cells incubated with cardiomyocytes
Weidong LI ; Xiaogang ZHANG ; Jing CHANG ; Fangping JIANG
Basic & Clinical Medicine 2006;0(01):-
Objective To explore the effect of embryonic stem cells(ESCs) differentiating into cardiomyocytes(CM) by cardiomyocytes.Methods The blastocytes of 3.5 d from Kunming mouse,cultured on mouse embryonic fibroblaste cell feeder layers for 4~5 d,the mouse ESCs were isolated and subsequently cultured.Day 2~3 embryoid bodies(EBs) were derived from ESCs of passage 3 to 5 and then incubated with neonatal cardiomyocyte(CM) to induce into cardiomyocytes and the expression of cardiac specific cardiac troponin-T(TnT) and ?-actin were detected by immunofluoresence.Results The rhythmic beating embryoid bodies were observed on day 3 and reached 93% of rhythmic beating embryoid bodies on 12 day in group 2.All the beating cardiomyocytes derived from ESCs expressed cardiac-specific proteins for TnT and ?-actin,and the group induced by direct cell contact acquired the highest differentiating ratio as 56.5%,and was the highest differentiating ratio compared with other groups(P
7.Treatment of intertrochanteric fractures by proximal femoral nail and proximal femoral nail antirotation: a comparative study
Xiaogang ZHOU ; Zhengrong CHEN ; Shaojun WANG ; Fian DONG ; Nanchun JIANG
Chinese Journal of Trauma 2009;25(3):240-244
Objective To compare the treatment effectiveness of AO/ASIF proximal femoral nail (PFN) and proximal femoral nail antirotation (PFNA) in treatment of intertrochanterie fractures. Methods A retrospective study was done on 233 patients with intertroehanteric fractures treated from August 2004 to December 2006. The patients were divided into PFN group (188 patients) and PFNA group (45 patients) for comparing operative procedures and postoperative functional recovery. Results There was statistical difference in aspects of incision length, blood loss and operation time between two groups. The follow-up for 22.8 months showed excellence rate of 89.9% in PFN group and 91.1% in PFNA group, with statistical difference (X2 = 0.06, P > 0.05). There occurred hip varus in one patient and antirotation nail cutting-out in two in PFN group, which was not found in PFNA group. Conclusion PFN and PFNA are both good choices for treatment of intertrochanteric fractures. Compared with PFN, PFNA has more advantages in reducing operation time and blood loss especially for the eider patients with osteoporosis.
8.Analysis of high risk factors related to early-onset myocardial damage in multiple trauma patients
Weifeng SHEN ; Xiaoyan SHI ; Xiaogang ZHANG ; Guanyu JIANG ; Yingyu FU
Chinese Journal of Emergency Medicine 2005;14(4):269-273
Objective To evaluate potential clinical risk factors for the development of early-onset myocardial damage following multiple trauma (MT), and to determine whether early-onset myocardial damage was caused by the combined effects of thoracic and systemic injury factors in MT patients.Methods A total of 231 patients with MT over the last 3 years were retrospectively reviewed. With myocardial damage being a dependent variable and other twenty factors being independent variables, univariate and multivariate logistic regressions were applied to investigate the risk factors for early-onset myocardial damage and to identify the association of thoracic and systemic risk factors with early-onset myocardial damage.Results Multivariable logistic regressions showed that acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ ) score ≥10, injury severity score (ISS) ≥ 25, shock index ≥ 2,coexisting chest trauma, abbreviated injury scale (AIS) of chest≥3, and hypoxia time ≥ 0.5 h were risk factors. The risk of earlyonset myocardial damage following MT obviously increased when thoracic and systemic injury risk factors were coexisting.Conclusion Our results indicated that thoracic injury combined with systemic injury increased the overall risk of early-onset myocardial damage following MT. Prospective validation of these findings in other clinical settings is warranted.
9.Research on CXCL12/CXCR4 biological axis and idiopathic pulmonary fibrosis
Hongxia CUI ; Yizhong FENG ; Zhenlun GU ; Xiaogang JIANG ; Ciyi GUO
Chinese Pharmacological Bulletin 2010;26(3):298-301
Idiopathic pulmonary fibrosis(IPF), with unknown pathogeny, is an interstitial lung disease.The pathological features are diffuse epithelial-cell lesion, fibroblast proliferation, myofibroblast differentiation and excessive extracellular matrix deposition.CXCR4 is the predominant chemokine receptor on fibrocytes;CXCL12 is the only ligand of CXCR4.A large number of studies have shown that CXCL12/CXCR4 biological axis plays an important role in the pathogenesis of idiopathic pulmonary fibrosis.Under the regulation of hypoxia, HIF-1α and PI3K-Akt-mTOR path, CXCL12/CXCR4 biological axis promotes lung fibroblast proliferation, myofibroblast differentiation and extracellular matrix deposition, resulting in development and progression of IPF.
10.The inhibitory effect of oridonin on human pancreas adenocarcinoma SW1990 cells
Fang SONG ; Yizhong FENG ; Xiaogang JIANG ; Zhenlun GU ; Ciyi GUO
Chinese Pharmacological Bulletin 2010;26(2):240-243
Aim To study the inhibitory effects and its mechanisms of oridonin on human pancreas adenocarcinoma SW1990 cells.Methods Cell growth inhibition mediated by oridonin on SW1990cells was measured by MTT assay.The morphological changes were observed by Hoechst33258 fluorochrome staining and electron microscope.Cell cycle and apoptosis rate were analyzed by flow cytometry. The molecular mechanisms involved in the effects of oridonin on SW1990 cells were studied by RT-PCR.Results The growth of humen pancreas adenocarcinoma SW1990 cells was significantly inhibited by oridonin.Apoptosis morphological changes about chromatic agglutination and nuclear condensation were detected by Hoechst 33258 fluorochrome staining and electron microscope in oridonin treated SW1990 cells."Sub-G_1" phase peak and G_2/M growth arrest werer found with flow cytometry.The upregulating mRNA expression of p21 and downregulating mRNA expression of survivin were detected by RT-PCR.Conclusion The inhibitory effect of oridonin on human pancreas adenocarcinoma SW1990 cells through induced apoptosis and G_2/M growth arrest and the mechanisms may be through surviving-p21 co-regluation pathway.