Aims: The present study aims at isolation and identifying termite gut bacteria from different Globitermes sulphureus mounds using a molecular approach based on16S rRNA genes. Methodology and results: The bacteria from the whole gut of soldier and worker castes of the termite G. sulphureus was isolated and identified. The diversity of the bacteria was compared between five different colonies in Universiti Sains Malaysia, Penang, Malaysia. The isolated bacteria were identified by using 16S rRNA gene sequencing method and subsequently used for phylogenetic analysis. Sequences analyses of identified bacteria were found to be affiliated with the phyla Firmicutes, Proteobacteria, and Actinobacteria. Soldiers and workers seemed to have little differences in bacterial species from the same colonies. We noted some bacteria which were detected in soldiers were not detected in workers. Conclusion, significance and impact of study: Differences in the culturable bacteria composition were not significant between termite colonies. However, the phylogenetic analysis revealed that most of the bacteria from one colony were slightly but distinctly different with bacteria from other colonies.

Heel pain is a common presentation at any outpatient clinic. Heel pain may originate from either bone or the surrounding soft tissues. Tendo achilles tendinitis and plantar fasciitis are the two most common causes of heel pain. We report a case of an uncommon condition presenting through a common presentation at the primary care clinic, i.e., an intraosseous lipoma of the calcaneum presenting through heel pain. This tumour constitutes 0.1% of benign bone tumours and is 8% prevalent in the calcaneum bone.

Aims: Rice blast disease caused by Pyricularia oryzae is one of the major biotic diseases of rice in Sarawak, Malaysian Borneo. This study aims to isolate and characterize rice blast fungus obtained from infected leaf collected from four different divisions in Sarawak, viz, Miri, Serian, Sri Aman, and Kuching. Methodology and results: Twelve succeeded isolates were pre-identified as P. oryzae by morphological characteristics of spores, followed by verification through (internal transcribed spacer) ITS sequencing. The isolates were evaluated for morphological characteristics, growth rate and sporulation rate, which were grown on two types of media, (filtered oatmeal agar) FOMA and (potato dextrose agar) PDA. Morphological characterization showed that the colony surface of the different isolates varied from smooth and fluffy to rough and flattened mycelia; some were with the present of concentric rings, and some with aerial mycelia. The growth rate and sporulation rate of each isolate varied based on types of media used. Most of the isolates grew faster on PDA than on FOMA but produced higher number of spores on FOMA as compared to PDA. Conclusion, significance and impact of study: This preliminary study showed that there were variations observed based on morphological and physiological characterization for the different isolates collected in Sarawak, Malaysian Borneo. This study is the first step towards understanding variation in the population of P. oryzae from Sarawak.

Waste and by-products form palm oil trees are versatile and can be used as biomass fuel, but the processes of producing electricity by using low pressure boilers are causing air pollution. The objective of this study is to determine the association between PM10 and PM2.5 exposure with respiratory symptoms and lung function among children living near to palm oil mill. A cross- Sectional comparative study was carried out among school children at Dengkil and Kerling. Questionnaires adopted from ATS-DLD-78-C were distributed to the respondents’ parents. PM10 and PM2.5 was measured. Lung function of the respondents was evaluated by using Chest Graph Spirometer; results were compared with standards of lung function, by ATS (1991). There was a significant difference for Mean±SD PM10 and PM2.5 in studied and comparative area, (p<0.05). There were significant association between PM10 with wheezing and cough (PR=5.220, CI%=1.030-26.453) and (PR=3.289 CI%=1.074-10.072). The study indicates that the lung function of; FEV1 % (t=-4.54, p=0.001) and FEV1/FVC % (t=-7.00, p=0.001) were lower among studied group compared to the comparative group. Results also showed that there is a significant inverse correlation between PM2.5 and FVC% (r=-0.352, p=0.0226). The need for palm oil mill management to evaluate the effectiveness of their implemented control measure seems to be very important, as black soot emitted from boilers stack is believed to contain high level of. The high efficiency biomass boiler and the used of fabric filters should be considered if the implemented control measure is not functioning effectively.

