No abstract available.
Immunoenzyme Techniques*

No abstract available.
Fluoroimmunoassay* ; Immunoenzyme Techniques*

338 subjects at the age of 17-49 were undergone EIA test. Results showed an increasing trend in Chlamydia trachomatis infection. Clinical manifestations were not detected. The incidence is highest at the age of 17-26 (14.68%), 17-36 (10.67%). In the groups of genital tract inflamation, of uterine neck, of vaginitis the incidence reaches 2.7%, 12.71% and 12.50% respectively
Chlamydia trachomatis ; Immunoenzyme Techniques ; Women

No abstract available.
Immunoenzyme Techniques* ; Mycobacterium tuberculosis* ; Mycobacterium*

At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.
Chromatography ; Education ; Immunoenzyme Techniques ; Methods* ; Molecular Imaging

OBJECTIVES: To determine whether there is an association between the characteristics of pain in endometriosis and chronic stress. METHODS AND MATERIALS: Fifteen women were diagnosed as endometriosis by diagnostic laparoscopy and 14 asymptomatic volunteers were enrolled. Case group was divided into two groups with their pain duration and severity. Saliva was collected four times a day with commercial collector, Salivette. Salivary cortisol was analyzed by enzyme immunoassay. Statistical association was assessed with Kruskal-Wallis test, Mann-Whitney U test, and repeated measures ANOVA test. RESULTS: In the curve showing diurnal changes of cortisol level, morning rise in cortisol level was significantly blunted among patients compared with control group (p<0.05). Among two patient groups, there was no significant correlation with disease severity, but with duration (p<0.05). CA 125 level, pain scaling score, and age showed no significant correlation. CONCLUSION: Morning rise in salivary cortisol level is blunted in women especially with chronic endometriosis. These is findings might be a small clues that the changes in the salivary cortisol level can be a sign of chronic stress state.
Endometriosis* ; Female ; Humans ; Hydrocortisone ; Immunoenzyme Techniques ; Laparoscopy ; Saliva ; Volunteers

No abstract available.
Agglutination* ; Diagnosis* ; Female ; Immunoenzyme Techniques* ; Latex* ; Pregnancy ; Pregnancy, Ectopic*

BACKGROUND: All donated bloods collected by the Korean Red Cross Blood Centers are tested for anti-HCV (Hepatitis C Virus) antibody by enzyme immunoassay (EIA) kits made in Korea. EIA test has sustaining problem of false positivity in spite of great progress in manufacturing kits. So, many healthy donors have been reported as being infected with HCV and excluded from next donation. METHODS: Among blood samples of 2,040,151 donors which were tested by two kinds of EIA kits (DONG-A HCV 3.0 and LG HCD 3.0) from 16 blood centers during 12 months, repeatably reactive samples, total 6,851 samples, were supplementally tested by LG HCD CONFIRM immunoblot test. RESULTS: Positive, indeterminate and negative rate in immunoblot tests were 39%, 9%, and 12% respectively among 6,851 repeatably reactive samples. Estimated true positive rate of anti-HCV antibody in Korean blood donors was 0.13%, showing geographical difference between 0.03% and 0.46%. Of EIA repeatably reactive samples, 28% showed greater than 5 signal to cutoff (S/C) ratio and most of them (94%) was revealed to be positive. CONCLUSION: True positive rate of EIA test results is so low that it would be necessary to increase the confidence of such results by immunoblot tests.
Blood Donors* ; Humans ; Immunoenzyme Techniques ; Korea ; Red Cross ; Tissue Donors

BACKGROUND: Only 39% was the positive predictive value of anti-hepatitis C virus (HCV) antibody test done by Korean Red Cross. Supplemental enzyme immunoassay (EIA) by another EIA kit may be also effective for reporting the more correct result to donors, instead of expensive supplemental immunoblot test. METHODS: All repeatedly reactive blood samples by EIA from 16 regional blood centers were retested for anti-HCV antibody by Abbott IMx HCV kit and LG HCD CONFIRM immunoblot kit. Presence of viral RNA was also confirmed using Amplicor HCV TEST kit from 180 samples, which were proportionately selected according to supplemental EIA and Immunoblot results. RESULTS: Of 2,211 repeatedly reactive samples, 909 samples (41%) were reactive and 1,302 (59%) samples were non-reactive with IMx HCV kit. 81% of reactive samples also showed positive pattern on the LG HCD CONFIRM strips and 79% of 1,302 samples showed negative pattern. RNA positivity was estimated 66% and 17% in Abbott IMx HCV positive and negative samples respectively, and 72%, 6%, 20% in LG HCD CONFIRM positive, indeterminate and negative samples respectively. CONCLUSION: HCV RNA positivity in positive samples by Abbott IMx HCV or LG HCD CONFIRM was not statistically significant (z=0.57 < 1.96, alpha=0.05). RNA detection rate by Abbott IMx HCV or LG HCD CONFIRM among HCV RNA positive samples, which was estimated as 73%, 70% respectively, was also statistically insignificant (z=0.375 < 1.96, alpha=0.05). So, it seems to be a good and economical practice that donors are notified of anti-HCV antibody results after supplemental EIA test using Abbott IMx HCV kit.
Humans ; Immunoenzyme Techniques* ; Red Cross ; RNA ; RNA, Viral ; Tissue Donors*

BACKGROUND: Instead of hepatitis C virus (HCV) RNA test using RT-PCR, an enzyme immunoas-say for detection of HCV core protein as a simple, rapid method for the detection of HCV viremia has been developed recently. In this study, we investigated the usefulness of HCV core protein for the detection of HCV viremia by comparing the results of HCV RNA. METHODS: The study group included 71 patients; some of whom showed anti-HCV Ab. The HCV core protein assay was performed by enzyme immunoassay (Ortho Clinical Diagnostics, Raritan, NJ, USA). RESULTS: The concordance rate between HCV RNA and HCV core protein assay was 75%. Compared with the HCV RNA results, HCV core protein assay showed 50% sensitivity and 97% specificity. Among 17 patients whose results for HCV RNA were positive and those of HCV core protein were negative, all of them had anti-HCV Ab. CONCLUSIONS: Although the sensitivity of HCV core protein was not high in cases with anti-HCV Ab, the positive results for HCV core protein suggests the presence of HCV viremia. So, HCV core protein may be used as a simple and rapid method for detection of HCV viremia.
Hepacivirus ; Humans ; Immunoenzyme Techniques ; RNA ; Sensitivity and Specificity ; Staphylococcal Protein A ; Viremia*