(MAP) on O2 uptake, CO2 production and rate of methemoglobin of human erythrocytes. The in vitro effects of methamidophos (MAP) on O2 uptake, CO2 production and rate of methemoglolin of human erythrocytes were studied: (1) after exposure of human erythrocytes to MAP at the concentrations from 1 to 10mM, only at concentration higher than 100mM of MAP in 37oC for 3 hours, the hemolysis was significantly induced. (2) at MAP concentrations between 1 and 10 mM, the significant increase of oxygen uptake and carbonic productions in human erythrocytes was seen. (3) At MAP concentrations between 1 and 10 mM, rate of methemoglobin in erythrocytes was increased significantly. These findings suggest that MAP act as oxidative chemical
methamidophos ; In Vitro

In Vitro Fertilization (IVF) is an established treatment approach for infertility based on the developments the past quarter century. However, there are still a number of reasons which prevent both doctors and patients from embarking on this available treatment option. The current indication for IVF (including endometriosis, tubal pathology, anovulation, male factor infertility-and unexplained infertility) are reviewed considering prognosis for pregnancy using IVF as compared to no/more conventional treatment. Evidence-based data are presented. Also, maternal age as a factor for success in IVF treatment is reviewed. Local setting situations which contribute to delays in treatment are also tackled.
FERTILIZATION IN VITRO

No abstract available.
Fertilization in Vitro* ; Fertilization* ; Humans*

No abstract available.
Fertilization in Vitro* ; Superovulation*

In vitro dose-response curve is presented for chromosome aberrations (dic.) in human lymphocytes exposed to acute cobalt-60 gamma radiation at different doses at dose rate of 0.3 Gy/min. The data showed that the relation between frequency of chromosome aberration and radiation dose, fitted to the linear quadric model: y = (4.88 +/- 0.57)x2 + (3.42 +/- 0.97)x + (0.06 +/- 0.08). This serves as basis of biological dosimetry
Lymphocytes ; In Vitro ; Chromosome Aberrations

In this study, we reported the possibility to enhance the "in vitro" and "in vivo" generation and cell (nuclear, nucleolar) activation of human and mouse T-lymphocytes by using Crinum latifolium (L.) (Vietnam) hot aqueous extracts (1-8mg/ml). For this purpose human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained and cultured in supplemented RPMI 1640 containing (1:3) a hot aqueous extract from Cirnum latifolium (L.) (Vietnam). Adherent and non adherent cells that represent stimulated/effectors T - cells were examined cytochemically and by the immunocyto chemical methods - for CD4+ and CD8+T - cell verification. The result obtained showed that this plant extract can efficiently stimulate human CD4+T cells in vitro. The same extract applied in vivo, using healthy Balb/c mice, has the capacity to induce lymphocytosis and a strong T - cell activation in the peripheral blood of experimental animals. Since some alkaloids like lycorine have been previously showed as active molecules in the hot aqueous extract of Crinum asisticum, we presume their and other biologically active substances capacity to induce in vitro and in vivo T-lymphocytes generation and T-cell blast transformation. The possible cellular - mechanisms of Crinum latifolium (L.) (Vietnam) hot aqueous extract simulative effects are discussed in relationships to T - cell immune mechanism in normal and pathological cases as neoplasias and leukaenmias.
Lymphocytes ; In Vitro ; Plants, Medicinal

The study on blood samples of 20 cancer patients in Hanoi Cancer Hospital and 10 healthy subjects showed that peripheral blood lymphocytes of cancer patients and healthy subjects contacting with low concentration H2O2 (10-8 mol) were damaged dramatically. At the present of HHKV extract 30 mcg/ml in same condition of oxygenated stress lymphocytes were protected partly. There was a higher statistically significant difference of imfulse number in these samples in comparing with no HHKV samples. Apart from antioxidant effect, HHKV had also a stimulant effect in the transformation of lymphocytes. The responses of lymphocyte samples with PHA in healthy persons were 8894.60701.49 and in cancer patients 1890.86459.30
Neoplasms ; Lymphocytes ; Stress ; In Vitro

No abstract available.
Fertilization in Vitro* ; Fertilization*

PURPOSE: The aim of this in vitro study was to evaluate the mechanical properties (bending strength and hardness) of seven different fiber reinforced composite posts, in relation to their microstructural characteristics. MATERIALS AND METHODS: Two hundred eighty posts were divided into seven groups of 40, one group for each type of post analyzed. Within each group, 15 posts were subjected to three-point bending strength test, 15 to a microhardess meter for the Knoop hardness, and 10 to Scanning Electron Microscope in order to determine the diameter of the fibers and the percentage of fibers embedded in the matrix. To compare the flexural strength in relation to the type of fiber, matrix, and the hardness of the posts, a Kruskal-Wallis H test was used. The Jonckheere-Terpstra test was used to determine if the volume percent of fibers in the post influenced the bending strength. RESULTS: The flexural strength and the hardness depended on the type of fibers that formed the post. The lower flexural strength of a post could be due to deficient bonding between the fiber and the resin matrix. CONCLUSION: According to the results, other factors, besides the microstructural characteristics, may also influence the mechanical properties of the post. The feature that has more influence on the mechanical properties of the posts is the type of fiber.
Hardness ; In Vitro Techniques

No abstract available.
Estradiol* ; Fertilization in Vitro*