1.Characterisation of immunogenotypes of diffuse large B-cell lymphoma
The Malaysian Journal of Pathology 2019;41(2):101-124
Introduction: Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive type of non-Hodgkin lymphoma with variable clinical outcomes. The immunogenotypic features of this heterogeneous disease in Malaysia were not well characterized. Materials & Methods: In total 141 local series of DLBCL cases from UKM Medical Centre were retrospectively studied. Results: Of these cases, we classified our patients into two subtypes: 32.7% (37/113) GCB and non-GCB 67.3% (76/113) by Hans algorithm and the results showed strong agreement with the results by Choi algorithm (κ = 0.828, P<0.001). Survival analysis indicated significant difference in between GCB and non-GCB subtypes (P=0.01), elevated serum LDH (P=0.016), age more than 60-year-old (P=0.021) and the presence of B symptoms (P=0.04). We observed 12% DLBCL cases were CD5 positive and 81.8% of them died of the disease (P=0.076). Analysis on the dual expression of MYC/ BCL2 revealed that there is no significant difference in DE and non-DE groups (P=0.916). FISH study reported there were 9.22% (13/141) rearranged cases observed in our population at which highest frequency of BCL6 gene rearrangement (76.9%), followed by MYC (15.4%) and BCL2 (7.7%); no BCL10 and MALT-1 gene rearrangement found regardless of using TMAs or whole tissue samples. More cases of MYC protein overexpression observed compared to MYC translocation. Conclusion: Relatively lower frequency of GCB tumours and low gene rearrangement rates were observed in Malaysian population. A national study is therefore warranted to know better the immunogenotypic characteristics of DLBCL in Malaysia and their implications on the survival.
immunohistochemistry
2.Extrapancreatic primary retroperitoneal solid pseudopapillary neoplasm: A case report
Ashley T. Dionisio ; Bayani B. Tecson
Philippine Journal of Surgical Specialties 2023;78(2):53-60
Solid pseudopapillary neoplasm (SPN) is a rare pancreatic neoplasm.
This case reports a 63-year-old female previously diagnosed with
bilateral adrenocortical carcinoma post resection who presents
with persistent epigastric pain. Suspicions for recurrence prompted
diagnostics revealing retropancreatic and left suprarenal foci, both
suspicious for malignancy. Resection of both tumors yielded a
moderately to poorly differentiated retropancreatic carcinoma with
differentials not limited to recurrence and pancreatic neuroendocrine
tumor with a left suprarenal lymph node. Immunohistochemistry was
then done and was consistent with SPN having a strong expression for
b-catenin, vimentin, pancytokeratin. It was non-specific to melan-A
and negative for chromogranin A, synaptophysin, inhibin-a and CD10.
At 6 months’ follow up, patient is clinically well and abdominal CT
scan showed no recurrence. SPNs can masquerade as other neoplasms
having similar clinical, radiologic and histopathologic features.
Immunohistochemistry thus plays a crucial role for accurate diagnosis
and management. Surgical resection still remains the treatment of
choice and can provide a 95% overall survival rate, while limited
evidence supports the use of adjuvant chemotherapy or radiation.
Immunohistochemistry
3.Metastatic mammary carcinoma in a mature singleton placenta
Francia Victoria A . de los Reyes
Health Sciences Journal 2017;6(1):38-44
Introduction:
Placental metastasis from maternal malignancies is a rare occurrence with a
significantly adverse prognosis on the mother with no known effect or established risk factors for the
newborn. As such, characterization of these lesions is necessary to serve as a stepping stone for
more exhaustive studies regarding this presentation.
Case Summary :
This is a case of a metastatic breast carcinoma in a mature singleton placenta in
a 39 year old woman diagnosed with invasive breast carcinoma of no special type/invasive ductal
carcinoma, not otherwise specified, during the second trimester of pregnancy. Also discussed are the
immunohistochemistry studies done to confirm the origin of the tumor. A comparison of the ER, PR,
and HER2/neu receptor status between the primary lesion and the placental metastasis was also
done.
Conclusion
Pregnancy-associated breast cancer is a lesion that carries adverse prognosis for the
mother because of the delay in diagnosis attributable to confusion of symptomatology. The pertinent
problem in pregnancy-associated breast cancer with placental metastasis is the deficiency of the
placenta to induce tumor metastasis away from itself.
Immunohistochemistry
4.A study of CEA expression of the invasive colorectal adenocarcinoma on immunohistochemistry.
Gu KANG ; Chul Jae PARK ; Jae Jung LEE ; Hye Kyung AHN ; Young Hee CHOI ; Hyung Sik SHIN ; Min Chul LEE ; Young Euy PARK
Journal of the Korean Society of Coloproctology 1993;9(4):329-337
No abstract available.
Adenocarcinoma*
;
Immunohistochemistry*
5.A Case of Trichoblastoma.
