Aims: This study is focused on the potential of Bacillus cereus ATCC 14579 to degrade natural rubber (NR) latex film and NR latex film added with Metroxylan sagu pith waste (NR/TSPW latex film). Methodology and results: Bacillus cereus is proved capable to utilise rubber as main source of carbon and energy. The biodegradation studies were analyzed by growth profile, weight loss, protein content and tensile strength test. Fourier Transform Infrared Spectroscopy (FTIR) test was applied to confirm the biodegradation process. In shake cultures, OD of culture increased by 19.2% from the initial inoculum after 14th cultivation days with NR/TSPW latex film. An increase in protein content up to 0.037 mg/g with 12.377% weight loss of film was obtained after biodegradation. Tensile test result shows tensile strength and elongation break are decreased by 10.203%. Conclusion, significance and impact of study The demand of rubber products is increasing time to time. Due to the high consumable and disposable of rubber latex products, hence remain inert to degradation and leading to their accumulation in the environment. An attempt to combine Natural Rubber (NR) latex system with other degrading materials have been made to facilitate biodegradation process. Thus, Bacillus cereus ATCC 14579 has potency to provide a biotechnological solution to the waste rubber disposal problem.

Aims: This study is focused to explore Pseudomonas aeruginosastrain mass transport andassessment of the water quality.Methodology and results:Pseudomonas aeruginosahave been detected in the real location at the RBF study areabyusing molecular method. The bacterium strain was cultivated in the river water sample and column study was performed using soil aquifer collected from the RBF study site.Conclusions, significance and impact of the study:The column experiments show the removal of the bacteria was achieved almost 99%. Concentration of cultured bacteria was detected in range 0.26 g/Lto 0.33 g/Land reduced after flow through the soilpassage in range 0 to 0.148 g/L. This study also examines the water qualities that affected during the transport such as turbidity and color. The range of turbidity for initial concentration for both influents was 84-96 NTU was reduced to range 2.88-49.29 NTU. Meanwhile for color, the initial concentration was 51-58 PtCo was reduced to range 1-18 PtCo

Aims:Increasing of organic solvent waste contributed as one of the most critical environmental problems. Huge amount of solvents hasbeen applied in the industrial process, but it is not followed by a good waste treatment. Up to our knowledge only a few studieshas been conducted in applying the biological treatment on the solvent waste mixtures specifically by Gram’s positive organic solvent tolerant bacteria (OSTB). The study aims to identify the ability of OSTB survival in solvent waste mixture of the semiconductor industry in comparison to synthetic organic solvent by OSTB inoculation.Methodology and results:Strain of OSTB named as Bacillus subtilis, BSIAs was applied in the study. The growth of this OSTB in different concentration of synthetic solvent isopropyl alcohol (IPA) and in actual solvent waste mixture consists of IPA was monitored and measured. There are three different concentrations (v/v) of synthetic solvent IPA was used as a media that are 20%, 10% and 5% for testing the growth of B.subtilisBSIAs. The 5% concentration of IPA was suitable for B.subtilisBSIAs growth. After 14 hof growth, distillation process was used to separate the remaining solvent from the mixture. It was found that, the volume after biological treatment was reduced by 1 mL from the initial volume of solvent before the biological treatment. This OSTB also utilized solvent in 1% concentration of real solvent waste mixture within 120 h.Conclusion, significance and impact of study:As a conclusion, the findings reveal that the strain of Gram-positive B.subtilis,BSIAs has the ability to utilize synthetic organic solvent (IPA)and the solvent waste mixture from the semiconductor industry as their carbon sources. The selected OSTB can be considered as bio-agents in the industrial waste management pertaining to solvent waste problems thru green technology approaches.