Objective: To analyze the imaging findings, diagnosis and treatment of central neurocytoma. Methods: The clinical information of 7 cases of central neurocytoma in Second Hospital of Hebei Medical University from January 2005 to March 2013 was retrospectively analyzed. The surgical experience was summarized, and the related literatures were reviewed. Results: Of 7 cases, 3 were females and 4 were males; 6 had tumor in anterior 2/3 of the ventricle, 5 originated from septum pellucidum around monro foramen, 1 originated from lateral ventricular wall. The MRI showed that the lesions were mostly hypointense and displayed mixed signals on T1WI images, mainly hypointense and slightly hyperintense signals on T2WI images; most of neurocytomas showed heterogeneous enhancement on dynamic contrast-enhanced MRI images. Six cases underwent gross total resection, 1 case underwent subtotal resection. Two cases received postoperative radiotherapy, 1 case received adjuvant chemotherapy. One case died postoperatively in hospital. Five cases were followed-up, and 1 case was lost to follow-up. The follow-up time was from 15 to 25 months. Four cases had satisfying prognosis, 1 case had hemiplegic paralysis, and no cases had recurrent tumor. Conclusion: Microneurosurgery is the best treatment for central neurocytoma, mastering the technique and key points of operation can achieve significant clinical effectiveness and improve the prognosis. Copyright © 2013 by TUMOR.

Objective To investigate the effect of dynamic regulation of negative pressure values in vacuum sealing drainage (VSD) on healing of soft tissue wounds.Methods From January 2013 to December 2015,115 patients were treated at our institute for open soft tissue defects of Gustilo-Anderson type ⅢaⅢc.They were 69 males and 46 females,aged from 17 to 76 years (average,44.7 years).They were divided into 4 groups to be subjected to different negative pressure values.Group A (n =29) was subjected to negative pressure values from-40 to-20 kPa,group B (n =29) to values from-80 to-60 kPa,group C (n =29) to values from-40 to-20 kPa for the first 72 hours followed by values from-80 to-60 kPa,and group D (n =28) to values from-60 to-80 kPa for the first 72 hours followed by values from-40 to -20 kPa.The 4 groups were compared in terms of drainage volume,wound shrinking,cleaning time,healing time and complications.Results The drainage volumes on the 7th day in groups B and D (4.89 ± 0.66 mL/cm2 and 4.33 ± 0.96 mL/cm2) were significantly higher than those in groups A and C (2.90 ± 0.67 mL/cm2 and 2.99 ±0.56 mL/cm2);the wound shrinking areas on the 14th day in groups B and D (14.16 ± 1.77 cm2 and 13.84 ± 1.65 cm2) were significantly larger than in groups A and C (12.57 ± 1.92 cm2 and 10.95 ± 1.37 cm2) (P ＜ 0.05).The cleaning time in group A was significantly longer than in the other 3 groups (P ＜ 0.05).The healing time in groups A and C was longer than in groups B and D (P ＜ 0.05).The incidences of pain in groups B and C were significantly higher than in groups A and D (P ＜ 0.05).The incidences of infection and blocking in group A were higher than in the other 3 groups (P ＜ 0.05).Conclusion Dynamic regulation of negative pressure values from-80 to-60 kPa for the first 72 hours followed by values from-40 to-20 kPa is an optimal choice for VSD management of soft tissue defects due to adequate drainage,obvious wound shrinking,quick cleaning and healing,and limited complications.

BACKGROUND: Cryotherapy of acute soft tissue injury has been widely used in clinical practice.OBJECTIVE: To observe the histological changes and treatment effect of different cryotherapies on the rats' acute damage of soft tissue. METHODS: Neonatal Wistar rats were randomized to normal, model, intermittent cryotherapy and continuous cryotherapy groups. Models of acute damage of soft tissue were established in model, intermittent cryotherapy and continuous cryotherapy groups. In intermittent cryotherapy group, the injury was treated by intermittent cryotherapy with ice bag at 4 °C; in the continuous cryotherapy group, the injury was treated by continuous cryotherapy with ice bag at 4 °C; the model group was not treated. Histological changes were observed at 48 hours. Injury degree was evaluated using injury symptom index.RESULTS AND CONCLUSION: Compared with model group, the scores of injury symptom index and histology were lower, interleukin-1β expression was reduced, and transforming growth factor-β1 (TGF-β1) expression was increased in intermittent cryotherapy and continuous cryotherapy groups (P < 0.05). Compared with intermittent cryotherapy group, the scores of injury symptom index and histology were reduced (P < 0.05), interleukin-1β expression was reduced (P < 0.05), and TGF-β1 expression was increased in continuous cryotherapy group (P < 0.05). Results demonstrated that cryotherapy can cure the acute damage of soft tissue by reducing interleukin-1β expression and raising TGF-β1 expression. Continuous cryotherapy is superior over intermittent cryotherapy.

