Introduction: Percutaneous vertebroplasty (PV) is one of the available treatments for vertebral compression fracture (VCF). Polymethylmethacrylate (PMMA) is the most common bone substitute used in the procedure, but it has several disadvantages. Bioceramic material, such as hydroxyapatite (HA), has better biological activity compared to PMMA. The aim of this study was to find an optimal biomaterial compound which offers the best mechanical and biological properties to be used in PV .M aterials and Methods: This was an experimental studyw ith goat (Capra aegagrus hircus)as an animal model. Thea nimals’ vertebral columns were injected with PMMA-HAc ompound. Animal samples were divided into four groups,a nd each group received a different proportion ofP MMA:HA compound. The mechanical and biologicale ffects of the compound on the bone were then analysed. Them echanical effect was assessed by measuring the vertebralb ody’s compressive strength. Meanwhile, the biologicale ffect was assessed by analysing the callus formation in thev ertebral body. R esults:The optimal callus formation and compressives trength was observed in the group receiving PMMA:HAw ith a 1:2 ratio.C onclusion: A mixture of PMMA and HA increases theq uality of callus formation and the material’s compressives trength. The optimum ratio of PMMA:HA in the compoundi s 1:2.