(MAP) on O2 uptake, CO2 production and rate of methemoglobin of human erythrocytes. The in vitro effects of methamidophos (MAP) on O2 uptake, CO2 production and rate of methemoglolin of human erythrocytes were studied: (1) after exposure of human erythrocytes to MAP at the concentrations from 1 to 10mM, only at concentration higher than 100mM of MAP in 37oC for 3 hours, the hemolysis was significantly induced. (2) at MAP concentrations between 1 and 10 mM, the significant increase of oxygen uptake and carbonic productions in human erythrocytes was seen. (3) At MAP concentrations between 1 and 10 mM, rate of methemoglobin in erythrocytes was increased significantly. These findings suggest that MAP act as oxidative chemical
methamidophos ; In Vitro

In Vitro Fertilization (IVF) is an established treatment approach for infertility based on the developments the past quarter century. However, there are still a number of reasons which prevent both doctors and patients from embarking on this available treatment option. The current indication for IVF (including endometriosis, tubal pathology, anovulation, male factor infertility-and unexplained infertility) are reviewed considering prognosis for pregnancy using IVF as compared to no/more conventional treatment. Evidence-based data are presented. Also, maternal age as a factor for success in IVF treatment is reviewed. Local setting situations which contribute to delays in treatment are also tackled.
FERTILIZATION IN VITRO

No abstract available.
Fertilization in Vitro* ; Fertilization* ; Humans*

No abstract available.
Fertilization in Vitro* ; Fertilization*

The study on blood samples of 20 cancer patients in Hanoi Cancer Hospital and 10 healthy subjects showed that peripheral blood lymphocytes of cancer patients and healthy subjects contacting with low concentration H2O2 (10-8 mol) were damaged dramatically. At the present of HHKV extract 30 mcg/ml in same condition of oxygenated stress lymphocytes were protected partly. There was a higher statistically significant difference of imfulse number in these samples in comparing with no HHKV samples. Apart from antioxidant effect, HHKV had also a stimulant effect in the transformation of lymphocytes. The responses of lymphocyte samples with PHA in healthy persons were 8894.60701.49 and in cancer patients 1890.86459.30
Neoplasms ; Lymphocytes ; Stress ; In Vitro

In this study, we reported the possibility to enhance the "in vitro" and "in vivo" generation and cell (nuclear, nucleolar) activation of human and mouse T-lymphocytes by using Crinum latifolium (L.) (Vietnam) hot aqueous extracts (1-8mg/ml). For this purpose human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained and cultured in supplemented RPMI 1640 containing (1:3) a hot aqueous extract from Cirnum latifolium (L.) (Vietnam). Adherent and non adherent cells that represent stimulated/effectors T - cells were examined cytochemically and by the immunocyto chemical methods - for CD4+ and CD8+T - cell verification. The result obtained showed that this plant extract can efficiently stimulate human CD4+T cells in vitro. The same extract applied in vivo, using healthy Balb/c mice, has the capacity to induce lymphocytosis and a strong T - cell activation in the peripheral blood of experimental animals. Since some alkaloids like lycorine have been previously showed as active molecules in the hot aqueous extract of Crinum asisticum, we presume their and other biologically active substances capacity to induce in vitro and in vivo T-lymphocytes generation and T-cell blast transformation. The possible cellular - mechanisms of Crinum latifolium (L.) (Vietnam) hot aqueous extract simulative effects are discussed in relationships to T - cell immune mechanism in normal and pathological cases as neoplasias and leukaenmias.
Lymphocytes ; In Vitro ; Plants, Medicinal

In vitro dose-response curve is presented for chromosome aberrations (dic.) in human lymphocytes exposed to acute cobalt-60 gamma radiation at different doses at dose rate of 0.3 Gy/min. The data showed that the relation between frequency of chromosome aberration and radiation dose, fitted to the linear quadric model: y = (4.88 +/- 0.57)x2 + (3.42 +/- 0.97)x + (0.06 +/- 0.08). This serves as basis of biological dosimetry
Lymphocytes ; In Vitro ; Chromosome Aberrations

OBJECTIVES: This study aimed to compare the shear bond strength (SBS) of a self-adhering flowable composite (Dyad Flow) and a bulk-fill flowable composite (Smart Dentin Replacement [SDR]) to several pulp-capping materials, including MTA Plus, Dycal, Biodentine, and TheraCal.MATERIALS AND METHODS: Eighty acrylic blocks with 2-mm-deep central holes that were 4 mm in diameter were prepared and divided into 2 groups (n = 40 each) according to the composite used (Dyad Flow or SDR). They were further divided into 4 sub-groups (n = 10 each) according to the pulp-capping agent used. SBS was tested using a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed using 2-way analysis of variance. A p value of < 0.05 was considered to indicate statistical significance.RESULTS: A statistically significant difference (p = 0.040) was found between Dyad Flow and SDR in terms of bond strength to MTA Plus, Dycal, Biodentine, and TheraCal.CONCLUSIONS: Among the 8 sub-groups, the combination of TheraCal and SDR exhibited the highest SBS.
Dentin ; In Vitro Techniques ; Pemetrexed

OBJECTIVES: This paper presents a systematic review and meta-analysis of the effect of preheating on the hardness of nanofilled, nanoceramic, nanohybrid, and microhybrid resin composites. MATERIALS AND METHODS: An electronic search of papers on MEDLINE/PubMed, ScienceDirect, and EBSCOhost was performed. Only in vitro studies were included. Non-English studies, case reports, clinical trials, and review articles were excluded. A meta-analysis of the reviewed studies was conducted to quantify differences in the microhardness of the Z250 microhybrid resin composite using the Comprehensive Meta-Analysis software. RESULTS: Only 13 studies met the inclusion criteria for this systematic review. The meta-analysis showed that there were significant differences between the non-preheated and preheated modes for both the top and bottom surfaces of the specimens (p < 0.05). The microhardness of the Z250 resin composite on the top surface in the preheated mode (78.1 ± 2.9) was higher than in the non-preheated mode (67.4 ± 4.0; p < 0.001). Moreover, the microhardness of the Z250 resin composite on the bottom surface in the preheated mode (71.8 ± 3.8) was higher than in the non-preheated mode (57.5 ± 5.7, p < 0.001). CONCLUSIONS: Although the results reported in the reviewed studies showed great variability, sufficient scientific evidence was found to support the hypothesis that preheating can improve the hardness of resin composites.
Hardness ; In Vitro Techniques

No abstract available.
Estradiol* ; Fertilization in Vitro*