This review addresses the association of "Chinese Journal of Biotechnology" and the development of biotechnology in China in the past 30 years. Topics include relevant awards and industrialization, development of the biotechnology discipline, and well know scientists in biotechnology, as well as perspectives on the journal.
Biotechnology ; China ; History, 20th Century ; History, 21st Century ; Periodicals as Topic ; history

Human LAK cells induced from peripheral blood mononuclear cells with recombinant interleukin2 (rIL-2) incubation, can be cloned in semisolid medium and proliferated well in liquid cultures containing rIL-2. Phynotypie analysis by flow cytometry showed that these cloned LAK cells were T_3(+), T_4(-), T_8(+), Tac(+), DR(+) and NKH1(-)/(+). Cultured with 1?10~5/ml normal bone marrow mononuelear cells (BMMNC)for 0.5-hr prior to semisolid culture system for CFU-GM, the cloned LAK ceils at 2?10~5/ml showed inhibitory effect on CFU-GM that the colonies were decreased by 43.8% of control though 0.5 or 1?10~5/ml LAK cells did not. Morever, a dose-dependent inhibition of CFU-GM was noted when LAK cells pre-inbated with BMMNC for 4-hr in doses of 0.5 -4?10~5/ml that the inhibitory rates of CFU-GM were 58.8, 67.5, 88.8 and 96.3% at0.5, 1, 2, 4?10~5/ml LAK cells addition respectively. Using double-layer agar cultures, inhibitory activity for CFU-GM was also observed only when LAK cells at 4?10~5/ml without contacted with BMMNC. No significant difference of inhibitory effects on CFUGM growth was found between autologous and allogenie clotted LAK cells under same condition. These data suggeste that LAK cells inhibited growth of myeloid progenitor cells without MHC-restriction and the mechanism might involve cell-to-cell interaction and/or some soluble factors secreted by LAK cells.

OBJECTIVE:To compare the relative bioavailability between2loratadine tablets(LORAX and LORAS)in human body.METHODS:22healthy male volunteers were enrolled to receive oral single dose(20mg)of either LORAX or LORAS tablets by crossover way,plasma concentrations of loratadine were determined by HPLC and pharmacokinetic param-eters were calculated by3p97program.RESULTS:The pharmacokinetic parameters of LORAX vs.LORAS were the fol-lowing,t 1/2Ke (3.03?0.98)h vs.(2.65?0.81)h;C max (8.59?6.70)vs.(9.59?8.22)ng/ml;t max (1.08?0.41)h vs.(1.21?0.28)h;AUC (0～t) (27.29?24.54)vs.(27.92?20.64)(ng?h)/ml.The relative bioavailability of LORAX was(100.63?14.27)%.CONCLUSION:2loratadine tablets were bioequivalent.

Sepsis is systemic inflammatory response syndrome(SIRS)associated with the presence of pathogenic microorganisms or their toxin.Lipopolysaccharide(LPS)is an important component of the outer membrane of Gram negative bacteria and has a pivotal role in inducing Gram negative sepsis.Macrophages play an essential role in infection and inflammation.Specific recognition of LPS is of particular importance in the defense system and pattern recognition receptors(PRR)have been considered to be important in initial steps for cellular recognition of LPS and consequence initiation of LPS responses.In the past few years,intense research in the fields of PRR and their recognition mechanism has been achieved.In this review,we attempt to expound recent research advances of macrophage PRRs for LPS recognition and their mechanism.

By analyzing the characteristics of maternal abdominal ECG (Electrocardiogram), a method based on wavelet transform and matched filtering is proposed to detect the R-wave in fetal EGG (FECG). In this method, the high-frequency coefficients are calculated by using wavelet transform. First, the maternal QRS template is obtained by using the arithmetic mean scheme. Finally, the R-wave of FECG is detected based on matched filtering. The experimental results show that this method can effectively eliminate the noises, such as the maternal ECG signal and baseline drift, enhancing the accuracy of the detection of fetal ECG.
Abdomen ; Algorithms ; Electrocardiography ; Female ; Fetus ; Humans ; Pregnancy ; Wavelet Analysis

Since 1960s,total parental nutrition(TPN) has had a great development,while complications after long term of TPN has also been recognized.This article reviews intestinal permeability changes after TPN,its possible mechanism and some recent research progresses.

Objective To explore the prevalence and related factors of female sexual dysfunction(FSD) in women with diabetic.Methods Female Sexual Function Index(FSFI) was used to investigate the prevalence of FSD in 336 women with diabetes.Logistic analysis was conducted between diabetic women with FSD and without FsD for related factom of diabetic FSD.Results Two hundred and fourty-four patients were found to have FSD.The prevalence was 72.6% .The Logistic analysis showed that FSD was significantly relaed to diahetic course and diabetic neuropathy.Conclusion The prevalence of FSD in diabetic women is significandy greater than non-diabetic women.Diabetic course and diabetic neuropathy play an important role in the development of FSD in diabetic women.

Objective To investigate the related factors and preventive measures of nasopharyngeal massive hemorrhage in patients with nasopharyngeal carcinoma after radiotherapy. Methods Clinical data of 43 nasopharyngeal massive hemorrhage patients with nasopharyngeal carcinoma after radiotherapy were analyzed retrospectively. Results Thirty-nine cases(90.7 %) had abroad skull base invasion due to tumor, 36 cases (83.7%) received high dose of radiation, and 13 cases (30.2 %) were accompanied by diabetes. Conclusion Tumor location is the major factor for nasopharyngeal massive hemorrhage, while radiation dosage, fractionation patterns and diabetes are the secondary factors. The measures in cluding hyperfractionation, overall dose control and blood sugar regulation should be taken for the high risk patients. Prevention is more important than salvage.

Objective: To study the quick extraction and determination of icariin in Herba Epimedii.Methods: The orthogonal design and ASE300 system were used to study the extraction method of icariin.The content of icariin was determined by HPLC.The methanol water glacial acetic acid(60∶40∶0.25) was used as a mobile phase. The flow rate was 1.0ml/min,the detection wavelength was 270nm and the column temperature was 41℃.Results: The optimal extraction conditions were: 50% alcohol and extracting one times for twenty minutes each time at 70℃.The extracting rate of icariin was 93.15%.The RP-HPLC for determining method is simple,rapid and accurate.Conclusion: The extraction and determination methods are practicable for icariin in Herba Epimedii.

Objective:To study the microwave-assisted extracting method for magnolol and honokiol in Magnolia offcinalis Rend et Wils.Methods:The orthogonal design was used to study the extraction method of magnolol and honokiol.The contents of them were determined by HPLC.The methanol water glacial acetic acid(85:15:0.25)was used as a mobile phase.The flow rate was 1.0ml/min,the detection wavelength was 294nm and the column temperature was 23℃.Results:The condition of 75%ethanol with the middling power of microwave,extracting for 4 minutes and ratio of materials to liquid 1:40 was the optimal extracting method.The extracting rate of honokiol and magnolol was 92.33%,73.59%.The RP-HPLC for determining method was simple,rapid and accurate.Conclusion:The extracting and determining method was practicable for magnolol and honokiol in Magnolia offcinalis Rend et Wils.