Objective:To research the quality standard for Xilei San. Methods:Imdigo naturalis and calculus bovis were identi-fied by TLC and their contents were determined by HPLC, and Borneolum syntheticum was determined by GC. Results: Indirubin showed good linearity over the concentration range of 0. 043-4. 255 0 μg(r=0. 999 2) with the average recovery of 97. 9%(RSD=1.5%,n=6). Bilirubin showed good linearity over the concentration range of 9.968 ×10 -4-9.968 ×10 -2 μg(r=0.999 9) with the average recovery of 95.3%(RSD =0.55%, n =6). Borneolum syntheticum showed good linearity over the concentration range of 0. 010 3-0. 826 6 mg(r=0. 999 8) with the average recovery of 94. 9%(RSD=3. 2%,n=6). Conclusion: The method is accurate with good reproducibility,which can be used as the quality control for Xilei San.

Objective To explore the levels of T lymphocyte subsets in asthmatic children and discuss their relationship.Methods 30 children with acute asthma attacks were divided ito disease control(bronchopneumonias)group(25 cases)and healthy control group(14 cases).Flow cytomerty was used to analyze the T lymphocyte subsets in them.Results CD 4+ and CD 4+/CD 8+T cell of the patients of the maderate to serere degree were both significantly higher than those in normal control.Conclusion(1)CD 4+ T cell plays an important role in asthma chronic airway inflammation.(2)There were no significant difference in the level of CD4+ T cell,CD8+ T cell,CD4+/CD8+ T cell between the patients with asthma of mild degree and those of moderate or severe degree group,but CD 8+ T cell a little higer in the latler patlents,which still needs further research.

Objective: To intvestigate the effects of processing on contents of matrine and oxymatrine of Radix Sophorae Flavescentis. Methods: The determinations of matrine and oxymatrine were carried out by dual wavelength TLC scanning. Results: There were obvious differences between the crude and the different processd products of Radix Sophorae Flavescentis in matrine and oxymatrineConclusion: The processing temperature, material, wine and vinegar can make the contents of matrine and oxymatrine of Radix Sophorae Flarescentis change.

Object To investigate the effects of different processed on content of matrine in the root of Sophora tonkinensis Gapnep. Methods The determination of matrine was carried out by double wavelength TLC scanning for different processed products. Results There were obviously differences between the crude and kinds of different processed products of matrine in the root of S. tonkinensis. Conclusion The different processing methods and adjuvant materials can make the contents of matrine in the root of S. tonkinensis change before and after processing.

Purpose:To evaluate the results of combination chemotherapy with MxFL[mitoxantrone(MIT),Fluaroracil (5 FU),Leucovorin (CF)]in the treatment of metastatic breast cancer.Methods:From 1993 through 1998,41 patients with metastatic breast cancer were enrolled in this study, Twenty six patients had no prior chemotherapy and 15 were had prior chemotherapy in nineteen patients estrogen receptor (ER) was positive. MIT 12 mg/m 2 by was given intravenous titrate the first day; continuous 5 FU 320 mg/m 2 by intravenous titrate the first day;continuous 5 FU 320 mg/m 2 by intravenous titrate was given for one hundred and twenty hours during the first day to the fifth day; at the same time, CF 50 mg/m 2 by intravenous titrate, once every twelve hours for five days. Repeated every three weeks. Results:Complete response was observed in 11 patients and partial response was observed in 19 patients with an overall response rate of 73.2%. The median response duration was 17 months and the median survival period was 23 months. The dose limiting toxicity was neutropenia which was seen in 85.4% of the treated cases (19.5% in grades Ⅲ and Ⅳ). Stomatitis was observed in 9.8% of the patients and local venous toxicity was observed in 12.2% of the patients. Conclusions:A high response rate is obtained in metastatic breast cancer treated by MxFL. Such treatment may be used as first line chemotherapy for metastatic breast cancer.

Objective To investigate the gene expression change of TNF-α,IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury.Methods A total of 24 adult male SD rats were randomly divided into four groups:sham group and 3 groups with brain ischemia reperfusion of 3h,6h and 12h.Real-Time PCR was used to analyze the gene expression ofTNF-α,IL-6 and IL-1 β at 3h,6h,and 12h after reperfusion.Results In the sham group,the mRNA expression levels of TNF-α,IL-6 and IL-1 β in were low,but increased immediately after brain ischemia injury and decreased gradually thereafter.The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak (P ＜0.01) then significantly decreased at 12h after reperfusion.The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h (P＜0.01),and significantly decreased at 12h compared with 6h (P＜0.01).The gene expression of IL-1 β mRNA at 3h after reperfusion was significantly increased,peaked at 6h (P＜0.01) and significantly decreased at 12h (P ＜0.01).Conclusion The gene expression levels of TNF-α,IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak,which suggested that the gene expression change of TNF-α,IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury.