Rheumatic Heart Disease (RHD) has been thought as a disease of poor socioeconomic status. It is more prevalent in underdeveloped and developing countries than in developed countries. It is also common among the population with multiple social issues such as overcrowded dwellings, under-nutrition, poor sanitation and suboptimal medical care. This study was done to review the socio demographic profiles of RHD patients in Hospital Queen Elizabeth (HQE) ll, Kota Kinabalu, Sabah. A secondary data review of all patients registered under the RHD registry in HQE ll for one- year starting from July 2013 to June 2014. 204 RHD patients were included. Nearly three quarter (74.0%) were female. The mean age was 40.43 (14.75) years old. 61.1% has completed secondary education. 42.7% were housewives. The mean monthly income was RM 1363.83 (1297.05) which was categorized under the vulnerable income group. When they were categorized under the poverty level and the vulnerable income group, 42.6% and 76.5% of them fell under those categories respectively. The nearest health facilities to their houses were district hospitals (33.3%) with the mean distance of 9.17 km and health clinics (30.8%) with the mean distance of 4.27 km. Only 11.5% of them lived near the specialist hospitals with the mean distance of 21.32 km. Results from this review suggested that majority of RHD patients were in the low socioeconomic group with less access to health care facilities with specialist care. They are the most vulnerable groups and need to be prioritized in the specialized care program.​

Introduction: The Hedgehog (Hh) signalling pathway is a developmental signalling pathway involved in normal mammalian developmental and homeostasis of adult renewable tissues. In most adult tissues, this pathway remains silent and previous studies have shown that constitutive activation of Hedgehog signalling pathway leads to various types of malignancies including medulloblastomas, basal cell carcinoma, gastrointestinal, breast and prostate cancer. The purpose of this study was to investigate the immunohistochemical expression of Hedgehog pathway proteins in Diffuse Large B-cell Lymphoma and determine their association with overall survival (OS). Methods: Positive control using normal tonsils were included in each batch of immunohistochemical staining procedure. Results: PTCH1 proteins were highly expressed in DLBCL and showed strong staining intensity in 107 (100%) cases and SMO proteins were expressed in 105 (98.1%) cases. PTCH1 proteins were localised in the nucleus of tumour cells, whereas SMO proteins were mainly localised in the cytoplasm of tumour cells. Positive expression of PTCH1 and SMO proteins and overall survival of DLBCL patients were correlated with age, gender, race and tumour location. There was no significant correlation between the expression of these two proteins with any of the parameters. PTCH1 expression showed significant association with SMO expression (P=0.03). Conclusions: Our findings suggest that high expression of both PTCH1 and SMO may be important in the pathogenesis of DLBCL. However, additional mechanisms that may contribute to the activation of HH signalling in DLBCL needs to be further explored.

Safety is vital in any industry, including the offshore sector, which is classified as a major hazard industry. Health, Safet y and the Environment (HSE) identified that the prob a bility of accidents is high while working on the offshore sectors where it will exposed workers to many hazardous work activities. The appropriate measures to prevent acc i dent in this sectors must be laid out clearly. This paper is to identify the effectiveness of safety awareness campaign and the conti- nuity of the awareness among the workers to prevent injuries at of fshore. To achieve this, we have identified the level of awareness and propose a guideline on areas of improvement. Prior of embarking to offshore, staff w e re exposed to safety awareness pr o gram for four weeks. A f ter the program, we started with the pretest to all staff. They were posted off- shore for 6 weeks. Within the period, the performance awareness of each staff is monitored through observation and interview. During the f inal week, the posttest questionnaire were administered to all staff. Two instruments were used for the quantitative data collection, which are Unsafe Act Unsafe Condition (UAUC) card; and Behavior Observation Tool (BOT) card. Questio n naire data were analy zed quantitatively. Paired - sample t - test was used for analyzing pre and post result. The results show that the mean was increased. Recent studies on the safety briefing highlighted several significant changes in terms of e m ployee understanding toward safe ty. Safety awareness training has been introduced in the new safety briefing prior to of f shore mobilization.

Complementary Alternative Medicine (CAM) is broadly used as an adjunct treatment for patients to treat various kinds of disease, prevent disease and to sustain and enhance quality of life of the users. The study is aimed to investigate the association between CAM uses, Health-Related Quality of Life (HRQoL) and anthropometric indices among workers or staffs in Universiti Sultan Zainal Abidin (UniSZA) Gong Badak Campus, Kuala Terengganu. A total of 300 subjects participated in this study and completed the malay version of modified HRQol (SF-12) questionnaires. Anthropometric measurements among the subjects were done. Independent t-test, One-way ANOVA and Pearson correlation were used to test the association and the hypotheses. The prevalence of CAM users was 37.3%. The results showed that bodily pain score domain was significantly higher among non-CAM users. Overall, male had higher Mental Health Composite Score (MCS) score than female. Physical Health Composite Score (PCS) was only correlated negatively with BMI among non-CAM users but negative correlated with body fat percentage in both CAM and non-CAM users. As conclusion, HRQoL had significant negative correlation with body fat percentage among CAM users. No association was found between CAM use and HRQoL scores.