Primitive neuroectodermal tumor (PNET) is a kind of malignant tumor, which most often affects children and adolescents. The incidence of PNET has risen recently. PNET has low early diagnosis rate and poor treatment effect. With the application of RT-PCR and FISH in pathological diagnosis, and MRI and PET-CT in imageological diagnosis, as well as the implementation of target treatment and multidisciplinary integrated treatment concept in the treatment, the pathogenesis, diagnosis technology and treatment means of of PNET have made remarkable progress.

Platelets, small pieces of cytoplasm with biological activity, split and fall off the megakaryocytes and mature from the bone marrow. After stimulated, platelets produce nitric oxide to inhibit their own activation and aggregation. Pathologically, the injury of endothelial cells activates platelets and changes their functions. The release of inflammatory mediators and cytokines induces and enhances the development and progression of atherosclerosis, and thereby promotes the occurrence of erectile dysfunction. Besides, platelets and their related functional parameters may serve as important indicators in the diagnosis and treatment of erectile dysfunction.
Atherosclerosis ; etiology ; Blood Platelets ; physiology ; Cytokines ; metabolism ; Endothelial Cells ; Erectile Dysfunction ; etiology ; Humans ; Male ; Nitric Oxide ; biosynthesis ; Platelet Activation

Blood blister-like aneurysms mostly originate from the non-bifurcation sites of the internal carotid artery.They have the characteristics of the aneurysm wall fragile and wide neck.Currently they were treated with neurosurgical procedures and endovascular interventional techniques.This article reviews the advances in the treatment of blood blister-like aneurysms of the internal carotid artery.

100 RU), and 19 such as biotae folium et ramulus, lycium bark and rhubarb had higher contents of active substances that of specific affinity to CpG DNA. CONCLUSION: It is feasible to screen traditional Chinese medicine by biosensor technology. Nineteen kinds of TCM contain the active non-tannin substance with specific affinity to CpG DNA.

OBJECTIVE:To discuss the significance of prescription analysis in Chinese drugs dispensing and to probe into the profound connotation of the dispensing of prescriptions of traditional Chinese medicines.METHODS:Take the basic theory of Chinese medicine as the basis,the importance of prescription analysis in the dispensing of traditional Chinese medicines were analyzed from aspects of the patients' clinical diagnosis,medication intention as well as the function and properties of drugs etc.RESULTS:8 essential factors were found to be involved in prescription analysis,which shed new light on the omnidirectional pharmacy care provided for patients.CONCLUSION:The key for Chinese drugs dispensing lies in the prescription analysis.

Objective To explore the clinical value of 18F-FDG PET/CT in the diagnosis of cardiac neoplasm.Methods Between January 2007 and December 2011,18F-FDG PET/CT was performed in 8649 patients in our hospital,and 14 (0.16％ ; 11 males,3 females,average age 46.9 (35-68) years) patients were diagnosed with cardiac neoplasm.18F-FDG PET/CT data of those 14 patients were retrospectively analyzed.Final diagnoses were confirmed by either histopathology (n =5) or follow-up (n =9).Results Accurate tumor localization and diagnoses were made by 18 F-FDG PET/CT in all 14 patients.Among them,13 were malignant (2(14.3％,2/14) primary and 11(78.6％,11/14) metastatic tumors (8 HCC,2 lung cancer,1 esophageal cancer) ; SUVmax 3.2-10.7) and 1 (7.1％,1/14) was benign (myxoma).Conclusion 18F-FDG PET/CT is useful for the detection and staging of primary malignant and metastatic cardiac neoplasms.

ObjectiveTo investigate genetic polymorphism of 3 STR loci in Bouyei ethnic group in Guizhou.Methods The DNAs of 101 healthy unrelated Bouyei individuals in Guizhou were extracted using chelex-100 method and were multiplex amplificated by PCR technique and the denatured PAGE was used, the PCR product was typied using silver stain method. The data was statistically analysed by modified-powerstat software package. Results A total of 21 alleles and 50 genotypes were observed in the 3 loci and the distributions of these genotypes were consistent with the law of Hardy-Weinberg equilibrium. The heterozygosity(H) of CSF1PO, TPOX and TH01 loci were 0.7287,0.5423 and 0.6904; the polymorphism information content(PIC) were 0.6805,0.4479 and 0.6426; the discrimination power(DP) were 0.8782,0.7361 and 0.8563 and the probabilities of paternity exclusion (PPE)were 0.6148、0.3643 and 0.5737 respectively. Conclution CSF1PO and THOX are highly genetic polymolphism, which is valuable for population genetic research and forensic individual identification. The distrbution of gene frequencies and their area of ethnic groups have parallel relation.

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

Objective To investigate the relationship between the ankle-brachial index (ABI)and the extent of coronary artery stenosis and the left ventricular function in elderly patients with coronary heart disease (CHD)with metabolism syndrome (MS). Methods A total of 140 patients with coronary artery disease was study subjects. In which, 66 cases complicated MS (MS group), 74 cases without MS (non MS group). ABI and the left ventricular function was examined in an patients. Results ABI was 0.90±0.32 in MS group, and 1.03±0.26 in non MS group, there was significant difference between two groups(P=0.015). Compared with non MS group, significant lower ratio of ABI in MS group [43.9%(29/66) vs 27.0%(20/74)] (P=0.038); whereas much more 3-vessel disease in ABI≤0.9 patients compared with those of ABI > 0.9 patients [86.3%(25/29) vs 37.8%(14/37) ], significant lower of the left ventricular function in ABI≤0.9 patients compared with those of ABI >0.9 patients (P<0.05). Conclusion There is more severe of coronary artery stenosis in ABI≤0.9 patients for CHD with MS, more attention should be payed to CHD patients combined with MS.

Objective To investigate the association of inducible nitric oxide synthetase (iNOS) expression with the expression of mutation type p53 (mt p53) in prostatic carcinoma (PCa). Methods The expression levels of iNOS and mt p53 were in 28 PCa specimens and 10 cases of benign prostatic hyperplasia (BPH) were detected by immunohistochemical method. Results The expression levels of both iNOS and mt p53 in PCa were higher than those in BPH. The expression of iNOS was related to the expression of p53 protein. Conclusion iNOS and mt p53 may play a role in the pathogenesis of PCa.