Results of retrospective survey of some water-born diseases in people at 3 urban districts, Hai Phong city showed that: the prevalence of low infected diseases were typhoid, hepatitis, malaria, haemorrhagic fever, eye disease; diarrhea, intestinal amebiasis, helminth were high infection but tended to decrease from 1997 to 2002. The prevalence of haemorrhagic fever and malaria on 100,000 people in Hai Phong city decreased by the year, reduction 7-49 folds compared to the whole country and there were only sporadic cases. The prevalence of the highest diseases was in the age of 60 (7.5%)
Water ; Disease ; Urban Health ; Urban Population

In 2004, a project on environmental hygiene has been conducted at An Duong district to improve water supply system and sanitary latrines in the community. This study was designed for evaluating the project activities at two communes of An Duong, Hai Phong and some encouraging results have been yielded.
Environment ; Water ; Rural Health

Background:Asthma is a chronic infectious disease of respiratory which affect to health, study, labor and social activities. It can be occur at all age, the rate of incidence of this disease and mortality is increasing. Objectives:This study aims to learn about the clinical features and treatment results in children with asthma admitted into pediatric department, Thai nguyen Central Hospital. Subjects and method: A retrospective study was carried out on 65 asthma children who admitted to Asthma children of Department of Pediatrics, Thai Nguyen Central hospital in order to find the frequency, risks factor, clinical and management ofasthma from 1st January 2004 to 31st December 2006. Results:The asthma rate was 0.81 % among total inpatients and 2.87% among total patients admitted into the Respiratory Department. 30.8% of patients aged from 3 to 6 years old without sex predominance. The children were admitted more often in April, May, September, October and November. Personal and family history of asthma was seen in 70.77% and 35.38%, respectively. The common signs were: cough in 93.9%, coryza in 89.2%, intercostal retraction in 87.7%, wheezing in 80.0%, dyspnea in 66.2%. 4. Management: Bronchodilatator in 100%, antibiotics in 95.4%, corticoid in 76.9%. Improvement was seen in 98.5% and death in 54%. Conclusion: Drugs to lessen cough, phlegm and infusion were not encourage in asthma treatment.
Asthma/ pathology ; therapy ; Child

Background: Currently, the World Health Organization is encouraging developing countries to establish a seed lot system of rotavirus vaccine for production of this vaccine. Objectives: To determine gene sequences of rotavirus strain that was used for vaccine production and to evaluate its stability. Materials and method: Master seed (G4P6MS), Working seed (G4P6WS) and vaccine strain (G4P6VX) of Rotavirus were used for analysis at the US Center for Disease Control and Prevention (CDC). Results: 855 base pairs of gene 4 (VP4); 1195 base pairs of gene 6 (VP6); 824 base pair of gene 9 (VP7) and 715 base pairs of gene 10 (NSP4) from seed lot system and vaccines of G4P6 strain were determined. The results demonstrated this seed lot system is completely stable during vaccine production. There is no difference for nucleotide and amino acid sequence in this seed lot system. Conclusion: G4P6 strain (2001019203) is completely stable during vaccine production.
gene sequences ; seed lot system ; G4P6 (2001019203) ; rotavirus vaccine

Background: Presently, toxicity decreased oral live rotavirus is a candidate for vaccine for the prevention of rotavirus induced diarrhea. According to the World Health Organization, the seed lot system is robustly checked, in which determining the stable of gene sequence. Objective: To determine the sequence of genes 4: 6: 9: 10 with base pair correlative 855: 824: 1314: 734 of seed lot system G1P4 (2001019210) during production of rotavirus vaccine. Subject and methods: Gene 4 (VP4), gene 6 (VP6), gene 9 (VP7) and gene 10 (NSP4) of seed lot system G1P4 were determined for gene sequencing and then comparing the nucleotide sequence as well as deduced amino acids from original strain with the produced strain and vaccine virus. Results and Conclusion: There was no different for nucleotide and deduced amino acid sequence from the original strain during the production of rotavirus vaccine of G1P4 MS (2001019210) to producing strains of G1P4 WS and vaccine strains of G1P4 VX.
rotavirus vaccine ; gene sequence

Background: Rotavirus strain (KH0118) is used as the primary material to produce original rotavirus vaccine strains with the symbol of G1P8 MS. According to the World Health Organization\u2019s standard, the strain is needed to evaluate the stability of gene throughout analysis of gene and amino acid sequence during vaccine production. Objective: To determine the sequence of genes 4 (VP4), 6 (VP6), 9 (VP7) and 10 (NSP4) with base pair correlative 855:866:1345:745 of seed lot system and vaccine of G1P8 strain and to evaluate the stability of seed lot system during vaccine production. Subject and methods: ARN was divided from the original strain of rotavirus vaccine G1P8 MS, rotavirus vaccine productive strain (G1P8 WS) and rotavirus vaccine (G1P8 VX). Then using primer pairs to determine gene sequence VP4, VP6, VP7, NSP4 and comparing gene and amino acid sequence of the seed lot system. Results and Conclusion: The study demonstrated that, there was no difference for the nucleotide and amino acid sequence from the original strain during production of rotavirus vaccine G1P8 KH0118.
rotavirus vaccine ; G1P8 ; gene sequence