You Jeong KIM ; Mi Yeon KIM ; Young Min PARK ; Hyung Ok KIM
Annals of Dermatology 2004;16(4):180-184
No abstract available.
Immunohistochemistry
;
Keratins
6.Light and electron microscopic immunocytochemistry of parafollicular cells in the Korean squirrel.
Wol Bong CHOI ; Chang Do CHOI ; Moo Hoo WON ; Nam Jung KIM
Korean Journal of Anatomy 1991;24(4):503-512
No abstract available.
Immunohistochemistry*
;
Sciuridae*
7.Decoded: A case report on dedifferentiated Liposarcoma on the Gluteal area
Juan Paolo David S. Villena ; Eileen Liesl A. Cubillan ; Ann Camille Q. Yuga
Acta Medica Philippina 2021;55(5):544-550
Dedifferentiated liposarcoma is a soft tissue sarcoma of adipocytic lineage. Histopathology and immunohistochemistry are essential for diagnosis. A 51-year-old Filipino woman presented with a rapidly enlarging left gluteal tumor. Histopathology revealed a multilobulated tumor having prominent myxoid stroma with numerous stellate-shaped, atypical cells bearing atypical mitotic figures. Other lobules were composed of sheets of pleomorphic cells, with atypical mitotic figures. The tumor stained positively with alcian blue, vimentin, MDM2 and p16 stains. Other immunohistochemical (IHC) studies done (pancytokeratin, CK7, CK 20, CD 34, CEA, desmin, EMA, SMA, S100) showed negative results. After a 2 cm wide excision of the sarcoma, patient was free from local tumor recurrence for 2 months, after which she was lost to follow-up. We report this case and a brief review of the current literature on dedifferentiated liposarcoma.
Liposarcoma
;
Immunohistochemistry
8.Pigmented extramammary Paget’s disease: A potential pitfall of misdiagnosis
Kristy Lydia S. Bunagan ; Janice Natasha C. Ng ; Maricarr Pamela M. Lacuesta ; Mary Jo Kristine S. Bunagan
Journal of the Philippine Dermatological Society 2019;28(2):50-54
Introduction:
Extramammary Paget’s disease (EMPD) is a rare cutaneous slow growing tumor seen in areas rich in
apocrine glands such as the anogenital region while ectopic EMPD is defined as EMPD arising on non-apocrine areas.
The pigmented variant of EMPD is a very rare finding, with only a few reported cases, and can be misdiagnosed as
melanoma.
Case report:
We report a case of a 74-year-old woman who presented with a four-year history of pruritic, non-
healing erythematous plaques located on the right axilla and left lower abdomen. Histopathology revealed
acanthotic epidermis with atypical keratinocytes that was negative for anti S-100 and Melan-A and was positive
for carcinoembryonic antigen (CEA), cytokeratin (CK), CK 7 and epithelial membrane antigen (EMA). Patient was
managed as pigmented and ectopic variant of extramammary Paget’s disease. Several tests and imaging were
done to rule out malignancy. Wide excision with axillary node dissection, bilateral inguinal node dissection, frozen
section biopsy and reconstruction using right pectoralis major musculocutaneous flap, split thickness skin graft with
left inguinohypogastric drain were done by reconstructive surgery. Frozen section biopsy was negative for S-100,
MELAN-A and HMB-45, ruling out malignant melanoma. Patient followed up every month for 6 months after the
procedure with no recurrence and lymphadenopathy.
Conclusion
This case emphasizes that extramammary Paget’s disease is not a preventable disease and early diagnosis
is the key to a favorable diagnosis. Any unilateral eczematous lesion that does not respond to an appropriate course
of topical treatment warrants a skin biopsy.
Melanoma
;
Immunohistochemistry
9.Quantitative Analysis of p53 Expression in Carcinoma of the Uterine Cervix.
Chi Seok AHN ; Eun Hwan JEONG ; Tae Soo LEE ; Rho Hyun SEONG
Korean Journal of Gynecologic Oncology and Colposcopy 1994;5(3):11-18
This studly was perormed 10 define the role of compulerized quantitative analysis in ewalualion for overexpression of p53 by immunohistochemistry. Total and ladeled cells wers counted automatically using commercially available software for color-image analysis. In 16 uterine cerwical carcinomas, the p53 ladeling index calculted bycomputerized5quantitatiwe analysis was 9.22%+/-8.70% and by visual analysis 5.90+/-6.51%. The present results suggest that the computerized quantitative analysis may be valuadle in objective interetation of immunohistochemical expression of p53 and reliable than conventional ways of visual analysis.
Cervix Uteri*
;
Female
;
Immunohistochemistry
10.Role of immunohistochemistry in female genital tract cancer.
Korean Journal of Gynecologic Oncology 2005;16(2):87-96
No abstract available.
Female*
;
Humans
;
Immunohistochemistry*