Introduction: Tooth extraction is a regular method in dentistry in which it will result a lesion on the socket. The healing process of the lesion might lead to complication for instance bleeding, swelling, socket drying, and spreading of the infection. However, the healing process could be helped by the use of herbal medicine for it can reducing the complication risk. 90% freeze-drying Aloe vera gel, can grow the number of macrophages cells in which they play a significant role in the healing process. The purpose of this study was to determine an increasing number of macrophage cells in the wound healing process after tooth extraction after applying 90% freeze-drying Aloe vera gel. Method: freeze-drying Aloe vera consisted in CMC Na. Cavia cobaya divided into control group, day 1 and day 3, consists of the two groups without Aloe vera treatment and Treatment group, day 1 and day 3, consists of the two group treated with Aloe vera. Results: There is significant dissimilarity in both control and treatment group. In the treatment group, the number of macrophages between days 1 and 3 has been grown. It is because of the anti-inflammatory properties of Aloe vera. The activities and number of macrophages in tissue would be disrupted, if it is inhibited. Conclusion: 90% freeze-drying Aloe vera gel extract could increase number of macrophages in the healing process after tooth extraction on Cavia cobaya in observation days 1 and 3.