1.Image quality assessment of pre-processed and post-processed digital panoramic radiographs in paediatric patients with mixed dentition
Isti Rahayu SURYANI ; Natalia Salvo VILLEGAS ; Sohaib SHUJAAT ; Annelore DE GRAUWE ; Azhari AZHARI ; Suhardjo SITAM ; Reinhilde JACOBS
Imaging Science in Dentistry 2018;48(4):261-268
PURPOSE: To determine the impact of an image processing technique on diagnostic accuracy of digital panoramic radiographs for the assessment of anatomical structures in paediatric patients with mixed dentition. MATERIALS AND METHODS: The study consisted of 50 digital panoramic radiographs of children aged from 6 to 12 years, which were later on processed using a dedicated image processing method. A modified clinical image quality evaluation chart was used to evaluate the diagnostic accuracy of anatomical structures in maxillary and mandibular anterior and maxillary premolar region of processed images. RESULTS: A statistically significant difference was observed between pre and post-processed evaluation of anatomical structures (P < 0.05) in the maxillary and mandibular anterior region. The anterior region was found to be more accurate in post-processed images. No significant difference was observed in the maxillary premolar region (P>0.05). The Inter-observer and intra-observer reliability of both pre and post processed images were excellent (>0.82) for anterior region and good (>0.63) for premolar region. CONCLUSION: The application of image processing technique in digital panoramic radiography can be considered a reliable method for improving the quality of anatomical structures in paediatric patients with mixed dentition.
Bicuspid
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Child
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Dentition, Mixed
;
Diagnosis
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Humans
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Methods
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Radiography
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Radiography, Panoramic
2.Viability and DNA Damage of Buccal Mucosa Cells in Patients Exposed to Panoramic X-ray
Ryna Dwi Yanuaryska ; Afit Aditya Atmoko ; Isti Rahayu Suryani ; Rurie Ratna Shantiningsih
Archives of Orofacial Sciences 2021;16(SUPP 1):43-49
ABSTRACT
Panoramic X-ray is well known to cause DNA damage and induces cellular death. The aim of the
present study was to evaluate the cytotoxicity of radiation exposure from panoramic radiography on
human buccal mucosa cells by assessing the cell viability using the simple-trypan blue exclusion test.
The genotoxicity effect was evaluated by assessing comet assay score. This research included a total of
20 healthy patients who had panoramic radiography for a routine dental examination. Buccal mucosa
cells were collected from all participants before X-ray exposure and at 30 min or 24 h after exposure in
Groups 1 and 2, respectively, and subjected to a comet assay and trypan blue exclusion test to assess
cell viability and DNA damage. Cell viability was calculated as the ratio of live (translucent) to total
counted cells. Comet assay output images were analysed using OpenComet software and a visual score
by measuring the percentages of tail DNA and summing the visual score, respectively. A statistically
significant (p < 0.05) reduce in cell viability was observed at 30 min after exposure, furthermore there is
no more reduction after 24 h. Both comet assay measurements showed a significant (p < 0.05) increase
in the percentage of tail DNA and visual score at 30 min after exposure, then tend to decrease after 24 h
of exposure, although it was not significant (p > 0.05). The results showed that panoramic radiography
interfered cell viability and induced DNA damage in buccal mucosa cells within 30 min after exposure,
but these effects were ceased after 24 h.
Mouth Mucosa--cytology
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Radiography, Panoramic