Background: Morphological structure of dental enamel-cement border of the 7th tooth is of great interest to many dentists with various different meanings. Study of the border of the 7th tooth allows the visual description of the morphological structure of this region. Objectives: To highlight the structure of dental enamel-cement border as well as its application in the endodontic treatment. Subjects and methods: The team used screening electronic microscopy to investigate the morphology and structure of twenty, second molar of adults who are between 18 and 35 year old. These teeth were extracted due to dental correction or caries (on chewing face). The study was conducted on both non-demineralized and demineralized teeth. Results: There are 3 kinds of connection in the enamel-cement border area of second molar: cement covering enamel, enamel-mineralized peridental ligament there is a gap between enamel and cement without exposing dentine. The kind of cement covering enamel connection is characteristic for dental interior face. The kind of enamel-mineralized peridental ligament is characteristic for the proximal, distal and middle dental face, the kind of enamel and cement without exposing dentine is characteristic for external face. Conclusion: Research conducted on only a small amount of tooth No. 7, further studies should be carried out on other teeth to have a more accurate description. \r\n', u'\r\n', u'
Morphological structure ; dental ; enamel -cement border of 7th tooth

Background: Rotavirus is the main cause of acute viral gastroenteritis in children under 5 years old. The virus leads to over 600000 children deaths a year in the world, 80% of which occur in the developing countries. In Viet Nam, 50%-70% the children\u2019s hospitalizations for acute diarrhea were resulted from rotavirus infection. Objective: To sequence nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8. Materials and method: A study was conducted in rotavirus sample of 5 passages of human rotavirus strain G1P8: B17A3; B17.3; B17.3 pp32vero15; B17.3 pp36TKP2; B17.3 pp43.7vero in Centre for Disease Control and Prevention, Atlanta, United State. Methods: using NucliSen Kit for detection of ARN; RT-PCR; sequencing genes by ABI 3100 machine. Results and Conclusion: Sequencing nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8 showed that: the number of nucleotide mutations ofVP4, VP7, NSP4 genes occurring among the passages were 3 (at nucleotit 175, 419, 790), 1 (at nucleotit 644), 3 (at nucleotit 134, 254, 482), respectively. All these mutations resulted in changes in amino acid composition. No mutation was found in NSP1 gene.
Rotavirus ; Genes ; Nucleotides/ genetics ;

Background: Rotavirus type A is the most common cause of acute gastrointestinal inflammatory in children under 5 years old, especially in age groups 6 and 36 months. Some rotavirus strains are common; seen recently in Vietnam are G1, G2, G3, G4 and G9, P4, P6 and P8. Objective: Surveillance of epidemiological characteristics of rotavirus induced diarrhea in the National Pediatric Hospital from September, 2007 to March, 2008. Subject and methods: Collection of 322 stool specimens of pediatric patients with acute diarrhea (including 213 specimens from male, 109 specimens from female), who were treated in the National Pediatric Hospital. All of these specimens were determined for causes of rotavirus with the enzyme immunoassay (EIA). Results and Conclusion: Among these 322 stool specimens, there were 195 rotavirus positive specimens, accounted for 60.56%. The rate of monthly distribution of rotavirus diarrhea from September, 2007 to March, 2008 were 76%, 56%, 62%, 61%, 64%, 56% and 44%, respectively. Number of rotavirus positive cases in male and female was 56 (26.29%) and 79 (72.48%), respectively. The rate of rotavirus positive children compared to total number of specimens with the age 0-3 months, 3-6 months, 6-12 months, 12-24 months, 24-36 months and over 36 months was 7.69%, 15.9%, 41.54%, 32.82%, 1.54% and 0.51%, respectively. The results of type identification indicated that phenotypes of 37 among 40 specimens were identified (92.5%) in which there were 5 specimens of G1P8 (12.5%), 20 specimens of G3P8 (50%), 1 specimen of G9P8 (2.5%), 2 specimens of G1Pmixed (5%), 9 specimens of G3Pmixed (22.5%), 1 specimen of G unidentified-type P8 (2.5%) and 2 specimens of G3 P unidentified-type (5%).
rotavirus ; diarrhea ; epidemiology

Background: Can Gio mangrove forest is bio-diversified area. There is few studies on medical arthropod in Can Gio mangrove forest until now. Objective: To study bio-diversified feature and identify species with disease transmitting role. Subject and Method: In 2007, medical arthropod surveys were conducted at 3 sites located in the natural conserve zone- Can Gio mangrove forest. A cross-sectional study was used. Results and Conclusions: A total of samples of 6178 individuals of medical arthropod belonging to 65 species have been collected, including 3 species of flea (Siphonaptera), 1 species of ticks (Ixodidae), 6 species of chiggers (Trombiculidae), 6 species of mites (Gamasoidea), 25 species of flies (Muscoidea) and 24 species of mosquitoes (Culicidae). They belong to 37 genus, 17 families, 3 orders (Siphonaptera, Acarina and Diptera), and 2 classes (Insecta and Arachnida). 20 species having epidemiological role were found in Can Gio mangrove forest. The rate of Anopheles epiroticus biting human at night indoor, outdoor and daytime indoor is rather high, especially at the end of dry season at centre of the forest.
Mangrove forest ; medical arthropod ; species ; genus