The thiamine and nicotinic acid content of the husked rice (whole rice) samples from 9 representative varieties of paddy collected from Jiangsu and Zhejiang provinces were analyzed, and the effect of degree of milling on the content of these two vitamins were studied. The paddy samples were husked with wooden mill and the husked rice samples were milled with laboratory rice mill to 5 grades, namely, "96", "94", "92", "90" and "88" percent extraction (by 96% extraction we mean 96 grams of finished rice were derived from 100 grams of husked rice), and the thiamine and nicotinic acid content of each sample were determined. It is found that the thiamine and nicotinic acid content of the rice of different variety may vary considerably, and both were decreased with the increase of degree of milling. Generally, above the "94" grade, the loss of thiamine is higher than that of nicotinic acid, while down from the "92" grade, less thiamine and more nicotinic acid were lost. The rate of decrease in nicotinic acid content is relatively irregular as compared with that of thiamine, and different varieties are affected differently by the milling process.

The study is aimed to confirm the silencing efficiency of the vector in human hepatocellular liver carcinoma cell line (HepG2), and observe effects of AMPKgamma silencing on the AMPK stimulating activity and lipid synthesis of cordycepin (CCS), a natural product with known AMPK activating function. The downregulating efficacy of siRNAs on AMPKgamma expression was confirmed in our previous study. The double stranded shRNA Oligo was ligated to lentivirus vector and verified by sequencing. The lentiviral which can effectively inhibited protein expression levels of AMPKgamma was selected by Western blotting, and the regulation of CCS on protein expression of AMPKgamma and p-AMPK in AMPKgamma silence cells were detected by Western blotting analysis. The lipid accumulation in cells was observed by Oil-Red O stain and cells were collected for the estimation of cholesterol (TC), triglyceride (TG). The results showed that the lentiviral vector carrying a shRNA targeting the AMPKgamma gene was successfully constructed. Western blotting analysis confirmed that GR085 had the highest interfering efficiency. Treatment with CCS can significantly increase the levels of phospho-AMPK in normal cells, and the level of TC, TG was reduced, but in AMPKgamma silence cells the effects of CCS on AMPK activation and lipid synthesis were almost completely abolished without changing the expression levels of total AMPK or AMPKgamma protein. In conclusion, the AMPKgamma gene may be related to AMPK activation and intracellular lipids regulation by CCS.

ObjectiveTo explore the efficacy and side-effects of reboxitine on post-schizophrenic depression.Methods30 patients with post-schizophrenic depression according CCMD-Ⅲ accepted reboxitine 4～8 mg/d in addition to antipsychotic drugs for 6 weeks. Efficacy and side-effects were determined with Hamilton Depression Scale (HAMD), Brief Psychiatric Rating Scale (BPRS), Scale for Assessment of Negative Symptoms (SANS), Treatment Emergent Symptom Scale (TESS) before and 2, 4, 6 weeks after treatment. ResultsThe score of HAMD were obviously improved after the treatment(P<0.01), as well as that of negative psychotic symptoms in BPRS and TESS. Slight symptoms that may be associated to the Reboxitine were obvioused seldom, and all recovered without any treating. ConclusionLow-dose rebositine is effective on post-schizophrenic depression with few side-effects and good compliance.

Acute Disease ; Adolescent ; Adult ; Aged ; Child ; Female ; Follow-Up Studies ; Humans ; Ivermectin ; analogs & derivatives ; poisoning ; Male ; Middle Aged ; Retrospective Studies ; Young Adult

Objective To study the abnormal expression of P-AKT in follicular thyroid carcinoma and its relationship with angiogenesis. Methods MVD and expressions of P-AKT, VEGF in 40 specimens of FTC tissues were detected by immunohistochemical PV6000 method. Results The expression rate of P-AKT in FTC was higher than that of non-FTC tissues. The result was of statistical significance (P<0.001). There were correlations between the MVD of FTC tissue and the expression of P-AKT and VEGF (P<0.05). The MVD of P-AKT positive FTC tissue was higher than that of negative tissues (P<0.05). The MVD of VEGF positive FTC tissue was also higher than that of negative tissues(P<0.05). There were correlations between the expression of P-AKT and VEGF(P<0.05). Conclusion The hyperexpression of P-AKT played an important role in the secondary deposits and development of human follicular thyroid carcinoma. AKT/VEGF pathway may exist in FTC, and AKT is the key factor in the pathway which regulated the expression of VEGF.

Bone growth factors play an important role in the process of bone metabolism and perform a variety of physio -logical functions such as regulating bone growth , differentiation and remodeling .Meanwhile , these cytokines are associated with orthopedic disorder and therapy .The curative effect of the overturned sartorius iliac flap in repair of acetabular defect in developmental dysplasia of the hip proves to be good , but mechanism between the overturned flap and the surrounding bone tissue is unclear .This review briefly summarizes the functions and applications of several major cytokines in bone for -mation and remodeling , which may provide new ideas and methods for illuminating the mechanism of these cytokines during the repair of the acetabular defect .