Objective To compare the results of clinical treatment of shoulder and neck pain in patients with and without upper cervical degeneration. Methods Fifty two patients with diagnosed shoulder and neck muscular fasciitis(with high tension and tenderness on the trapezius) were divided into 3 groups: group A,with upper cervical degeneration(16 cases);group B,with upper cervical degeneration(15 cases),and group C,without cervical degeneration(21 cases).There were no significant difference in gender,age and course of disease among the 3 groups.Patients in 3 groups were regularly treated by naprapathy and electric therapy(intermediate frequency).Cervical traction and manipulation was added to the patients in group A.Treatment for 3 groups lasted for 2 weeks.The Visual Analog Scale(VAS) scores and Odom criteria before and after treatment were used for determining the results of clinical treatment. Results The VAS scores after treatment were significantly improved in group A and group C as compared with that in group B(P=0.000).Odom criteria of group A(93.8%) and group C(95.2%) were excellent and good without significant difference(P=0.692),but they were significantly higher than that of group C(P=0.001). Conclusions Patients with shoulder and neck pain accompanied by upper cervical degeneration(narrowing of intervertebral foramen) should be treated with cervical traction and manipulation beside the routine therapy.

Objective:To investigate the expression of CD55 in triple-negative breast cancer (TNBC) and its correlations with prognosis.Methods: The expressions of CD55 was detected by immunohistochemistry Streptavidin-Peroxidase method in 100 cases of triple-negative breast cancer and 50 cases of adjacent-carcinoma tissues, with clinical and pathological features and follow-up data, the Cox model analysis was established and to analyze the prognostic factors. Results: The positive expression of CD55 was 56.0% in the tissues from triple-negative breast cancer, whereas its positive rate was 8.0% in the corresponding adjacent breast tissues, with statistical significance (x2=32.0,P<0.05). The expression of CD55 was significantly related with tumor size, clinical stage and axillary lymph node metastases (x2=7.404,x2=8.688,x2=4.802;P<0.05); the expression of CD55 did not show correlation to age, and histologic grade. Multivariate analysis showed that the age, clinical stage, CD55 protein expression and axillary lymph node metastasis were the independent risk factors affecting the 5-year disease-free rate of patients and that clinical stage and axillary lymph node metastasis were the independent risk factors affecting the 5-year overall rate of patients.Conclusion: The expression of CD55 might be related to the development and prognosis of triple-negative breast cancer. The expression of CD55 may be used as one of the indicators in determining the progress and prognosis of TNBC.

Objective Retrospectively discuss the effect of deep anesthesia on the post-operative complications with CPB. Methods All of the chosen patients including anesthesia group (30 cases) and deep anesthesia (30 cases) had the heart surgery under general anesthesia and CPB with the age of (56?6.4), the time of CPB (148?23.6) minutes, Medtronic membrane oxygenator, 4:1 cold blood cardioplegia, the flo of 2.0～2.4L?min -1 ?m -2 .The relative markers: operatively, the mean artery pressure (MAP), end tip oxygen saturation (SpO_2),the mean quantity of regitine (Regitine);postoperatively, the mean base-excess (BEpost), the mean quantity of 5% sodium bicarbonate per kilogram (NaHCO_3post),oxygen index, the level of blood glucose ,the duration of mechanical ventilation and intensive care unit stay , wound healing up, and the mortality. Results With deep anesthesia, the patients had more stable hemodynamic reserve operatively and significant improvements in the balance of acid-base, the lung function, the blood sugar , the wound recovery and the mortality. Conclusion The complication of postoperation with CPB is affected by the degree of anesthetic depth, and the proper depth of anesthesia can reduce the post-operative morbidity and mortality.

Objective: To study the effect of processing on the content of organic acids in the different processed products of Fructus Crataegi. Methods: The determinations of the crude Fructus Crataegi, five kinds of different processed products and newly fried product were carried out by titration. Results: There were significant differences in organic acid contents among different processed products and the crude. Conclusion: Different processed method and temperature can effect the contents of organic acid on Fructus crataegi.